Detection and Phylogenetic Analysis of Infectious Bursal Disease Virus Based on both Genome Segments A and B Isolated from Backyard Poultry Punjab, Pakistan

Waheed, Syeda Fakhra; Aslam, Asim; Khan, Muhammad Aamir; Ashraf, Kamran; Zahid, Beenish

doi:10.17582/journal.pjz/20211230141208

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…Each FMD virus population such as field isolate is genetically and antigenically heterogenous, consisting of a spectrum of variants and termed them quasispecies [32]. The main immunogenic site of FMD viruses is located on the 1D region, which makes the VP1 region, the gene of choice to study variation and relationships between the different isolates [33,34]. However, a regular mass vaccination and the achievement and maintenance of an FMD free status have major benefits for International trade.…”

Section: S No Fmdv Serotype a Isolatesmentioning

confidence: 99%

Molecular Characterization and Phylogenetic Analysis of Field Isolates of Foot-and-Mouth Disease Virus Serotype A from Uttar Pradesh, India

Singh,

Kumar,

Verma

et al. 2023

Act Sci VetSci

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The rapid response to the economically important livestock diseases is greatly aided by the surveillance for the circulating etiological agent. Among these, foot-and-mouth disease in India is considered an important disease caused by Apthovirus of picornaviridae family. Continuous studies on the genetic and antigenic characterization of the circulating FMDV serotypes are absolutely essential due to the high mutation rate of the virus. The present study was conducted to determine the molecular characterization and phylogenetic analysis of a FMDV serotype A circulating in the western Uttar Pradesh through partial sequencing of VP1 gene. Vesicular epithelium samples were collected from different outbreaks of FMD and processed for extraction of viral RNA and RT-PCR. DNA sequence analysis was performed and phylogenetic tree was constructed to determine the phylogenetic relationship. Out of 25 tongue epithelium samples analyzed, the amplicons (1301 bp and 865 bp) could be amplified from 20 tongue epithelium (12 FMD serotype O and 8 FMD serotype A). Sequence and phylogenetic analyses of partial 1D gene of two FMDV serotype A samples showed the genetic variation from the previously reported FMDV in India. In conclusion, there is need of continuous monitoring of FMDV mutations and evaluation of the FMD vaccines strains currently used in India to avoid the recurrence of FMDV outbreaks.

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Section: S No Fmdv Serotype a Isolatesmentioning

confidence: 99%

Molecular Characterization and Phylogenetic Analysis of Field Isolates of Foot-and-Mouth Disease Virus Serotype A from Uttar Pradesh, India

Singh,

Kumar,

Verma

et al. 2023

Act Sci VetSci

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“…The disease affects lymphatic tissues like the bursa of Fabricius, spleen, etc., and results in lymphoid depletion in the bursa of Fabricius (Getachew and Fesseha 2020; Amajo et al 2022). The virus belongs to the Birnaviridae family (Mahmoud et al 2019;Waheed et al 2022). Cosgrove (1962) reported a specific disease (IBD) that affects the bursa of Fabricius in chickens.…”

Section: Introductionmentioning

confidence: 99%

Infectious Bursal Disease (Gumboro) in Backyard Chicken in Jordan

2023

Int J Vet Sci

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Infectious bursal disease (IBD) is an acute and highly infectious and contagious disease that affects young chickens. The current study aims to determine the seroprevalence of IBD in healthy, non-vaccinated male chickens raised as backyard chickens and histopathological changes. We collected 210 tissues and blood samples from backyard chickens in Amman, Jordan. Indirect ELISA was applied to test serum samples for IBD virus antibodies. Morbid tissue samples of livers and spleens were processed for histopathological examination. The study revealed 80.95% prevalence rates of infectious bursal disease virus (IBDV) antibodies. Seropositivity to IBDV did not vary significantly between the study sites. It was concluded that IBD is endemic and broadly appeared in the studied areas. Histopathological study revealed that IBD rendered depletion of lymphoid tissue in the spleen. The franked disease did not occur in adult and older chickens, though those were also seropositive for IBD virus antibodies.

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Detection and Phylogenetic Analysis of Infectious Bursal Disease Virus Based on both Genome Segments A and B Isolated from Backyard Poultry Punjab, Pakistan

Abstract: Authors' Contribution SFW conducted the research. AA supervised the study and AA, MRK and KA designed the study. SFW wrote the manuscript. BZ helped in interpretation of results and article writing.

Cited by 2 publications

References 27 publications

Molecular Characterization and Phylogenetic Analysis of Field Isolates of Foot-and-Mouth Disease Virus Serotype A from Uttar Pradesh, India

Molecular Characterization and Phylogenetic Analysis of Field Isolates of Foot-and-Mouth Disease Virus Serotype A from Uttar Pradesh, India

Infectious Bursal Disease (Gumboro) in Backyard Chicken in Jordan

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