Study on urinary metabolic profile of phenylketonuria by micellar electrokinetic capillary chromatography with dual electrochemical detection—Potential clinical application in fast diagnosis of phenylketonuria

Detection of Phenylketonuria Markers Using a ZIF-67 Encapsulated PtPd Alloy Nanoparticle (PtPd@ZIF-67)-Based Disposable Electrochemical Microsensor

“…44,45 The abnormal accumulation of these markers in PKU patients is usually about 10 times or even much higher than the normal levels. 9,45 Thus, the measurements of phenylpyruvic acid and phenylacetic acid over the physiological range have been conducted. From Figure 6a,b, the amperometric detection of phenylpyruvic acid displays a linear signal increase with a gradual increase in the concentration.…”

“…According to the literature studies, the urinary content of phenylpyruvic acid in healthy newborns is about several μM, and phenylacetic acid usually has a higher level than that of phenylpyruvic acid. , The abnormal accumulation of these markers in PKU patients is usually about 10 times or even much higher than the normal levels. , Thus, the measurements of phenylpyruvic acid and phenylacetic acid over the physiological range have been conducted. From Figure a,b, the amperometric detection of phenylpyruvic acid displays a linear signal increase with a gradual increase in the concentration.…”

Contactless conductivity detection for analytical techniques: Developments from 2010 to 2012

“…Other small ions, such as fluoride and fluoroacetate and a range of organic acids , were also analyzed by CE‐C 4 D. It was also shown that CE‐C 4 D can be applied in the determination of increasing concentrations of plasma lactate during exercise testing . Phenylated organic acids were monitored in urine samples as a potential diagnostic tool for determination of phenylketonuria using MEKC with dual C 4 D/amperometric detection . Carnitine and acylcarnitines were determined in urine and plasma samples , and creatine and phosphocreatine were assessed in muscle biopsy samples .…”

Recent advances in applications of capillary electrophoresis with capacitively coupled contactless conductivity detection (CE‐C4D): an update

“…Zhang et al . () proposed electrokinetic capillary chromatography with C 4 D and AD method (MECC‐C 4 D/AD) for the fast determination of urinary marker compounds of phenylketonuric. The separation was performed in a 35 mmol/L SDS/60 mmol/L H 3 BO 3 –Na 2 B 4 O 7 running buffer (pH 8.2) at voltage of 16 kV.…”