Study on Tissue Tropism and Molecular Detection of VP2 Gene of Infectious Bursal Disease Virus in Experimentally Infected Broiler Tissues

Detection and Phylogenetic Analysis of Infectious Bursal Disease Virus Based on both Genome Segments A and B Isolated from Backyard Poultry Punjab, Pakistan

“…Viral RNA was extracted from the bursa homogenate using QIAamp Viral RNA Kit (Qiagen, Germany) as directed by the manufacturer. Viral cDNA was synthesized using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, USA) (Zahid et al, 2016). The selected DNA fragment was amplified through PCR in a 25µL reaction mixture, containing 12.5µL master mix (PrimeSTAR Max DNA polymerase, Catalog Number: R045A), 1µL DNA template, 1µL each forward and reverse primers and 9.5µL PCR grade water.…”

“…Pathogenic IBDV is classified into four subtypes, i.e., attenuated, classical virulent, antigenic variants, and very virulent IBDV (Hussain et al, 2020). IBDV targets immature B lymphocytes (Zahid et al, 2018) in the lymphoid organ (bursa of Fabricius), leading to age-dependent immunosuppression exposing birds to other opportunistic pathogens. Very virulent IBDV (vvIBDV) has been reported in different countries with more than 70% chicken mortality, posing a severe threat to the poultry industry (Dey et al, 2019).…”