Study the effect of Antibiotics on pyocyanin production from Pseudomonas aeruginosa and pyocyanin as Antibiotic against different pathogenic bacteria

Sweedan, Enass Ghassan

doi:10.37652/juaps.2010.43881

Cited by 11 publications

(4 citation statements)

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“…Pyocyanin is known for its antibacterial, antifungal activity and biocontrol agent (Sweedan, 2010;Jayaseelan et al, 2014). As pyocyanin pigment is water-soluble, it has also been reported for its application in aquaculture as an effective agent against fungal diseases and the pathogen Vibrio sp.…”

Section: Introductionmentioning

confidence: 99%

Effect of culture conditions on pyocyanin production by recombinant pyocyanin-producing strain Pseudomonas aeruginosa PS39-phzMS

Nguyen¹,

Nguyen²,

Nguyen³

et al. 2023

MJM

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Aims: A suitable medium and cultivation parameters have an important role in the improvement of the production of pyocyanin pigment by Pseudomonas aeruginosa microorganism. The study aimed to optimize culture conditions and medium components for maximal pyocyanin production in a recombinant strain P. aeruginosa PS39-phzMS created in previous research. In addition, the process of extraction of pyocyanin was also investigated to select a proper applied solvent for recovering a high amount of pyocyanin as well as its quality. Methodology and results:The pyocyanin purification has based on solvent. Among six tested solvents for extracting pyocyanin out of bacterial broth, two out of six recovered a significant amount of pyocyanin, namely dichloromethane and chloroform, in which chloroform showed a higher pyocyanin yield (25.27 ± 1.02 µg/mL) than dichloromethane (20.26 ± 0.88 µg/mL). The thin-layer chromatography (TLC) of the extracted pyocyanin illustrated a similar to pure pyocyanin with Rf of 0.72 and no mark of other impurity metabolites. The UV-Vis spectra showed a similar peak at 520 nm with pure pyocyanin and the highest peak at 274 nm. Each single culture parameter was studied for the maximal production of pyocyanin. Next, a pyocyanin-producing GM medium was modified on the base of the KingA to find the relative capacity to biosynthesize high pyocyanin yield in P. aeruginosa PS39-phzMS. The results showed that pyocyanin production was the highest in optimal culture conditions, at 30 °C, pH 8, 120 h and agitation of 200 rpm. In the combination of culture condition with the GM, pyocyanin was created at the highest amount of 49.57 µg/mL. Conclusion, significance and impact of study: Based on the obtained results of the study, a pyocyanin-producing procedure was optimized, which suggests a promising application to scale-up pyocyanin production by the P. aeruginosa PS39-phzMS.

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Section: Introductionmentioning

confidence: 99%

Effect of culture conditions on pyocyanin production by recombinant pyocyanin-producing strain Pseudomonas aeruginosa PS39-phzMS

Nguyen¹,

Nguyen²,

Nguyen³

et al. 2023

MJM

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“…This result is in agreement with those obtained by Khadim and Marjani, who suggested that pyocyanin itself may play a direct role in the observed link between antimicrobial susceptibilities and the phenotype [ 38 ]. Furthermore, Sweedan suggested that pyocyanin production was relatively affected by antibiotic challenges [ 39 ]. In addition to the virulence factors produced by P. aeruginosa , it is also characterized by its ability to form biofilms.…”

Section: Discussionmentioning

confidence: 99%

Biofilm Formation, Pyocyanin Production, and Antibiotic Resistance Profile of Pseudomonas aeruginosa Isolates from Wounds

