Systematical investigation of binding interaction between novel ruthenium(II) arene complex with curcumin analogs and ctDNA

Abstract

In this study, the interaction between a novel ruthenium(II) arene complex with curcumin analogs and calf thymus DNA (ctDNA) was investigated systematically by viscosity measurement, the DNA melting approach, multispectroscopic techniques and electrochemical methods. The absorption spectra of the ctDNA-drug complex showed a slight red shift and a weak hypochromic effect. The relative viscosity and melting temperature of ctDNA increased on addition of the drug. The evidence obtained from fluorescence competitive experiments indicated that the binding mode of the drug with ctDNA was intercalative. Using acridine orange (AO) as a fluorescence probe, the drug statically quenched the fluorescence of the ctDNA-AO complex, and hydrogen bonding and van der Waals interactions played vital roles in the binding interaction between the drug and ctDNA. The influences of ionic strength, chemical denaturants and pH on the binding interaction were also investigated. Circular dichroism and Fourier transform infrared spectra suggested that this drug might bond with the G-C base pairs of ctDNA and the right-handed B-form helicity of ctDNA remained after drug binding. The intercalative binding between the drug and ctDNA was further investigated using electrochemical techniques. All these results suggested that the biological activity of ctDNA was affected by ruthenium(II) arene complex with curcumin analogs. Copyright © 2016 John Wiley & Sons, Ltd.

Comparative study of binding interactions between three organometallic rhodium(III) complexes with curcuminoid ligands and human serum albumin

“…According to EIS is also another efficient method to calculate the binding constants of foreign substances with HSA on modified electrode surface. 44 In traditional EIS curve, the semicircular portion often means the electron-transfer-limited process and the diameter of this portion represents the charge transfer resistance (R ct ) of redox probe at electrode surface. 44 Usually, the R ct value will be changed with the variation of the electrode surface property.…”

“…44 In traditional EIS curve, the semicircular portion often means the electron-transfer-limited process and the diameter of this portion represents the charge transfer resistance (R ct ) of redox probe at electrode surface. 44 Usually, the R ct value will be changed with the variation of the electrode surface property. bly, the K a-EIS value of complex 3-HSA system is about three times higher than those of other two complex-HSA systems, so complex 3 exhibits the strongest binding ability with HSA.…”

Silver‐doped graphite carbon nitride nanosheets as fluorescent probe for the detection of curcumin

“…Curcumin is a chemical component extracted from plants of Zingiberacea and used as a traditional Chinese medicine . Medical studies have demonstrated that curcumin has many pharmacological effects, such as antioxidant, anticancer, anti‐inflammatory, and antibacterial . It is used in the treatment of rheumatism, Alzheimer's disease, diabetes, and drug‐resistant tuberculosis in clinic.…”

Molecular interaction studies of zinc sulphide nanoparticles with DNA and its consequence: a multitechnique approach

“…[ 1–7 ] Nanoparticles bind directly to the double helix of DNA through intercalative binding, groove binding, and electrostatic binding. [ 4,8–10 ] Intercalative mode of binding refers to nanoparticles π–π stacking interactions between adjacent base pairs of DNA resulting in structural perturbation of double helix. Nanoparticles interact with the edges of base pairs either major or minor grooves through noncovalent bonds are known as groove binding.…”

“…Similarly, coulombic interactions that occur between the negatively charged phosphate groups of DNA and the cationic portion of the nanoparticles are referred to as electrostatic binding. [ 4,8–10 ]…”