Sub-cellular localization and quantitative estimation of heavy metals in lemongrass plants grown in multi-metal contaminated tannery sludge

Pandey, Janhvi; Sarkar, Sougata; Verma, Rajesh; Singh, S. C.

doi:10.1016/j.sajb.2020.01.034

Cited by 7 publications

(2 citation statements)

References 14 publications

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“…EDX analyzes X-rays emitted from samples receiving a high-energy electron beam. This technique facilitates the qualitative and semi-quantitative detection of surface elements of samples and has been extensively used on various plant species such as sesame [23] and lemongrass [24].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Composition and Detection of Novel Bioactives in Anther Callus of Catharanthus roseus L.

Bansal

Mujib

Mamgain

et al. 2023

Plants

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Catharanthus roseus L. (G.) Don is the most widely studied plant because of its high pharmacological value. In vitro culture uses various plant parts such as leaves, nodes, internodes and roots for inducing callus and subsequent plant regeneration in C. roseus. However, till now, little work has been conducted on anther tissue using plant tissue culture techniques. Therefore, the aim of this work is to establish a protocol for in vitro induction of callus by utilizing anthers as explants in MS (Murashige and Skoog) medium fortified with different concentrations and combinations of PGRs. The best callusing medium contains high α-naphthalene acetic acid (NAA) and low kinetin (Kn) concentrations showing a callusing frequency of 86.6%. SEM–EDX analysis was carried out to compare the elemental distribution on the surfaces of anther and anther-derived calli, and the two were noted to be nearly identical in their elemental composition. Gas chromatography–mass spectrometry (GC–MS) analysis of methanol extracts of anther and anther-derived calli was conducted, which revealed the presence of a wide range of phytocompounds. Some of them are ajmalicine, vindolinine, coronaridine, squalene, pleiocarpamine, stigmasterol, etc. More importantly, about 17 compounds are exclusively present in anther-derived callus (not in anther) of Catharanthus. The ploidy status of anther-derived callus was examined via flow cytometry (FCM), and it was estimated to be 0.76 pg, showing the haploid nature of callus. The present work therefore represents an efficient way to produce high-value medicinal compounds from anther callus in a lesser period of time on a larger scale.

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Section: Discussionmentioning

confidence: 99%

Phytochemical Composition and Detection of Novel Bioactives in Anther Callus of Catharanthus roseus L.

Bansal

Mujib

Mamgain

et al. 2023

Plants

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“…The localization from epidermis, cortex, endodermis, pericycle to xylem elements were attributed to metal transport to above ground parts of the plant along with water and solutes. Additionally, the trichomes were swollen and larger in size at base, which was ascribed to a strategy for storage of more water, nutrients and essential oil (Pandey et al 2020).…”

Section: Heavy Metal Accumulation and Detoxification In Plant Trichomesmentioning

confidence: 99%

Functional aspects of solanaceae trichomes in heavy metal detoxification

Koul

Thomas

Karmakar³

2021

Nordic Journal of Botany

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Solanaceous plants are ubiquitous and are able to colonize degraded, abandoned and metal polluted habitats. Members of the family have evolved interesting extrinsic and intrinsic traits to combat the metal toxicity in multiple ways. Indumentum constituted mostly of trichome layer provides structural defense for both biotic stress (herbivory, oviposition, microbial pathogenesis) and abiotic stress (like drought, heat, freezing, photooxidation and UV radiation). Plants with heavy metal tolerance can accumulate toxic heavy metals in their trichomes as a part of their elemental defense detoxification strategy. Apart from the canonical functions, trichomes support ion and metal homeostasis, including the sequestration, compartmentalization, accumulation and excretion of heavy metals in many ways. The trichomes in Solanaceae are known to have a key role in the detoxification of Cd, Ni, Pb and Zn. Expressed sequence tag (EST)-based large-scale gene analysis in tobacco has indicated that trichomes are biologically active and stress-responsive structures. Trichome secretions and exudates also play an active role in metal detoxification process. Given that trichomes provide chemical defense from heavy metal stresses in the form of sequestration and extrusion, the plants having trichomes have potential role in remediation of contaminated soils. Solanaceae trichomes can serve as important model tissues that can be modified to attenuate metal toxicity, serve as environmental indicators and enhance phytoremediation potential of the family.

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