Study on the mechanism of Salvia miltiorrhiza polysaccharides in relieving liver injury of broilers induced by florfenicol

Abstract

In order to explore the transcriptomics and proteomics targets and pathways of Salvia miltiorrhiza polysaccharides (SMPs) alleviating orfenicol (FFC)-induced liver injury in broilers,60 1-day-old broilers were randomly divided into 3 groups: control group ( GP1) was fed tap water, FFC model (GP2) was given tap water containing FFC 0.15 g/L, and SMPs treatment group (GP3) was given tap water containing FFC 0.15 g/L and SMPs 5 g/L.Starting from 1 day of age, the drug was administered continuously for 5 days. On the 6th day, blood was collected from the heart and the liver was taken. Then 3 chickens were randomly taken from each group, and their liver tissues were aseptically removed and placed in an enzyme-free tube. Using high-throughput mRNA sequencing and TMT-labeled quantitative proteomics technology, the transcriptome and proteome of the three groups of broiler liver were analyzed respectively. The results of the study showed that the liver tissue morphology of the chicks in the GP1 and GP3 groups was complete, and there were no obvious necrotic cells in the liver cells. The liver tissue cells in the GP2 group showed obvious damage, the intercellular space increased, and the liver cells showed extensive vacuolation and steatosis. Compared with the GP1 group, the daily gain of chicks in the GP2 group was signi cantly reduced (P < 0.0 5 or P < 0.01). Compared with the GP2 group, the GP3 group signi cantly increased the daily gain of chicks (P <0.0 5 or P <0.01). Compared with the GP1 group, the serum levels of ALT, AST, liver LPO, ROS and IL-6 in the GP2 group were signi cantly increased (P < 0.0 5 or P < 0.01), and the contents of T-AOC, GSH-PX, IL-4 and IL-10 in the liver were signi cantly decreased (P < 0.0 5 o r P < 0.01). After SMPs treatment, the serum levels of ALT, AST, liver LPO, ROS and IL-6 were signi cantly reduced (P < 0.0 5 or P < 0.01), and the contents of T-AOC, GSH-PX, IL-4 and IL-10 in the liver were signi cantly increased (P < 0.0 5 or P < 0.01). There were 380 mRNA and 178 protein differentially expressed between GP2 group and GP3 group. Part of DEGs was randomly selected for QPCR veri cation, and the expression results of randomly selected FABP1, SLC16A1, GPT2, AACS and other genes were veri ed by QPCR to be consistent with the sequencing results, which demonstrated the accuracy of transcriptation-associated proteomics sequencing. The results showed that SMPs could alleviate the oxidative stress and in ammatory damage caused by FFC in the liver of chicken and restore the normal function of the liver. SMPs may alleviate the liver damage caused by FFC by regulating the drug metabolism -cytopigment P450, PPAR signaling pathway, MAPK signaling pathway, glutathione metabolism and other pathways.

Recent developments in Salvia miltiorrhiza polysaccharides: Isolation, purification, structural characteristics and biological activities

“…However, studies have found that FFC could promote oxidative stress and accelerate hepatocyte apoptosis, thus affecting the liver function of chickens (Han et al, 2020;Liu et al, 2022). In a model of liver injury caused by FFC in broilers, SMP alleviates oxidative stress and inflammatory injury in the liver, improves liver function and plays a liver protective role, which provides theoretical support for the study of SMP on the treatment of drug-induced liver injury (Geng et al, 2022). Endotoxin metabolism activates the Toll-like receptor 4 (TLR4) pathway in the liver, leading to the increased expression of inflammatory cytokines and chemokines and then to liver inflammation.…”

Research progress on the mechanisms underlying poultry immune regulation by plant polysaccharides

“…In a poultry hepatocyte injury model, SMPs can alleviate liver injury and metabolic disorders through drug metabolism induced by cytochrome P450 and the glycine, serine, and threonine metabolism signaling pathways ( 114 ). Through transcriptome and proteome studies, Geng et al ( 115 ) found that SMPs alleviate oxidative stress and injury in broiler livers through the PPAR signaling pathway, MAPK signaling pathway, and glutathione metabolism ( 115 ). After poultry liver injury, the contents of total protein (TP), albumin (Alb), and GSH increased significantly, while after SMPs treatment, the contents of ALT, AST, and MDA decreased significantly, indicating that SMPs have a good protective effect on poultry liver injury in vivo and in vitro ( 115 , 116 ).…”

“…Through transcriptome and proteome studies, Geng et al ( 115 ) found that SMPs alleviate oxidative stress and injury in broiler livers through the PPAR signaling pathway, MAPK signaling pathway, and glutathione metabolism ( 115 ). After poultry liver injury, the contents of total protein (TP), albumin (Alb), and GSH increased significantly, while after SMPs treatment, the contents of ALT, AST, and MDA decreased significantly, indicating that SMPs have a good protective effect on poultry liver injury in vivo and in vitro ( 115 , 116 ). In addition, the strength of immunity is closely related to that of the kidneys.…”

Salvia miltiorrhiza polysaccharides alleviates florfenicol-induced liver metabolic disorder in chicks by regulating drug and amino acid metabolic signaling pathways

“…Our previous studies found that SMPs may affect lipid metabolism, inflammation, antioxidant and drug metabolism pathways in livers of chicks at the overall biological function level ( Han et al, 2021 ; Geng et al, 2022 ). However, the previous studies were not in-depth and did not clearly indicate the directional regulation of SMPs on specific signaling pathways and the interaction between genes and proteins in the changed signaling pathways.…”