Study on the extraction, purification and quantification of jasmonic acid, abscisic acid and indole‐3‐acetic acid in plants

Zhang, Feng Juan; Jin, You Ju; Xu, Xiangxing; Lu, Rong; Chen, Hua Jun

doi:10.1002/pca.1085

Cited by 41 publications

(30 citation statements)

References 24 publications

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“…Quantitative determination of ABA content was performed following the protocol described by [80] with minor modifications. Briefly, frozen fruit (5 g) was homogenised in cold (4°C) 80% methanol containing 100 µg butylated hydroxytoluene, and 324 ng D 3 -ABA (Roger Horgan, Garden City, NY, USA) as the internal standard.…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of a Strawberry FaASR Gene in Relation to Fruit Ripening

et al. 2011

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BackgroundABA-, stress- and ripening-induced (ASR) proteins have been reported to act as a downstream component involved in ABA signal transduction. Although much attention has been paid to the roles of ASR in plant development and stress responses, the mechanisms by which ABA regulate fruit ripening at the molecular level are not fully understood. In the present work, a strawberry ASR gene was isolated and characterized (FaASR), and a polyclonal antibody against FaASR protein was prepared. Furthermore, the effects of ABA, applied to two different developmental stages of strawberry, on fruit ripening and the expression of FaASR at transcriptional and translational levels were investigated.Methodology/Principal Findings FaASR, localized in the cytoplasm and nucleus, contained 193 amino acids and shared common features with other plant ASRs. It also functioned as a transcriptional activator in yeast with trans-activation activity in the N-terminus. During strawberry fruit development, endogenous ABA content, levels of FaASR mRNA and protein increased significantly at the initiation of ripening at a white (W) fruit developmental stage. More importantly, application of exogenous ABA to large green (LG) fruit and W fruit markedly increased endogenous ABA content, accelerated fruit ripening, and greatly enhanced the expression of FaASR transcripts and the accumulation of FaASR protein simultaneously.ConclusionsThese results indicate that FaASR may be involved in strawberry fruit ripening. The observed increase in endogenous ABA content, and enhanced FaASR expression at transcriptional and translational levels in response to ABA treatment might partially contribute to the acceleration of strawberry fruit ripening.

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Section: Methodsmentioning

confidence: 99%

Molecular Characterization of a Strawberry FaASR Gene in Relation to Fruit Ripening

et al. 2011

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“…Additionally, jasmonic acid and methyl jasmonate possess good solubility in polar solvents, which is difficult to eliminate large amounts of polar compounds that coexist. Solid-phase extraction (SPE) emerged to simplify the labor-intensive liquid-liquid extraction (Thurman and Snavely 2000) and has been widely used in pretreatment of different kinds of plant hormones (Ma et al 2008;Zhang et al 2008;Blanch et al 2009;X. Liu et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Polymer Monolith Microextraction Coupled with HPLC for Determination of Jasmonates in Wintersweet Flowers

Zhang

Chen

2013

Analytical Letters

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A simple, fast, and effective method has been presented for the determination of jasmonates in plant samples by polymer monolith microextraction (PMME). A poly (methacrylic acid-ethylene glycol dimethacrylate) (MAA-EGDMA) monolith-based device was developed for extraction, purification, and concentration; HPLC-UV was used for evaluation. To realize the best microextraction efficiency, parameters such as sample pH value, flow rate, and sample volume were systematically examined and optimized. Aqueous solution (5 mL) of jasmonates at pH 3.0 was selected as sample solution, and loaded onto the monolith at flow rate of 0.15 mL/min; finally, 50 lL of acetonitrile was used for elution. The proposed method exhibited impressive enrichment efficiency (almost 100-fold) and the limits of detection for jasmonic acid and methyl jasmonate obtained 0.5 and 2 ng/mL by using UV detection. Wide linear ranges were also observed (2-2000 and 5-2000 ng/mL) for both jasmonic acid and methyl jasmonate, with R 2 > 0.999. The developed PMME-HPLC method was successfully applied to the determination of jasmonates in fresh wintersweet flowers with recoveries in the range of 91.9-97.2%. The result was confirmed by an HPLC-MS method. The PMME method was also compared with a conventional C18-SPE method and exhibited better clean-up efficiency.

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“…SIM provides higher sensitivity and selectivity than monitoring all ions simultaneously, a data acquisition mode named full scan. Chemical ionization and electron ionization (EI) are two widely used ionization methods for GC-MS analysis [13,16,74,75]. However, EI typically uses electrons at 70 eV, which yields more fragments ions.…”

Section: Chromatography and Chromatography/mass Spectrometrymentioning

confidence: 99%

“…The major classes of sorbents in use are summarized in Table 2. The main separation modes used are ion-exchange [34,35], reversed-phase (C 18 , [16,[36][37][38][39][40]; Oasis HLB, [8,28,41]) and mixed mode (Oasis MAX, [41]; Oasis MCX, [28,39,40]). Considering the complex interference compounds that might be present in the plant extracts, more than one kind of sorbent is used to enrich the plant hormones efficiently.…”

Section: Purificationmentioning

confidence: 99%

Progress in quantitative analysis of plant hormones

Sun

Wang

et al. 2011

Chin. Sci. Bull.

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Plant hormones are small molecular natural products that regulate all plant developmental processes at low concentrations. Quantitative analysis of plant hormones is increasingly important for in-depth study of their biosynthesis, transport, metabolism and molecular regulatory mechanisms. Although plant hormone analysis remains a bottleneck in plant scientific research owing to the trace concentrations and complex components in plant crude extracts, much progress has been achieved in the development of extraction, purification and detection techniques in recent years. Solid phase extraction and chromatography/mass spectrometry have been applied widely for purification and quantitative analysis of plant hormones owing to their high selectivity and sensitivity. Purification methods such as liquid partition and immunoaffinity chromatography, and detection methods including immunoassay and electrochemical analysis, are employed. The advantages and disadvantages of these methods are discussed. In situ, real-time and multi-plant hormone profiling will comprise mainstream techniques for quantitative analyses in future studies on the regulatory mechanisms and crosstalk of plant hormones. defense responses are all triggered by plant hormones. A plant hormone, acting as a plant-development controller or a plant-environment mediator, can influence crop yield directly or indirectly, therefore research into plant hormone physiology has become an important target for agriculture development. However, it is noteworthy that endogenous level of each plant hormone is very low [1], and quantitative analysis of these compounds is still extremely difficult for both biologists and analytical chemists. It is a challenge for researchers to enrich these low-level natural compounds from plant extracts in which large amounts of interfering substances are present. In addition, the limited quantities of plant tissue available for hormone quantification is another challenge. Consequently, the analytic methods must be extremely selective and sensitive. The quantitative analysis of plant hormones typically includes the following key points: (i) the plant materials must

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Study on the extraction, purification and quantification of jasmonic acid, abscisic acid and indole‐3‐acetic acid in plants

Abstract: A simple, inexpensive method was developed for determining endogenous IAA, ABA and JA concentrations in plant tissue.

Cited by 41 publications

References 24 publications

Molecular Characterization of a Strawberry FaASR Gene in Relation to Fruit Ripening

Molecular Characterization of a Strawberry FaASR Gene in Relation to Fruit Ripening

Polymer Monolith Microextraction Coupled with HPLC for Determination of Jasmonates in Wintersweet Flowers

Progress in quantitative analysis of plant hormones

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