Stylar Basic Proteins Corresponding to 5 Self-Incompatibility Alleles of Japanese Pears.

Hiratsuka, Shin; Okada, Y.; Kawail, Yoshitaka; Tamura, Fumio; Tanabe, Kenji

doi:10.2503/jjshs.64.471

Cited by 22 publications

(13 citation statements)

References 14 publications

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“…Two S-protein bands, S2 and S4, out of 5 S-pro tein bands reported earlier (Hiratsuka et al 1995) were demonstrated to be transmitted to progenies, fulfilling one of the important criteria proposed by Gaude and Dumas (1987). Furthermore, incompatible pollen tubes ceased growing in detached styles, whereas compatible ones continued to elongate in the transmitting tissue.…”

Section: Discussionmentioning

confidence: 81%

“…Our data indicate that `OsaNjisseiki' contains a small amount of S4-protein and an equally scant level of an associated protein which is transmitted to its progenies. Furthermore, of the 16 progenies derived from `Osa-Njisseiki' x RNase activity of each S-protein seems to be proportional to the amount of respective proteins (Hiratsuka et al 1995). Thus, the relationship between the amount of S-protein in Japanese pears, including `Osa-Nijisseiki', and its role in recognition and/or rejection of pollen tubes has yet to be solved.…”

Section: Discussionmentioning

confidence: 99%

“…The proteins of styles from several progenies carrying the S2-and S4-allele from `Nijisseiki' and the S4sm-allele from `Osa-Nijisseiki' were extracted and analyzed by electrophoresis, using the protein and RNase band staining techniques described by Hiratsuka et al (1995).…”

Section: Protein Analysismentioning

confidence: 99%

“…

SummaryExpression and inheritance of style specific S2-and S4-allele associated S-proteins reported earlier (Hiratsuka et al, 1995) were studied using progenies of self-compatible 'Os a-Nijisseiki'.

…”

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confidence: 99%

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Expression and Inheritance of S-Proteins in Self-Compatible and -Incompatible Japanese Pears.

Hiratsuka

Okada

Kawai

et al. 1995

Engei Gakkai zasshi

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Protein Analysismentioning

confidence: 99%

“…

SummaryExpression and inheritance of style specific S2-and S4-allele associated S-proteins reported earlier (Hiratsuka et al, 1995) were studied using progenies of self-compatible 'Os a-Nijisseiki'.

…”

mentioning

confidence: 99%

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Expression and Inheritance of S-Proteins in Self-Compatible and -Incompatible Japanese Pears.

Hiratsuka

Okada

Kawai

et al. 1995

Engei Gakkai zasshi

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“…The Japanese pear, one of the most important fruit crops in Japan, has a strong gametophytic self-incompatibility mechanism, and sometimes shows cross-incompatibility among the cultivars. Seven alleles at the S-locus (Salleles) have been established using the paterclinal incompatibility phenomenon (Terami et al 1946) and, recently, proteins associated with the respective S-alleles (S-proteins) have been identified (Sassa et al 1992(Sassa et al , 1993Hiratsuka et al 1995;Ishimizu et al 1996). All of these proteins are RNases.…”

Section: Introductionmentioning

confidence: 98%

Selective inhibition of the growth of incompatible pollen tubes by S-protein in the Japanese pear

Hiratsuka¹,

Zhang

Nakagawa³

et al. 2001

Sexual Plant Reproduction

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The S-allele-associated proteins (S-proteins) in the styles of the Japanese pear (Pyrus serotina Rehd. var. culta Rehd.) were purified by cation exchange chromatography. Their inhibitory action on the growth of incompatible pollen tubes (pollen tubes bearing the same Sallele as in the style from which the S-proteins were prepared) was characterized in vitro. Germination and tube growth of self-pollen (pollen from the same cultivar from which the S-proteins were prepared) decreased dose-dependently when the S-protein was added to the medium. Tube length was reduced to 10% that of compatible pollen tubes (pollen tubes bearing the S-allele different from that in the style from which the S-proteins were prepared) at 1.5 µg µl 1 . S-proteins from Shinsui (S 4 S 5 ) also inhibited growth of cross-incompatible Kosui (S 4 S 5 ) pollen tubes, but not of compatible Chojuro (S 2 S 3 ) pollen tubes. After inactivation of RNase of the Sprotein, the inhibitory action of the S-protein disappeared. These results indicate that the S-protein acts directly to inhibit growth of incompatible pollen tubes in Japanese pear styles, and that the RNase activity of the protein is essential for the biological function. However, small amounts of proteins that co-migrated with the Sprotein may also play some roles in the inhibition. This is the first report on the selective inhibitory action of Sproteins in Rosaceae. Materials and methods Plant materialsAdult trees of the Japanese pear Pyrus serotina Rehd. var. culta Rehd. planted in the orchards of Mie University, Tsu, Japan, were used. The cultivars and their S-genotypes were: Chojuro (S 2 S 3 ), Osa-Nijisseiki (S s S 4 SM ), Nijisseiki (S 2 S 4 ), Shinsui (S 4 S 5 ) and Kosui (S 4 S 5 ). Osa-Nijisseiki is a self-compatible mutant and its S 4 SM allele is designated by progeny analysis of the cultivar (Sato et al. 1988). SM means stylar-part mutant, and S 4 SM -protein is the same as S 4 -protein . Flowers of each cultivar were collected immediately before anthesis, and styles were detached, weighed, and stored in liquid nitrogen until use. Anthers were also collected, dehisced, dried in a bottle containing desiccants, and stored at -30°C.

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Structural and molecular analysis of self-incompatibility in almond ( Prunus dulcis )

Certal

Almeida

Bošković

et al. 2002

Sexual Plant Reproduction

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A multi-approach was used to study different aspects of self-incompatibility (SI) in almond (Prunus dulcis). First, a population of almond cultivars was characterised as to their individual S-allele combination using separation of stylar protein extracts (non-equilibrium pH gradient electrofocusing) followed by staining for RNase activity, which led to the identification of one putative new allele and several new S-allele combinations. Second, a field pollination scheme was designed to study pollen tube progression and to obtain a spatial and temporal characterisation of this reproductive stage in both incompatible and compatible crosses. In addition, an antiserum was raised against a synthetic peptide designed from an almond S-protein (S 8 ) and used for immunological in situ detection in pistil cryosections. S-RNases were found to accumulate intercellularly in the stylar transmitting tissue as previously reported for other rosaceous species. The results are discussed in view of the evolution of the gametophytic SI system and the models proposed for its mechanism. Gametophyte selection is also proposed as an important intraspecific barrier to fertilisation in this species.

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Stylar Basic Proteins Corresponding to 5 Self-Incompatibility Alleles of Japanese Pears.

Cited by 22 publications

References 14 publications

Expression and Inheritance of S-Proteins in Self-Compatible and -Incompatible Japanese Pears.

Expression and Inheritance of S-Proteins in Self-Compatible and -Incompatible Japanese Pears.

Selective inhibition of the growth of incompatible pollen tubes by S-protein in the Japanese pear

Structural and molecular analysis of self-incompatibility in almond ( Prunus dulcis )

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