Study on the molecular interaction of graphene quantum dots with human serum albumin: Combined spectroscopic and electrochemical approaches

Huang, Shan; Qiu, Hangna; Lu, Shuangyan; Zhu, Fawei; Xiao, Qi

doi:10.1016/j.jhazmat.2014.11.019

Cited by 108 publications

(52 citation statements)

References 49 publications

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“…It is deemed to be caused by electron transitions from a π orbital to the lowest unoccupied molecular orbital. The photometric property of GQDs is linked to assignment of an n − π* electronic transition in term of a previous study (Huang et al, ). The XPS of G,GO and GQDs showed two peaks at 284 and 532 eV, which were, respectively, corresponding to C1s and O1s spikes (Fig.…”

Section: Resultsmentioning

confidence: 96%

Chronic exposure to graphene-based nanomaterials induces behavioral deficits and neural damage inCaenorhabditis elegans

et al. 2017

J. Appl. Toxicol.

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Nanomaterials of graphene and its derivatives have been widely applied in recent years, but whose impacts on the environment and health are still not well understood. In the present study, the potential adverse effects of graphite (G), graphite oxide nanoplatelets (GO) and graphene quantum dots (GQDs) on the motor nervous system were investigated using nematode Caenorhabditis elegans as the assay system. After being characterized using TEM, SEM, XPS and PLE, three nanomaterials were chronically exposed to C. elegans for 6 days. In total, 50-100 mg l GO caused a significant reduction in the survival rate, but G and GDDs showed low lethality on nematodes. After chronic exposure of sub-lethal dosages, three nanomaterials were observed to distribute primarily in the pharynx and intestine; but GQDs were widespread in nematode body. Three graphene-based nanomaterials resulted in significant declines in locomotor frequency of body bending, head thrashing and pharynx pumping. In addition, mean speed, bending angle-frequency and wavelength of the crawling movement were significantly reduced after exposure. Using transgenic nematodes, we found high concentrations of graphene-based nanomaterials induced down-expression of dat-1::GFP and eat-4::GFP, but no significant changes in unc-47::GFP. This indicates that graphene-based nanomaterials can lead to damages in the dopaminergic and glutamatergic neurons. The present data suggest that chronic exposure of graphene-based nanomaterials may cause neurotoxicity risks of inducing behavioral deficits and neural damage. These findings provide useful information to understand the toxicity and safe application of graphene-based nanomaterials. Copyright © 2017 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 96%

Chronic exposure to graphene-based nanomaterials induces behavioral deficits and neural damage inCaenorhabditis elegans

et al. 2017

J. Appl. Toxicol.

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“…The reported fluorescence quenching mechanisms of a collisional process (dynamic quenching) and ground state complex formation (static quenching) between the quencher and fluorophore can be easily distinguished by difference in temperature dependence (Huang et al, 2015). Assuming that the fluorescence quenching occurred by dynamic quenching, which depends on the interaction between the quencher and fluorophore, the linear relationship between [C n mim]Cl and ctDNA can be calculated using the following Stern-Volmer equation (Fotouhi et al, 2013;Zhuang et al, 2016):…”

Section: Fluorescence Spectroscopic Studiesmentioning

confidence: 99%

Evaluation of interaction between imidazolium-based chloride ionic liquids and calf thymus DNA

Liu

Dong

et al. 2016

Science of The Total Environment

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“…The enhancement of R ct might be attributed to intercalative binding between the ctDNA and drug, which subsequently increased the electric resistance of the ctDNA‐modified gold electrode. Furthermore, the affinity constant between the drug and ctDNA in EIS can be calculated according to the Langmuir isotherm model :

R_{ct} = {(R_{ct})}_{max} \cdot c \cdot \frac{K_{normala}}{1 + K_{a} c}

…”

Section: Resultsmentioning

confidence: 99%

Systematical investigation of binding interaction between novel ruthenium(II) arene complex with curcumin analogs and ctDNA

Huang

Yu²,

Huang

et al. 2016

Luminescence

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In this study, the interaction between a novel ruthenium(II) arene complex with curcumin analogs and calf thymus DNA (ctDNA) was investigated systematically by viscosity measurement, the DNA melting approach, multispectroscopic techniques and electrochemical methods. The absorption spectra of the ctDNA-drug complex showed a slight red shift and a weak hypochromic effect. The relative viscosity and melting temperature of ctDNA increased on addition of the drug. The evidence obtained from fluorescence competitive experiments indicated that the binding mode of the drug with ctDNA was intercalative. Using acridine orange (AO) as a fluorescence probe, the drug statically quenched the fluorescence of the ctDNA-AO complex, and hydrogen bonding and van der Waals interactions played vital roles in the binding interaction between the drug and ctDNA. The influences of ionic strength, chemical denaturants and pH on the binding interaction were also investigated. Circular dichroism and Fourier transform infrared spectra suggested that this drug might bond with the G-C base pairs of ctDNA and the right-handed B-form helicity of ctDNA remained after drug binding. The intercalative binding between the drug and ctDNA was further investigated using electrochemical techniques. All these results suggested that the biological activity of ctDNA was affected by ruthenium(II) arene complex with curcumin analogs. Copyright © 2016 John Wiley & Sons, Ltd.

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Study on the molecular interaction of graphene quantum dots with human serum albumin: Combined spectroscopic and electrochemical approaches

Cited by 108 publications

References 49 publications

Chronic exposure to graphene-based nanomaterials induces behavioral deficits and neural damage inCaenorhabditis elegans

Chronic exposure to graphene-based nanomaterials induces behavioral deficits and neural damage inCaenorhabditis elegans

Evaluation of interaction between imidazolium-based chloride ionic liquids and calf thymus DNA

Systematical investigation of binding interaction between novel ruthenium(II) arene complex with curcumin analogs and ctDNA

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