Study on the Diagnosis of Gastric Cancer by Magnetic Beads Extraction and Mass Spectrometry

Abstract

Objective. This study constructed a model for the early diagnosis of gastric cancer by comparing the serum peptides profiles of patients with advanced gastric cancer and healthy people. And that model may be the potential to be applied for the efficacy evaluation and recurrence monitoring in gastric cancer. Methods. Serums of 30 healthy people and 30 advanced gastric cancer patients were matched by age and gender were collected. The serum peptide spectrum was obtained by MB-WCX concentration and MALDI-TOF MS analysis. Based on the analysis of the efficiency of differential peptides in the diagnosis of gastric cancer, we first established a model for the diagnosis of gastric cancer based on differential peptides and then carried out external verification. The diagnostic reliability of this model was further tested by compared with carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9). Results. In this present study, we found the expression of two peptide peaks with a molecular weight of 2863 Da and 2953 Da were significantly increased in gastric cancer serum, while the expression of two peptide peaks with a molecular weight of 1945 Da and 2082 Da were significantly decreased. Depending on the characteristics of peptide expression, we constructed a diagnostic model, we compared the sensitivity and specificity of the model established by 2953 Da/1945 Da, and found this model is significantly higher than CEA and CA19-9. Conclusion. There were some differences in serum peptides profiles between patients with advanced gastric cancer and healthy people. The serum peptide diagnostic models based on 2953 Da and 1945 Da have high diagnostic efficiency for advanced gastric cancer. Our result indicated that this model was well worth further validation for clinical diagnosis.

Falling Trend in the Epidemiology of Gastric Cancer in Mississippi From 2003 to 2019: What Mississippi Got Right

“…Similarly, the MP3C has collaborated with community organizations and leaders to ensure an increase in the number of community health workers and patient navigators, who have been trained to ensure care access increases. Community awareness concerning resources for no-cost or low-cost cancer screening services has been promoted, with underserved neighborhoods particularly targeted in Mississippi [ 17 , 18 ]. The initiatives have been completed by collaboration with community and faith organizations, translating to culturally appropriate and comprehensive gastric cancer education.…”

“…The initiatives have been completed by collaboration with community and faith organizations, translating to culturally appropriate and comprehensive gastric cancer education. Still, the number of health and wellness initiatives has increased in Mississippi, ensuring early detection is increased by as high as 25% [ 18 ]. Across the state, therefore, the MP3C has conducted environmental scans to uncover current ministerial alliances, faith-related associations and groups, and health ministries.…”

“…Across the state, therefore, the MP3C has conducted environmental scans to uncover current ministerial alliances, faith-related associations and groups, and health ministries. In turn, resources, tools, training, and education about early gastric cancer detection have been provided, especially among the uninsured, underinsured, underserved, and minority populations [ 18 , 19 ]. The developments have been complemented by regional cancer screening referral lists that have been developed.…”

Advances in High Throughput Proteomics Profiling in Establishing Potential Biomarkers for Gastrointestinal Cancer

“…Another recent publication screened 11 differentially expressed proteins from GC plasma samples as early diagnostic biomarkers for GC [82]. Alternatively, MS-based analysis of GC cancerous and normal serum has shown that peaks at 2953 and 1945 Da have higher specificity and sensitivity than traditional CEA and CA19-9 biomarkers in GC diagnosis [83]. Another recent study proposed that DEK proteins might be used as novel biomarkers for GC [84] finding that the plasma content of DEK also reached superior sensitivity compared to the traditional biomarkers CEA, CA19.9.…”

Development and validation of a decision tree classification model for the essential hypertension based on serum protein biomarkers

“…There are several types of magnetic beads, such as the weak cation exchange (WCX) type, weak anion exchange (WAX) type, hydrophobic interaction of carbon binding group type (e.g., C3, C8, and C18), immobilized metal ion affinity chromatograph with Cu(2+) or Fe(3+) (IMAC-Cu, Fe) type, and immunoaffinity chromatography on immobilized protein G (IAC Prot G) type ( 9 ). Bioactive substances can be fixed to magnetic beads, which have large surface areas and can capture small peptides and proteins ( 10 , 11 ).…”