Chimi,

Noubom,

Bisso

et al. 2024

International Journal of Microbiology

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Pseudomonas aeruginosa is one of the most frequently resistant and dangerous bacteria isolated from infected wounds of patients. This study aimed to determine the prevalence of P. aeruginosa from infected wounds of patients in the Dschang District Hospital to evaluate their antibiotic susceptibility profiles and their ability to swarm and swim and correlate pyocyanin production with biofilm formation. Wound swab samples were collected and the identification of P. aeruginosa was performed using microbiological and biochemical tests. Their antimicrobial susceptibility was determined by the broth microdilution method. Swarming and swimming were determined by measuring the diameters of motility in semisolid/low-viscosity media. Furthermore, pyocyanin production and biofilm formation were evaluated spectrophotometrically using a microtiter plate. The prevalence of P. aeruginosa from infected wounds in our study population was 26%. All P. aeruginosa isolates were resistant to streptomycin and paromomycin, and the frequency of multidrug resistance (MDR) was 65.8%. All P. aeruginosa isolates showed the ability to produce biofilm and pyocyanin. Out of the 37 isolates screened, 19 including the reference strains (51.4%) were strong biofilm producers. A significant positive correlation was observed among biofilm formation, pyocyanin production, and the antibiotic resistance profile of the isolates. Findings from this study suggest that infected wounds could act as a reservoir for MDR and virulent P. aeruginosa. The presence of strong biofilm producers of P. aeruginosa in infected wounds is a serious public health concern. Therefore, surveillance programs to monitor and control MDR P. aeruginosa in these patients are required to prevent their dissemination in hospital settings.

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“…It showed bactericidal action against many pathogenic bacteria like Salmonella paratyphi, E. coli, and Klebsiella pneumonia 152 . The pigment showed very effectual activity against organisms like Acinetobacter, S. aureus, E. coli, and Streptococcus pneumonia 160 . Pyocyanin pigment also showed antibacterial action on food spoilage bacteria like L. monocytogenes and B. cereus.…”

Section: Pyocyanin As Antimicrobial Agentmentioning

confidence: 99%

Challenges and Opportunities with Pyocyanin Pigment of Pseudomonas aeruginosa

Rani,

Azmi

2020

J. Adv. Mircobiol.

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Microbial secondary metabolites are the organic compounds that are not directly involved in the normal growth, development, or reproduction of an organism but very useful for human health. These are formed during the stationary phase (idiophase) of culture, also known as idiolites such as pigments, toxins, alkaloids, antibiotics, etc, and have a very unusual structure. Production of secondary metabolites occurred when there is depletion of one or more nutrients in the culture medium. Microbial pigments such as carotenoids, melanin, prodigiosin, violacein, pyocyanin, etc, are natural colors that hold promising potential to meet various challenges in the pharmaceutical and nutraceutical sector. Pyocyanin is one of many natural pigments, a blue-colored redox-active secondary metabolite, which is produced by Pseudomonas aeruginosa. Several virulence factors are secreted by P. aeruginosa that can contribute to its pathogenicity and pyocyanin pigment is one of them. It causes oxidative stress to the host, disrupting host catalase, mitochondrial electron transport, induces apoptosis in neutrophils as well as inhibits the phagocytosis of apoptotic bodies by macrophages. It is challenging for anti-virulence drug intervention to control the pathogenesis of P. aeruginosa to some extent by targeting the biosynthetic pathway of pyocyanin. Besides this, pyocyanin pigment possesses antioxidant, antimicrobial, anti-inflammatory, anticancerous, and immunosuppressive properties. This article aims to describe challenges posed by pyocyanin pigment of P. aeruginosa and its potential applications in various fields of biotechnology.

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Study the effect of Antibiotics on pyocyanin production from Pseudomonas aeruginosa and pyocyanin as Antibiotic against different pathogenic bacteria

Cited by 11 publications

References 0 publications

Effect of culture conditions on pyocyanin production by recombinant pyocyanin-producing strain Pseudomonas aeruginosa PS39-phzMS

Effect of culture conditions on pyocyanin production by recombinant pyocyanin-producing strain Pseudomonas aeruginosa PS39-phzMS

Biofilm Formation, Pyocyanin Production, and Antibiotic Resistance Profile of Pseudomonas aeruginosa Isolates from Wounds

Challenges and Opportunities with Pyocyanin Pigment of Pseudomonas aeruginosa

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