Study on the Inhibitory Effect of Tannins and Flavonoids against the 1,1-Diphenyl-2-picrylhydrazyl Radical

Yokozawa, Takako; Chen, Cui Ping; Dong, Erbo; Tanaka, Takashi; Nonaka, Gen‐ichiro; Nishioka, Itsuo

doi:10.1016/s0006-2952(98)00128-2

Cited by 551 publications

(394 citation statements)

References 38 publications

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“…[4][5][6][7][8][9][10] The antioxidant activity of phenolic compounds is inuenced by the number and position of phenolic hydroxyls and other substituents, and glycosylation of the molecules. 11,12 Furthermore, antitumor properties have also been attributed to different phenolic compounds, including avones. 13 Other important antioxidant molecules are tocopherols, which are considered as one of the most important antioxidants to combat oxidative stress, because they inhibit the production of peroxyl radicals, protecting cells of oxidative damage to low density lipoproteins, proteins and DNA, and of membrane degeneration due to peroxidation of polyunsaturated fatty acids.…”

Section: Introductionmentioning

confidence: 99%

Bioactivity and phytochemical characterization of Arenaria montana L.

et al. 2014

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The bioactivity (antioxidant and cytotoxic activities) of the aqueous and methanolic extracts of Arenaria montana L., a plant commonly used in Portuguese folk medicine, was evaluated and compared.Furthermore, the phytochemical composition was determined based on hydrophilic (sugars, organic acids and phenolic compounds) and lipophilic (fatty acids and tocopherols) compounds, in order to valorize this plant material as a functional food/nutraceutical. Fructose, oxalic acid, methyl-luteolin 2 00 -O-feruloylhexosyl-C-hexoside, a-tocopherol, and linoleic acid were the main individual compounds found in A. montana. In general, the aqueous extract showed higher antioxidant and cytotoxic activities than the methanolic extract; the latter showed activity only against HeLa and HepG2 cell lines. Both aqueous and methanolic extracts showed some hepatotoxicity but at higher doses than the ones active for tumor cell lines. Moreover, the aqueous extract of A. montana may be used as a functional food or nutraceutical due to the high antioxidant and cytotoxic activities, and due to the presence of bioactive compounds. As far as we know, this is the first report on the phytochemical composition and bioactivity of A. montana.

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Section: Introductionmentioning

confidence: 99%

Bioactivity and phytochemical characterization of Arenaria montana L.

et al. 2014

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“…This procedure is fast and, at the same time, sensitive (Yokozawa et al, 1998). Hydrogen donor molecules present in the extract that react with the DPPH radical cause a decrease in the intensity of absorption at 517 nm and can help identify extracts with anti-radical compounds.…”

Section: Resultsmentioning

confidence: 99%

“…DPPH (1,1-diphenyl-2-picrylhydrazyl) solution were prepared in methanol, as previously published (Yokozawa et al, 1998;Pellat et al, 2004). In a 96-well microplate, 10 μL of a 1 mM solution of DPPH and appropriate volumes of antioxidant (to obtain final concentrations of 0.1, 0.5, 1.0, 1.5 and 2.0 mg/mL) were added and the final volume of 100 μL completed with methanol.…”

Section: Antioxidant Activitymentioning

confidence: 99%

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Antioxidant activity and chemical composition of the non polar fraction of Gracilaria domingensis (Kützing) Sonder ex Dickie and Gracilaria birdiae (Plastino & Oliveira)

Guaratini

Lopes

Marinho‐Soriano³

et al. 2012

Rev. bras. farmacogn.

