Progress in quantitative analysis of plant hormones

Abstract

Plant hormones are small molecular natural products that regulate all plant developmental processes at low concentrations. Quantitative analysis of plant hormones is increasingly important for in-depth study of their biosynthesis, transport, metabolism and molecular regulatory mechanisms. Although plant hormone analysis remains a bottleneck in plant scientific research owing to the trace concentrations and complex components in plant crude extracts, much progress has been achieved in the development of extraction, purification and detection techniques in recent years. Solid phase extraction and chromatography/mass spectrometry have been applied widely for purification and quantitative analysis of plant hormones owing to their high selectivity and sensitivity. Purification methods such as liquid partition and immunoaffinity chromatography, and detection methods including immunoassay and electrochemical analysis, are employed. The advantages and disadvantages of these methods are discussed. In situ, real-time and multi-plant hormone profiling will comprise mainstream techniques for quantitative analyses in future studies on the regulatory mechanisms and crosstalk of plant hormones. defense responses are all triggered by plant hormones. A plant hormone, acting as a plant-development controller or a plant-environment mediator, can influence crop yield directly or indirectly, therefore research into plant hormone physiology has become an important target for agriculture development. However, it is noteworthy that endogenous level of each plant hormone is very low [1], and quantitative analysis of these compounds is still extremely difficult for both biologists and analytical chemists. It is a challenge for researchers to enrich these low-level natural compounds from plant extracts in which large amounts of interfering substances are present. In addition, the limited quantities of plant tissue available for hormone quantification is another challenge. Consequently, the analytic methods must be extremely selective and sensitive. The quantitative analysis of plant hormones typically includes the following key points: (i) the plant materials must

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

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