Study on the value of antibiotic‑resistant gene detection in Helicobacter pylori in China

Abstract

The aim of the present study was to explore the value of detecting antibiotic-resistant genes in Helicobacter pylori (H. pylori) and the association between genotype and antibiotic resistance. Two gastric mucosa samples from each H. pylori-positive patient were collected. Each patient's H. pylori sample was cultured in vitro, and the agar plate dilution method was conducted. In addition, all patient samples were analyzed for the detection of antibiotic resistance-related mutant genes and VacA gene genotypes. The association between VacA genotypes and antibiotic resistance was also determined and the value of mutant gene detection in predicting H. pylori resistance to antibiotics was evaluated. In total, 133 H. pylori-positive patients were enrolled. A total of 22 strains of H. pylori failed to grow in in vitro culture and 25 strains were negative in a H. pylori gene test. Among 108 strains detected by PCR, a total of 39 VacA s1m1 strains, 69 VacA s1m2 strains and no VacA s2 strain were identified. There was no significant association between VacA genotypes and antibiotic resistance. The mutation rates of G616A in the rdxA gene, T87A, G91A, A91G and G91T in the gyrA gene and A2143G and A2142G in the 23S rRNA gene were 32. 1, 32.3, 22.6, 12.9, 6.5, 81.8 and 0.0%, respectively. Among these mutant sites, the mutation coincidence rates were as follows, according to the agar plate dilution method: rdxA G616A (81.8%), gyrA G91T (66.7%), gyrA G91A (54.5%), 23 S rRNA A2143G (49.1%), gyrA T87A (45.5%), gyrA A91G (33.3%), penicillin-binding protein 1 (PBP1) C556G (0.0%), PBP1 A562T (0.0%), PBP1 A562G (0.0%) and 16 S rRNA 926-927 (AT-GT) (0.0%). VacA m subtypes were not associated with H. pylori antibiotic resistance. In conclusion, the present findings suggested that the detection of related mutant genes had a clinical application value in predicting the antibiotic resistance of H. pylori, particularly resistance to clarithromycin and levofloxacin.

Helicobacter pylori Virulence Factors and Clarithromycin Resistance-Associated Mutations in Mexican Patients

“…This result is not in agreement with Agudo et al, who reported that clarithromycin resistance in H. pylori isolates was strongly associated with the vacA s2/m2 genotype; however, no association with 23S rRNA gene mutations and vacA/cagA genotypes is reported [ 21 ]. Otherwise, different studies have not found an association between vacA genotypes and antibiotic resistance [ 57 , 58 ]. Therefore, multicenter studies are needed to study the relationship between clarithromycin resistance-associated mutations and vacA/cagA genotypes of H. pylori.…”

Novel therapeutic regimens against Helicobacter pylori: an updated systematic review

“…H. pylori has strict oxygen requirements. The optimal oxygen concentration range in air is 5 to 8% ( Dai et al, 2022 ), which is equal to 1.7 to 2.0 mg/L in aqueous solutions at 37°C. When the oxygen concentration in the environment is outside this range, H. pylori cannot survive.…”

New recording genes and genetic mutations of H. pylori genome in gastroduodenal disease patients in Basrah province-Iraq

“…Vianna et al, (2018) identified seven mutation in 23s rRNA region related to antibiotic resistance, whereas the results of Jabar & Alfaisal, (2017) documented no mutations were observed in the codons but the sequencing analysis detect point mutations in exons (17,18). In a study by Dai et al, (2022) which enrolled a total of 133 H. pylori positive patients they reported that no significant link was detected between VacA genotypes patients and antibiotic resistance (19). Aziz et al, (2022) detect a number of specific virulence proteins in gastric biopsies of gastroduodenal disease H. pylori infected individuals, these proteins were controlled by CagA pathogenicity island (20).…”