Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by <i>Trichinella spiralis</i> muscle larvae ESPs

Luo, Jingmei; Liu, Yu; Xie, Gary; Li, Dan; Shao, Meng; Zhao, Xin; Du, Lixin

doi:10.1017/s0031182016002535

Cited by 18 publications

(19 citation statements)

References 24 publications

(45 reference statements)

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“…Similar inhibitory effects were also found with the myeloma cell line SP2/0 [16]. In addition, T. spiralis muscle larvae (ML) ESP can induce apoptosis in small cell lung cancer H446 cells through the mitochondrial pathway in vitro [17].…”

Section: Introductionsupporting

confidence: 63%

Trichinella spiralis ESP inhibits tumor cell growth by regulating the immune response and inducing apoptosis

Ding¹,

Tang²,

Bai³

et al. 2021

Preprint

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Background Although Trichinella spiralis (T. spiralis) causes zoonotic diseases, it has a strong immunomodulatory effect and has therapeutic potential for various autoimmune diseases and cancers. Our previous study results showed that T. spiralis infection can inhibit the growth of liver cancer cells, but the specific mechanism has not been elucidated. Methods BALB/c mice injected with H22 cells and then infected with T. spiralis were used to detect tumor inhibition rate. Cell proliferation and apoptosis of H22 cells treated with excretory-secretory product (ESP) were measured by Cell-Counting Kit 8 (CCK-8) and Flow Cytometry (FCM). The expression of apoptosis-related genes in H22 cells and tumor tissues was detected by western blotting and real-time quantitative PCR (qPCR). IL-2, IFN-γ and IL-4 production in the spleens were measured by qPCR and enzyme-linked immunosorbent assay(ELISA). Results The growth of tumors in tumor model mice infected with T. spiralis was significantly inhibited compared with those uninfected tumor model mice. ESP could inhibit H22 cell proliferation and induce apoptosis through the mitochondrial pathway both in vitro and in vivo. Additionally, the levels of Th1 cytokines with antitumor effects were significantly increased in the early stage of T. spiralis infection, while Th2 cytokines increased later than Th1 cytokines. Conclusions ESP can directly induce tumor cell apoptosis and indirectly inhibit tumor cell growth through the host immune system, which is the potential antitumor mechanism of T. spiralis infection.

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Section: Introductionsupporting

confidence: 63%

Trichinella spiralis ESP inhibits tumor cell growth by regulating the immune response and inducing apoptosis

Ding¹,

Tang²,

Bai³

et al. 2021

Preprint

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show abstract

“…Trichinella spiralis muscle larva ES proteins inhibit the proliferation of human H446 small cell lung cancer cells and induce apoptosis by activating mitochondrial apoptosis pathways (Luo et al ., 2017). The A200711 protein of T. spiralis induces apoptosis in H7402 hepatocellular carcinoma cells (Wang et al ., 2013).…”

Section: Application Of T Spiralis or Its Products In Therapy Of Tummentioning

confidence: 99%

Trichinella spiralis:inflammation modulator

et al. 2020

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The hygiene hypothesis posits that the decreased incidence of parasitic infection in developed countries may underlie an increased prevalence of allergic and autoimmune diseases in these countries. As unique inflammation modulator of intracellular parasitism, Trichinella spiralis, or its excretory–secretory (ES) product, shows improved responses to allergies, autoimmune diseases, inflammatory bowel disease, type 1 diabetes, rheumatic arthritis and autoimmune encephalomyelitis by exerting immunomodulatory effects on both innate and adaptive immune cells in animal models. Research has shown that T. spiralis differs from other helminths in manipulation of the host immune response not only by well-known characteristics of its life cycle, but also by its inflammation modulation pathway. How the parasite achieves inflammation modulation has not been fully elucidated yet. This review will generalize the mechanism and focuses on ES immunomodulatory molecules of T. spiralis that may be important for developing new therapeutics for inflammatory disorders.

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“…Luo et al suggested that T. spiralis ML ESPs induce apoptosis in H446 cells through a mitochondrial pathway, which may represent the antitumor mechanism [24]. ML ESPs, as parasitic products that are released during the chronic phase of infection, reduce the survival and slightly, but significantly increase the apoptosis of melanoma cells in vitro [43].…”

Section: Discussionmentioning

confidence: 99%

“…The effect of T. spiralis on cells and molecules of the host's immune system is achieved through ESPs [34]. Trichinella spiralis ESPs contain antitumor-active substances that inhibit tumor growth [24,44,45]. ESPs of T. spiralis muscle larvae (ML) can induce the transformation of rat bone marrow-derived dendritic cells (BMDCs) to semi-matured status DCs [5].…”

Section: Introductionmentioning

confidence: 99%

Murine hepatoma treatment with mature dendritic cells stimulated by Trichinella spiralis excretory/secretory products

et al. 2020

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Excretory/Secretory Products (ESPs) of the nematode Trichinella spiralis contain antitumor-active substances that inhibit tumor growth. Mature dendritic cells (DCs) play a critical role in the antitumor immunity of the organism. As pathogen-derived products, it ought to be discussed whether T. spiralis ESPs will reduce the antitumor effect of mature DCs from the host before it is applied to patients’ tumors. Therefore, the aim of this work was to evaluate the immunological effect of DCs stimulated by T. spiralis ESPs in H22 tumor-bearing mice. H22 tumor model mice in this study were randomly divided into four groups according to the treatment: PBS control group, ESP group, DCs group, and DCs stimulated with T. spiralis ESP (ESP+DCs group). The antitumor effect was evaluated by tumor inhibition rate and cytokine detection using ELISA. The results showed significant inhibition in tumor growth in the ESP+DCs, DCs and ESP groups when compared with the PBS control group (p < 0.01, p < 0.01, and p < 0.05, respectively). However, no significant difference was observed on tumor inhibition rates between the ESP+DCs and DCs groups. The decrease in IL-4, IL-6, and IL-10, and the increase in IFN-γ between the DCs and ESP+DCs groups were also not significant. Therefore, DCs stimulated by ESP did not reduce the antitumor effect of mature DCs, which demonstrated that the T. spiralis ESP would not affect the antitumor effect of mature DCs by modulating the immune response of the host, and that ESPs are safe in antitumor immunology when applied in a tumor model mice.

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Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by Trichinella spiralis muscle larvae ESPs

Cited by 18 publications

References 24 publications

Trichinella spiralis ESP inhibits tumor cell growth by regulating the immune response and inducing apoptosis

Trichinella spiralis ESP inhibits tumor cell growth by regulating the immune response and inducing apoptosis

Trichinella spiralis:inflammation modulator

Murine hepatoma treatment with mature dendritic cells stimulated by Trichinella spiralis excretory/secretory products

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