Study on the antioxidant activity of peptide isolated from porcine plasma during in vitro digestion

Novel Broccoli-Derived Peptides Hydrolyzed by Trypsin with Dual-Angiotensin I-Converting Enzymes and Dipeptidyl Peptidase-IV-Inhibitory Activities

“…The ultra-high-performance liquid chromatography (UPLC) coupled with a Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific) was used to identify the sequences of the peptides. All mass spectrometer parameters were followed by Zhan et al…”

Identification of a novel hypotensive peptide from porcine plasma hydrolysate by in vitro digestion and rat model

“…According to the methods of Li, Zhan, et al (2020) , the structural properties of proteins were analyzed using Fourier Transform Infrared Spectroscopy (FT/IR-4700, Jasco Corp., Tokyo, Japan). The dried plasma polypeptide powder was mixed with KBr powder in a ratio of 1:20.…”

Transepithelial transport and cellular mechanisms of food-derived antioxidant peptides

“…The peptide also up-regulated gene expression of tumor suppressor gene (Bcl-2) and HO-1 via the PI3K/Akt/mTor//Nrf2/ARE pathway, and reduced oxidant-mediated ROS production in endothelial cells, leading to the amelioration of endothelial oxidative damage and atherogenesis in high fat fed ApoE-deficient mice ( Figure 1 ). Several other FDAPs (AYI, AYL, DREI, DREL, MQIFVK, MASVPTK, EMVELPLR, VVLIGDSGVGK and LEPVIGT) were recently demonstrated to protect HepG2 cells via the Keap1/Nrf2/ARE signaling pathway, leading to the upregulation of gene expression of antioxidant enzymes and ROS detoxification pathways [ 84 , 102 , 103 ]. Similar mechanisms were demonstrated by a dipeptide (IF) from potato protein hydrolysates in hypertension-generated ROS-induced renal damage in rats [ 104 ].…”

“… Peptide/protein hydrolysate Oxidative stress model Cellular mechanism of action Ref WSREEQEREE and ADIYTEEAGR from walnut protein UV-radiation-induced mouse skin aging Suppressed NF-κB signaling pathway by preventing the activation of IκB and p-65 proteins, downregulated gene expression of interleukins (IL)-1β and IL-6, inhibited MMP-1 activity and enhanced the expression of TGF-β and procollagen type I. [ 155 ] GAGLPGKRER from Pinctada fucata protein hydrolysates UV-radiation-induced mouse skin aging Inhibited CAT, glutathione system and SOD activities, halted lipid peroxidation and suppressed UV-radiation-induced skin aging [ 86 , 130 ] PELDW, WPDHW, FGYDWW, and YLHFW isolated from Spanish Mackerel muscle H 2 O 2 -generated plasmid DNA damage Inhibited DNA damage by boosting the antioxidant status and preventing uncoiling and strand break [ 156 ] MQIFVK, MASVPTK, EMVELPLR and VVLIGDSGVGK derived from pine nut H 2 O 2 -challenged HepG2 cells and D-galactose-induced premature aging in mouse Improved the viability of HepG2 cells, and suppressed lipid peroxidation and enhanced antioxidant status by increasing SOD and GPx activity in mouse [ 84 ] LEPVIGT derived from porcine plasma H 2 O 2 -induced oxidative stress in HepG2 cells Protected HepG2 cells via Keap1-Nrf2-ARE signaling pathway [ 103 ] FYY and DW derived from lantern fish protein D-galactose-induced aging Suppressed lipid peroxidation level and expression of endothelial nitric oxide synthase, and improved memory impairment by elevating brain-derived neurotrophic factor [ 92 ] DVEDLEAGLAK and EITSLAPSTM from golden cuttlefish High fat Caenorhabditis elegans Prevented oxidative damage via upregulation of mRNA expression of DAF-16 signaling pathway-regulated genes (sod-3, catalase (cat-1) and ctl-1)) [ 54 ] AYI, AYL, DREI and DREL from Jiuzao 2,2′-Azobis(2-methylpropanimidamidine)-stressed HepG2 cells inhibited ROS generation, elevated SOD, CAT and GPx gene expression and improved the viability of HepG2 cells via Keap1-Nrf2-ARE signaling pathway [ ...…”