Study on the fate of petroleum-derived polycyclic aromatic hydrocarbons (PAHs) and the effect of chemical dispersant using an enclosed ecosystem, mesocosm

Yamada, Mihoko; Takada, Hideshige; Toyoda, Kazuhiro; Yoshida, Akihiro; Shibata, Akira; Nomura, Hideaki; Wada, Mitsuru; Nishimura, Masahiko; Okamoto, Ken; Ohwada, Kenji

doi:10.1016/s0025-326x(03)00102-4

Cited by 117 publications

(48 citation statements)

References 23 publications

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“…The position of the arrow corresponds to the melting behaviour of isolate #27, classified as an uncultured Pseudomonas sp. (Shiah & Ducklow 1995), the high concentration of low-molecular-weight PAHs, generally easily degraded (Yamada et al 2003), at sampling Stn S3 can be related to the high relative abundance of Gammaproteobacteria at this site. Exposure to hydrocarbons has been shown to decrease bacterial diversity, as a result of selection in favour of PAH-degrading bacteria (Röling et al 2002, Castle et al 2006.…”

Section: The Effect Of Sampling Site On the Community Structure Of Thmentioning

confidence: 85%

Exploring hydrocarbonoclastic bacterial communities in the estuarine surface microlayer

Coelho¹,

Sousa²,

Santos³

et al. 2011

Aquat. Microb. Ecol.

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Bacteria that degrade polycyclic aromatic hydrocarbons (PAHs) in the estuarine surface microlayer (SML) of the Ria de Aveiro, Portugal -which is chronically polluted with oil hydrocarbons (OH) -were isolated and characterized; Pseudomonas was dominant among the PAH-degrading bacteria. Screening for PAH dioxygenase genes detected almost identical nahAc genes (encoding the alpha subunits of naphthalene dioxygenase) in 2 phylogenetically distinct isolates: Pseudomonas sp. and an unknown species of the family Enterobacteriaceae; this suggested that horizontal transfer of nah genes might be involved in PAH degradation in the SML. We also investigated the effect of PAH contamination on the spatial variability of the bacterioneuston along a gradient of pollution in the estuarine system of the Ria de Aveiro. Culture-independent techniques -fluorescence in situ hybridization (FISH) and denaturing-gradient gel electrophoresis (DGGE) -revealed a similar structure among the bacterioneuston communities along the estuary. In contrast, we detected differences in the relative abundance and diversity of organisms of the Gammaproteobacteria, including those of the genus Pseudomonas (which belongs to the Gammaproteobacteria). This is the first insight into the hydrocarbonoclastic bacterial communities in the SML of an estuarine area polluted with hydrocarbons. Our findings highlight the importance of SML-adapted hydrocarbonoclastic bacterioneuston as a potential source of new PAH-degrading bacteria (including new pseudomonads) with potential use in the bioremediation of hydrocarbon-polluted ecosystems. KEY WORDS: Sea surface microlayer · Bacterioneuston · Polycyclic aromatic hydrocarbons · PAH degradationResale or republication not permitted without written consent of the publisher

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Section: The Effect Of Sampling Site On the Community Structure Of Thmentioning

confidence: 85%

Exploring hydrocarbonoclastic bacterial communities in the estuarine surface microlayer

Coelho¹,

Sousa²,

Santos³

et al. 2011

Aquat. Microb. Ecol.

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“…It is also known that incorporation of PAHs in fecal pellets is important for vertical transport of PAHs owing to rapid sinking and prevention from chemical and biological degradation (Lipiatou et al 1993;Takada et al 1994). Although it is difficult to elucidate a major mechanism for the disappearance of Pyre and Flth from the water body, we considered that incorporation in fecal pellets (Lipiatou et al 1993;Takada et al 1994), absorption in sinking particles (Yamada et al 2003), or both, should be significant. At Monthly changes in other indicator molecules in the sediment trap samples…”

Section: Fate Of Pyre and Flth In Lake Biwamentioning

confidence: 99%

Elucidation of polycyclic aromatic hydrocarbon sources in the sinking particles in Lake Biwa, Japan

et al. 2010

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“…However, WSF addition also resulted in increasing bacterial abundance followed by a decrease at the end of the experiment. This decrease could be ascribable to grazing by heterotrophic flagellates: dissolved hydrocarbons as an additional carbon supply supported an increase in the number of bacteria, which in turn probably accelerated the growth of bacterial grazers (Yamada et al 2003).…”

Section: Mechanisms and Relevance Of Wsf Addition To Plankton Assemblagementioning

confidence: 99%

Non-synergistic effects of water-soluble crude oil and enhanced ultraviolet-B radiation on a natural plankton assemblage

Sargian¹,

Mostajir²,

Chatila³

et al. 2005

Mar. Ecol. Prog. Ser.

