Expression and Inheritance of S-Proteins in Self-Compatible and -Incompatible Japanese Pears.

Hiratsuka, Shin; Okada, Y.; Kawai, Yoshitaka; Tamura, Fumio; Tanabe, Kenji

doi:10.2503/jjshs.64.479

Cited by 18 publications

(10 citation statements)

References 7 publications

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“…When the amount was calculated, Nijisseiki styles contained 3 times the concentration of S 4 -protein found in Osa-Nijisseiki at 2 DAA. This phenomenon supports the idea that Osa-Nijisseiki is a self-compatible mutant caused by modification of an S 4 -allele, which was indicated by genetic analysis on progenies of Osa-Nijisseiki (Sato et al 1988;Hiratsuka et al 1995b).…”

Section: Discussionsupporting

confidence: 82%

“…Although a more than 4-day lag occurs between Nijisseiki and Osa-Nijisseiki S 4 -protein expression, the manner of formation is quite similar in the cultivars. Further, the scanty level of S 4 -protein in Osa-Nijisseiki is confirmed to be transferred to progeny plants, accompanying the mutated S 4 -allele (Hiratsuka et al 1995b). Thus, this style-specific S 4 -protein from Osa-Nijisseiki seems to be a credible S-protein that fulfills most of the criteria of an S-protein as proposed by Gaude and Dumas (1987).…”

Section: Discussionmentioning

confidence: 84%

“…S-protein analyses also revealed that the quantity of S 4 -protein in Osa-Nijisseiki was low compared to that in Nijisseiki (Sassa et al 1992;Hiratsuka et al 1995b), and the low level of S 4 -protein was transferred to progeny of Osa-Nijisseiki carrying the mutated S 4 -gene (Hiratsuka et al 1995b). Nevertheless, Norioka et al (1996) recently reported that they could not obtain the cDNA coding for S 4 -protein from the stylar cDNA library of Osa-Nijisseiki and also failed to amplify the S 4gene by PCR using the cDNAs as a template.…”

Section: Extraction Of Poly(a) + and In Vitro Protein Synthesismentioning

confidence: 99%

“…After centrifuging the homogenate at 27 000 g for 15 min, RNAs in the supernatant were sedimented by LiCl at a final concentration of 2.5 M. The RNAs gathered by centrifugation at 20 000 g for 30 min were resolved with 10 mM Tris-HCl buffer (pH 7.5) containing 5 mM EDTA, 0.5 M NaCl and 0.5% SDS, and then passed through an oligo (dT)-cellulose column to purify the poly(A) + . 89 type such as in Nijisseiki (Furuta et al 1980), Kikusui (Hiratsuka et al 1987), Gion or Rokugatsu (Sato 1993); (3) the segregation ratio of progeny plants homozygous for the S 4 -allele to plants for heterozygous S 2 and S 4 is 1:1 in the progenies of selfed Osa-Nijisseiki (Sato et al 1988;Hiratsuka et al 1995b); and (4) plants homozygous for the S 2 -allele have never been found in selfed Osa-Nijisseiki progenies (Hiratsuka et al 1995b). …”

Section: Extraction Of Poly(a) + and In Vitro Protein Synthesismentioning

confidence: 99%

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Comparison of an S-protein expression between self-compatible and -incompatible Japanese pear cultivars

