Study on the influence of adiponectin genetic variants and adiponectin levels among Indonesian women with polycystic ovary syndrome

Abstract

Latar belakang: Resistensi insulin dan obesitas sentral adalah keadaan yang sering ditemukan pada wanita PCOS dan ditandai dengan abnormalitas penanda biologi yang terkait dengan terjadinya gangguan metabolik. Hubungan antara adiponektin dan resistensi insulin telah banyak diteliti, namun penelitian terhadap pasien PCOS baru sedikit yang dilakukan. Penelitian ini bertujuan untuk menentukan kemungkinan hubungan polimorfisme T45G dengan penanda biologi PCOS dan pengaruhnya terhadap adiponektin serum pada populasi Indonesia. Metode: Lima puluh dua pasien PCOS dan 52 subjek ovulasi normal tanpa hiperandrogenisme sebagai kontrol disertakan dalam penelitian ini. Sampel darah dikumpulkan antara hari ke 3 dan 5 siklus menstruasi spontan, jam 7 hingga 9 pagi, setelah menjalani puasa. Dilakukan pengukuran kadar serum FSH, LH, testosteron, SHBG, glukosa, insulin, profil lipid dan adiponektin. Resistensi insulin ditentukan dengan HOMA-IR, HOMA-β, dan SHBG. DNA genom dari darah perifer pasien dan subjek kontrol digunakan untuk memeriksa polimorfisme T45G menggunakan metode PCR. Hasil: Terdapat perbedaan yang signifikan antara kelompok PCOS dan kontrol terhadap IMT, LH, testosteron, SHBG, dan FAI, tetapi tidak signifikan terhadap frekuensi distribusi polimorfisme gen T45G. Kadar adiponektin ditemukan lebih rendah pada kelompok PCOS daripada kontrol, dan terdapat hubungan antara resistensi insulin dengan PCOS. Pada pasien PCOS frekuensi polimorfisme T45G ditemukan lebih tinggi pada wanita dengan adiponektin kadar rendah dari pada kelompok adiponektin kadar tinggi, meskipun tidak bermakna secara statistik. Tidak ditemukan hubungan antara penanda biologi PCOS (LH, testosteron, SHBG, dan FAI) dengan polimorfisme gen T45G.

Sex hormone binding globulin - an important biomarker for predicting PCOS risk: A systematic review and meta-analysis

“…A total of 39 observational cross sectional, prospective cohort, and case control studies involving 5,121 patients and 5,059 controls were included. Of all the studies, 18 were performed in Europe [Dadachanji et al 2015;Lauritsen et al 2014;Schweighofer et al 2012;Kim et al 2012;Pangaribuan et al 2012;Schüring et al 2012;Houjeghani et al 2012;Koiou et al 2011;Tarkun et al 2004;Liu et al 2008;Li et al 2006;Sieminska et al 2004;Richard 2002;Lin et al 2015;Jayagopal 2003;Elci et al 2016;Kim et al 2013;Danilowicz et al 2014], 18 in Asia [Zhu et al 2016;Jamil et al 2015;Baldani et al 2015;O'Reilly et al 2014;Martínez-García et al 2012;Alili and Idrizi 2014;El-Shal et al 2013;Skrgatic et al 2012;Dikmen 2011;Chae et al 2010;Oh et al 2009;Xita et al 2008;Bendlov et al 2007;Atamer et al 2008;Ferk et al 2007, Tarkun et al 2010Anttila et al 1996;Prelević 1993], 2 in Africa [Song et al 2014;Yasmin et al 2013], and 1 in North America [Kim et al 2014]. Studies used a variety of methods to identify patients with PCOS.…”

“…Five studies statistically found no significant differences in the mean SHBG levels [Zhu et al 2016;Schweighofer et al 2012;Dikmen 2011;Atamer et al 2008;Ferk et al 2007]. According to the quality scoring assessment checklist, 8 studies having a score of more than 8 were considered high quality studies [Zhu et al 2016;Jamil et al 2015;Baldani et al 2015, Alili andIdrizi 2014;Pangaribuan et al 2012;Dikmen 2011;Koiou et al 2011;Tarkun et al 2010].…”

Clinical Impact of Insulin Resistance in Women with Polycystic Ovary Syndrome

“…Pangaribuan et al [141] also did not find significant difference in serum resistin levels between PCOS women and controls. Meanwhile, the authors did not find significant correlation between resistin, BMI and HOMA-index [141]. Similar serum resistin levels in normal-weight women with or without PCOS were found by Seow et al [142].…”

“…Resistin did not correlate with any hormonal or metabolite index in our Bulgarian population of PCOS women with overweight [135]. Pangaribuan et al [141] also did not find significant difference in serum resistin levels between PCOS women and controls. Meanwhile, the authors did not find significant correlation between resistin, BMI and HOMA-index [141].…”

Effect and Mechanism of Traditional Chinese Medicine Huolou Gukang Pill Combined With Simvastatin on Steroid-Induced Necrosis of Femoral Head

“…TNF-α is likely to be the main cause of hormonal necrosis of femoral head. Adiponectin has a strong biological role, can inhibit TNF-α and reduce the level of inflammatory factors in joints, thereby inhibiting the destruction of inflammation on joints, which plays an interfering role in occurrence of steroid-induced femoral head necrosis 15 . This study found that, compared with the control group, adiponectin of patients in study group with adiponectin increased significantly, but TNF-α, blood lipids and blood glucose decreased significantly.…”