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The present study demonstrates the effects of the water-soluble fraction (WSF) of a crude oil, enhanced ultraviolet-B radiation (UVBR: 280 to 320 nm), and the combination of WSF and enhanced UVBR on a natural plankton assemblage (<150 μm) isolated from the lower St. Lawrence Estuary. To study the separate and dual effects of WSF and UVBR, 12 microcosms (9 l) were immersed in the water column of larger mesocosms (polyethylene bags; 1800 l), providing 4 treatments, each in triplicate: (1) NUVBR + WSF (natural UVBR with WSF), (2) HUVBR + WSF (enhanced UVBR with WSF), (3) NUVBR (natural UVBR without WSF), and (4) HUVBR (enhanced UVBR without WSF). During 5 d we monitored the incident radiation, WSF and nutrient concentrations, abundance and production of heterotrophic bacteria and phytoplankton. Strong deleterious effects of WSF and lower effects of UVBR were observed on the phytoplankton assemblage, with a decrease in growth rates accompanied by an increase in mean cell size which reflected a perturbation of the cell division cycle. Using the NUVBR treatement as reference conditions, the above effects resulted in a reduction of 84, 79 and 60% of total abundance of the phytoplankton fraction < 20 μm in the HUVBR + WSF, NUVBR + WSF and HUVBR treatments, respectively. Significant higher values of bacterial abundances were observed in the WSF-added treatments compared to NUVBR without WSF. However, bacterial thymidine incorporation exhibited diel variations, suggesting cumulative UVBR-induced DNA and/or PAHinduced DNA damages, and possible repair mechanisms with the co-occurrence of more available growth substrates from stressed phytoplankton. The absence of significant differences between both WSF-added treatments under the 2 different UVBR conditions suggests that there is no additive interaction between WSF and UVBR. This study provides therefore the first evidence of a non-synergistic interaction between both stresses, and suggests that UVBR-induced effects on marine microorganisms can be completely masked by the strong deleterious effects of soluble petroleum hydrocarbons.KEY WORDS: Ultraviolet radiation · Dissolved hydrocarbons · Non-synergistic effect · Phytoplankton · BacteriaResale or republication not permitted without written consent of the publisher

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Study on the fate of petroleum-derived polycyclic aromatic hydrocarbons (PAHs) and the effect of chemical dispersant using an enclosed ecosystem, mesocosm

Cited by 117 publications

References 23 publications

Exploring hydrocarbonoclastic bacterial communities in the estuarine surface microlayer

Exploring hydrocarbonoclastic bacterial communities in the estuarine surface microlayer

Elucidation of polycyclic aromatic hydrocarbon sources in the sinking particles in Lake Biwa, Japan

Non-synergistic effects of water-soluble crude oil and enhanced ultraviolet-B radiation on a natural plankton assemblage

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Study on the fate of petroleum-derived polycyclic aromatic hydrocarbons (PAHs) and the effect of chemical dispersant using an enclosed ecosystem, mesocosm

Cited by 117 publications

References 23 publications

Exploring hydrocarbonoclastic bacterial ­communities in the estuarine surface microlayer

Exploring hydrocarbonoclastic bacterial ­communities in the estuarine surface microlayer

Elucidation of polycyclic aromatic hydrocarbon sources in the sinking particles in Lake Biwa, Japan

Non-synergistic effects of water-soluble crude oil and enhanced ultraviolet-B radiation on a natural plankton assemblage

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Exploring hydrocarbonoclastic bacterial communities in the estuarine surface microlayer

Exploring hydrocarbonoclastic bacterial communities in the estuarine surface microlayer