Viridans Group Streptococci (VGS) are the normal oral flora and etiological agents of serious infections such as infective endocarditis (IE), septicemia, meningitis and are often associated with infections in neutropenic and immunocompromised patients. The reported isolation of Staphylococcus sp from the oral cavity of healthy adults has changed the perception of looking Staphylococcus as pathogen. They could well be an oral colonizer and may associate with various diseases. The increasing resistance has shifted the treatment towards macrolides and other higher forms. In the present study an oral mouth wash was tested for its antibacterial activity against oral bacteria such as VGS and Staphylococcus sp by agar well diffusion method and minimum inhibitory concentration (MIC) assay. At 100% concentration, the oral mouth wash showed the highest zone of inhibition (25 mm) against S.mutans. MIC of the oral mouthwash against S.mutans and S.oralis was 9.37 mg/ml. To conclude our mouth wash had an excellent antibacterial activity against both the tested oral bacteria. This in turn reflects that our mouth wash could be effective against oral bacteria and can be used regularly in day to day life.