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Gracilaria domingensis (Kützing) Sonder ex Dickie and Gracilaria birdiae (Plastino & Oliveira) (Gracilariales, Rhodophyta) are seaweeds that occur on the Brazilian coast. Based on their economic and pharmaceutical importance, we investigated the antioxidant activity of the methanolic, ethyl acetate and hexane extracts of both species. The hexane extracts display a high antioxidant activity and comparative analyses indicated G. birdiae as the most active species. Chemical investigation of these fractions showed several carotenoids and fatty acids, as well as cholesterol and sitosterol derivatives. HPLC-DAD analysis of G. birdiae showed violaxanthin (0.04 μg.mg -1 of dry material), antheraxanthin (5.31 μg.mg -1 ), aloxanthin (0.09 μg.mg -1 ), zeaxanthin (0.45 μg.mg -1 ) and β-carotene (0.37 μg.mg -1 ) as the major carotenoids. G. domingensis showed a similar carotenoid profi le, however, with much lower concentration than G. birdiae. Gas chromatography coupled to mass spectrometry was used to determine other nonpolar compounds of these seaweeds. The main compounds detected in both studied species were the fatty acids 16:0; 18:1 Δ 9 ; 20:3 Δ 6,9,12 , 20:4 Δ 5,8,11,14 . We found no specifi city of compounds in either species. However, G. birdiae, presented higher contents of carotenoids and arachidonic acid than G. domingensis.

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“…These compounds had a high DPPH scavenging activity, such as quercetin (IC 50 = 0.83 µg/mL) and kaempferol (IC 50 = 1.87 µg/mL) (Cho et al, 2003). Almost all tannin compounds had been studied to show high DPPH scavenging activity in a small concentration range [24]. The compound content caused the matoa leaves and stem bark extracts to have very strong DPPH scavenging activity, but both extracts did not have good tyrosinase inhibitory ability.…”

Section: Antioxidant Activity Test Of the Matoa Stem Bark Extract Framentioning

confidence: 99%

Tyrosinase Inhibition, 2,2-Diphenyl-1-Picrylhydrazyl Radical Scavenging Activity, and Phytochemical Screening of Fractions and Ethanol Extract From Leaves and Stem Bark of Matoa (Pometia Pinnata)

Sauriasari

Azizah

Basah

2017

Asian J Pharm Clin Res

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Objective: This study aims to investigate the potency of matoa as a tyrosinase inhibitor and antioxidant and also to identify the chemical compounds in the most active fraction and an ethanol extract from the leaves and stem bark of matoa.Methods: The extracts were tested for their tyrosinase inhibitory activity by evaluating the formation of L-dopachrome at 490 nm. Antioxidant activity was tested using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The most active extract from liquid-liquid partition analysis was fractionated, and then, the assays were performed. Results:The ethanol extract of leaves and stem bark of matoa showed weak anti-tyrosinase activity (percent inhibition was 24.54±0.22% and 21.93±0.57%, respectively, final concentration 200 μg/mL), but they showed strong DPPH radical scavenging activity (IC 50 values were 6.11 μg/mL and 5.47 μg/mL, respectively). The ethyl acetate fraction was the most active fraction with an IC 50 value of 5.38 μg/mL. Ethanol extract from the leaves and stem bark of matoa and the ethyl acetate fraction contains flavonoids, tannins, saponins, triterpenoids, and glycosides. Conclusion:Matoa does not have potency as a tyrosinase inhibitor, but it has good antioxidant activity, although still lower than that of quercetin.

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Study on the Inhibitory Effect of Tannins and Flavonoids against the 1,1-Diphenyl-2-picrylhydrazyl Radical

Cited by 551 publications

References 38 publications

Bioactivity and phytochemical characterization of Arenaria montana L.

Bioactivity and phytochemical characterization of Arenaria montana L.

Antioxidant activity and chemical composition of the non polar fraction of Gracilaria domingensis (Kützing) Sonder ex Dickie and Gracilaria birdiae (Plastino & Oliveira)

Tyrosinase Inhibition, 2,2-Diphenyl-1-Picrylhydrazyl Radical Scavenging Activity, and Phytochemical Screening of Fractions and Ethanol Extract From Leaves and Stem Bark of Matoa (Pometia Pinnata)

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