Studying the Proteomic Composition of Expired Air Condensate in Newborns on Breathing Support

Kononikhin, Alexey; Ryndin, A. Yu.; Стародубцева, Н. Л.; Chagovets, Vitaliy; Burov, A.; Бугрова, А. Е.; Kostyukevich, Yury; Popov, Igor; Frankevich, Vladimir; Ionov, Ionov O.V.; Zubkov, Zubkov V.V.; Nikolaev, E. N.

doi:10.1007/s10517-016-3327-0

Cited by 3 publications

(4 citation statements)

References 8 publications

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“…The ability to analyse samples such as exhaled breath condensates requires a highly sensitive technique due to the low volumes available. Concentrations of a number eicosanoids were found to differentiate between the control group and the diseased lung groups highlighting an inflammatory aspect of the conditions [67]. Furthermore, eicosanoids were detected in 60% of all samples underlying the sensitivity of the nLC-nESI-MS approach used as previous analysis has shown these compounds are present at a pg/mL level in exhaled breath condensates [67,102].…”

Section: Health and Disease Metabolomicsmentioning

confidence: 83%

“…To date direct nLC has been implemented in a small number of metabolomic analysis of urine [62][63][64][65], plasma [11], tissue [23], faeces [66], exhaled breath condensates [67], sweat [68], cerebrospinal fluids [69] and cell extracts [70].…”

Section: Direct Flowmentioning

confidence: 99%

“…In human health and disease, four studies using nLC-nESI-MS have been carried out to date, one on HIV [64], one on drug resistant multiple myeloma [70], a third on paediatric spondylarthrosis [66] and the most recent investigating lung disease in new-born neonates [67]. The metabolomic analysis of anti-retroviral toxicity in the urine of HIV positive patients detected for the first time all the anti-retroviral drugs and a wide range of their metabolites in addition to several low abundance endogenous metabolites in an untargeted analysis (see Fig.…”

Section: Health and Disease Metabolomicsmentioning

confidence: 99%

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A review of nanoscale LC-ESI for metabolomics and its potential to enhance the metabolome coverage

Chetwynd

David

2018

Talanta

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Liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS) platforms are widely used to perform high throughput untargeted profiling of biological samples for metabolomics-based approaches. However, these LC-ESI platforms usually favour the detection of metabolites present at relatively high concentrations because of analytical limitations such as ion suppression, thus reducing overall sensitivity. To counter this issue of sensitivity, the latest in terms of analytical platforms can be adopted to enable a greater portion of the metabolome to be analysed in a single analytical run. Here, nanoflow liquid chromatography-nanoelectrospray ionisation (nLC-nESI), which has previously been utilised successfully in proteomics, is explored for use in metabolomic and exposomic research. As a discovery based field, the markedly increased sensitivity of these nLC-nESI platforms offer the potential to uncover the roles played by low abundant signalling metabolites (e.g. steroids, eicosanoids) in health and disease studies, and would also enable an improvement in the detection of xenobiotics present at trace levels in biological matrices to better characterise the chemical exposome. This review aims to give an insight into the advantages associated with nLC-nESI for metabolomics-based approaches. Initially we detail the source of improved sensitivity prior to reviewing the available approaches to achieving nanoflow rates and nanospray ionisation for metabolomics. The robustness of nLC-nESI platforms was then assessed using the literature available from a metabolomic viewpoint. We also discuss the challenging point of sample preparation which needs to be addressed to fully enjoy the benefits of these nLC-nESI platforms. Finally, we assess metabolomic analysis utilising nano scale platforms and look ahead to the future of metabolomics using these new highly sensitive platforms.

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Section: Health and Disease Metabolomicsmentioning

confidence: 83%

Section: Direct Flowmentioning

confidence: 99%

Section: Health and Disease Metabolomicsmentioning

confidence: 99%

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A review of nanoscale LC-ESI for metabolomics and its potential to enhance the metabolome coverage

Chetwynd

David

2018

Talanta

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“…This is of particular interest for preterm infants, as they cannot be regularly sampled for blood or urine analyses in sufficient volumes. 38,39,40,41 Publications of EBC analyzed from patients on respiratory support have continued to grow ever since. 42,20 This is equally important for assessment of EBC from critically ill mechanically ventilated patients with acute respiratory distress syndrome (ARDS).…”

Section: Exhaled Breath Condensate (Ebc)mentioning

confidence: 99%

Evolution of clinical and environmental health applications of exhaled breath research: Review of methods and instrumentation for gas-phase, condensate, and aerosols

Wallace

Pleil

2018

Analytica Chimica Acta

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Human breath, along with urine and blood, has long been one of the three major biological media for assessing human health and environmental exposure. In fact, the detection of odor on human breath, as described by Hippocrates in 400 BC, is considered the first analytical health assessment tool. Although less common in comparison to contemporary bio-fluids analyses, breath has become an attractive diagnostic medium as sampling is non-invasive, unlimited in timing and volume, and does not require clinical personnel. Exhaled breath, exhaled breath condensate (EBC), and exhaled breath aerosol (EBA) are different types of breath matrices used to assess human health and disease state. Over the past 20 years, breath research has made many advances in assessing health state, overcoming many of its initial challenges related to sampling and analysis. The wide variety of sampling techniques and collection devices that have been developed for these media are discussed herein. The different types of sensors and mass spectrometry instruments currently available for breath analysis are evaluated as well as emerging breath research topics, such as cytokines, security and airport surveillance, cellular respiration, and canine olfaction.

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Metabolomics of Exhaled Breath Condensate by Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry: A Methodological Approach

Maniscalco

Cutignano

Paris

et al. 2020

CMC

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: Respiratory diseases present a very high prevalence in the general population, with an increase in morbidity, mortality and health-care expenses worldwide. They are complex and heterogeneous pathologies that may present different pathological facets in different subjects, often with personal evolution. Therefore, there is a need to identify patients with similar characteristics, prognosis or treatment, defining the so-called phenotype, but also to mark specific differences within each phenotype, defining the endotypes. : Biomarkers are very useful to study respiratory phenotypes and endotypes. Metabolomics, one of the recently introduced “omics”, is becoming a leading technique for biomarker discovery. For the airways, metabolomics appears to be well suited as the respiratory tract offers a natural matrix, the Exhaled Breath Condensate (EBC), in which several biomarkers can be measured. In this review, we will discuss the main methodological issues related to the application of Nuclear Magnetic Resonance (NMR) spectroscopy and Mass Spectrometry (MS) to EBC metabolomics for investigating respiratory diseases.

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Studying the Proteomic Composition of Expired Air Condensate in Newborns on Breathing Support

Cited by 3 publications

References 8 publications

A review of nanoscale LC-ESI for metabolomics and its potential to enhance the metabolome coverage

A review of nanoscale LC-ESI for metabolomics and its potential to enhance the metabolome coverage

Evolution of clinical and environmental health applications of exhaled breath research: Review of methods and instrumentation for gas-phase, condensate, and aerosols

Metabolomics of Exhaled Breath Condensate by Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry: A Methodological Approach

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