Studying the candida resistance and sensitivity for some antifungals

Abstract

Back ground: The current study included 150 samples from patients with Candida from a hospital of Medical City / education labs - Baghdad, and ranged in age from (10 – 65(years for both sexes, all isolates taken from different sources (Lower respiratory tract, Urine, Skin, Vaginal and Oral thrux), where the number of males (55(as a percentage (37%) while the number of females (95) as a percentage (63%).
 The objective: The objectives of this study are studying resistance and sensitivity of candida spp to some antifungals.
 Material and methods: All the required material which used for the study, and the direct examination was conducted using potassium hydroxide KOH 10%, As well as microscopic examination and laboratory transplantation for all samples, for the purpose of investigating the candidiasis. Also Vitek System examination was conducted on all positive samples for microscopy and laboratory culturing; and so to be diagnosed on the species level, and this study included using (3) anti-fungal equipped from Himedia (India), an (Fluconazole, Clotrimazole and Nystatin), anti-fungal used by standard disk Diffusion to know the resistance of Candida and its sensitivity toward used anti-fungal.
 The results: The results of direct examination were vaginal swab (50), Sputum (28), Oral swab (34), Urine (21) and Skin swab (17).
 Conclusion: Candidiasis was more common in female patients as well as from medical units, where the rate of infection in females more than in males, Candida species were identified by the manual and automated methods and we found that the automated method by using VITEK2 YST Card was the best for species identification.

Evaluating the Antimicrobial Efficacy of Apple Cider Vinegar on Bacteria and Fungi Isolated from Vaginitis

“…Therefore, Antibiotics for vaginitis treatment must be carefully chosen so as not to eliminate the beneficial probiotics that play an essential role in keeping the vagina and its environment in a healthy order. The current study also agreed with the results of Hussein ' s study, Candida albicans that had the largest percentage of Candida species isolated from vaginitis, followed by Candida tropicalis and Candida parapsilosis [26].…”

Extraction of Klebsiella pneumoniae and Candida albicans Biofilm and Studying their Cytotoxic Effects on Human Lymphocytes

“…The band of the C=O carboxyl which was observed indicates the presence of sugar acids and a broad range of proteins (amide II) (Lal et al, 2010;Hussain et al, 2017). The absorption of the carbonyl amide bond is attributed to the presence of γ-lactone and ketone carbonyls (Wu et al, 2011;Hussein et al, 2019). These results confirm the presence of the lactone ring in biofilmproducing strains.…”

Enhancing the Antifungal Efficacy of Fluconazole with a Diterpene: Abietic Acid as a Promising Adjuvant to Combat Antifungal Resistance in Candida spp.

“…In fact, evidence indicates that some C. krusei cells may be sensitive to fluconazole, even in a dose-dependent manner [11]. This variability in resistance mechanisms also extends to the C. albicans and C. tropicalis species evaluated [49]. Individual susceptibility ultimately depends on the genetic composition of the microorganisms present in the colony used to prepare the inoculum for tests.…”