Hiratsuka¹,

Nakashima²,

Kamasaki³

et al. 1999

Sexual Plant Reproduction

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An S 4 -allele-associated protein (S 4 -protein) was identified by both isoelectricfocusing (IEF) polyacrylamide-gel electrophoresis and N-terminal amino-acid sequences in self-incompatible Nijisseiki (self-incompatibility genotype=S 2 S 4 ) and self-compatible Osa-Nijisseiki (S 2 S 4 SM , SM=stylar-part mutant) styles of the Japanese pear, Pyrus serotina Rehd. var. culta Rehd. Expression of the protein in the cultivars was compared during flower bud development and at post-transcriptional levels.In mature styles, S 4 -protein could be detected on IEF gel in both cultivars and the N-terminal amino acid sequences were identical, although Osa-Nijisseiki contained only one-third the amount contained in Nijisseiki. No difference was observed in S 2 -protien amounts. In Nijisseiki styles, S 4 -protein was already detectable 8 days before anthesis (DBA) and it was synthesized consistently until 2 days after anthesis (DAA); the amount increased 4.7-fold during these 10 days. In contrast, S 4protein in Osa-Nijisseiki was not detected earlier than 6 DBA; a small amount was found at 4 DBA, and it increased gradually as flowers developed. Thus, expression of Osa-Nijisseiki S 4 -protein is developmentally controlled in the same way as that of Nijisseiki S 4 -protein, but with a time lag of several days; the protein level at 2 DAA corresponded to that of Nijisseiki earlier than 4 DBA. S 4 -proteins from both Nijisseiki and Osa-Nijisseiki showed RNase activity and the activity was also developmentally controlled; it increased about fourfold during the interval from 8 DBA to 2 DAA in Nijisseiki, and 3.3-fold during the interval from 4 DBA to 2 DAA in Osa-Nijisseiki. Activity at 2 DAA, however, was twice as high in Nijisseiki. In vitro protein synthesis showed that poly(A) + from mature Osa-Nijisseiki styles could form S 4 -like protein in a manner similar to that of Nijisseiki. These results suggest that the self-compatibility of Osa-Nijisseiki is due to a low level of S 4 -protein expression, a mechanism very similar to the low level of S-protein and weak incompatibility in immature styles of self-incompatible Nijisseiki. Part of this protein repression may be regulated during post-transcriptional events.& k w d : Key words Japanese pear · Pyrus serotina · S-allele · Self-incompatibility · S-protein& b d y :

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 84%

Section: Extraction Of Poly(a) + and In Vitro Protein Synthesismentioning

confidence: 99%

Section: Extraction Of Poly(a) + and In Vitro Protein Synthesismentioning

confidence: 99%

See 2 more Smart Citations

Comparison of an S-protein expression between self-compatible and -incompatible Japanese pear cultivars

Hiratsuka¹,

Nakashima²,

Kamasaki³

et al. 1999

Sexual Plant Reproduction

Self Cite

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“…The S2-protein band detected in the basic zone of the gel seemed to be an S2-gene product because it was theoretically heritable by progeny plants carrying S2-allele (Hiratsuka et al, 1995). Therefore, the protein reported previously as a putative S 2-protein (Hiratsuka, 1992a), whose pI was 6.5 and N-terminal amino acid sequence was A(M)FTFTNXXPNTVGXG (Hiratsuka et al, 1992), may not be the S2-protein.…”

Section: Introductionmentioning

confidence: 98%

Stylar Basic Proteins Corresponding to 5 Self-Incompatibility Alleles of Japanese Pears.

Hiratsuka

Okada

Kawail

et al. 1995

Engei Gakkai zasshi

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Somaclonal Variation and Induced Mutations in Crop Improvement

Jain¹,

Brar²,

Ahloowalia³

1998

Current Plant Science and Biotechnology in Agriculture

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Aims and ScopeThe book series is intended for readers ranging from advanced students to senior research scientists and corpomte directors interested in acquiring in-depth, state-of-the-art knowledge about research findings and techniques related to all aspects of agricultural biotechnology. Although the previous volumes in the series dealt with plant science and biotechnology, the aim is now to also include volumes dealing with animals science, food science and rnicrobiology. While the subject matter will relate more particularly to agricultural applications, timely topics in basic science and biotechnology will also be explored. Some volumes will report progress in rapidly advancing disciplines through proceedings of symposia and workshops while others will detail fundamental information of an enduring nature that will be referenced repeatedly.The titles published in this series are listed at the end ofthis volume.

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Expression and Inheritance of S-Proteins in Self-Compatible and -Incompatible Japanese Pears.

Abstract: SummaryExpression and inheritance of style specific S2-and S4-allele associated S-proteins reported earlier (Hiratsuka et al., 1995) were studied using progenies of self-compatible 'Os a-Nijisseiki'.

Cited by 18 publications

References 7 publications

Comparison of an S-protein expression between self-compatible and -incompatible Japanese pear cultivars

Comparison of an S-protein expression between self-compatible and -incompatible Japanese pear cultivars

Stylar Basic Proteins Corresponding to 5 Self-Incompatibility Alleles of Japanese Pears.

Somaclonal Variation and Induced Mutations in Crop Improvement

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