Study on the Antibreast Cancer Mechanism and Bioactive Components of Si-Wu-Tang by Cell Type‐Specific Molecular Network

Abstract

Si-Wu-Tang (SWT), a traditional Chinese herbal formula, has shown an effect on antibreast cancer. However, the mechanisms and bioactive components of SWT are still unclear. Fortunately, cell type-specific molecular network has provided an effective method. This study integrated the data of formula components, all types of biomolecules in the human body, and nonexpressed protein in breast cancer cells and constructed the breast cancer cell network and the biological network that SWT acted on the breast cancer-related targets by Entity Grammar System (EGS). Biological network showed 59 bioactive components acting on 15 breast cancer-related targets. The antibreast cancer mechanisms were summarized by enrichment analysis: regulation of cell death, response to hormone stimulation, response to organic substance, regulation of phosphorylation of amino acids, regulation of cell proliferation, regulation of signal transmission, and affection of gland development. In addition, we discovered that verbascoside played the role of antibreast cancer by inhibiting cell proliferation, but there was not a report on this effect. The results of CCK8 and western blot were consistent with the antibreast cancer effect of verbascoside based on biological network. Biological network modeling by EGS and network analysis provide an effective way for uncovering the mechanism and identifying the bioactive components of SWT.

Verbascoside—A Review of Its Antitumor Activities

“…VERB interacted with Sterile Alpha Motif Domain 3 (SAMD3), which is protein-coding gene, resulting in its phosphorylation, which in turn could hinder the cell proliferation. Additionally, VERB shown to promote the expression of activator protein 1 (AP-1), which can regulates a number of cell processes, including proliferation, differentiation and apoptosis; activated AP-1 can reduce the gene expression MYC (an oncogene) and CDk6, which is an important key in the progression of the cell cycle [54].…”

Prognostic model construction and target identification of Si-Wu-Tang against breast cancer

“…Of note, SWT has an anti-cancer effect (Shan et al, 2021) [ 11 ]. A number of previous studies have indicated that the molecular mechanism of SWT against breast cancer is closely related to its potential effects on modulation of estrogen activity [ 12 , 13 ]. By regulating ER pathway, the expression level of estrogen receptor β subtype (ER β) protein is increased, and the ratio of ER α/ER β is reduced, thus leading to the attenuation of the growth of estrogen-dependent tumor cells [ 14 , 15 ].…”

The Anti-Tumor Efficacy of Verbascoside on Ovarian Cancer via Facilitating CCN1-AKT/NF-κB Pathway-Mediated M1 Macrophage Polarization

“…VB has recently received considerable attention owing to its significant clinical value, including antioxidative, antiinflammatory, antibacterial, and antifungal properties (8)(9)(10)(11)(12). Moreover, VB also possesses anti-tumour activity against various cancers, such as glioblastoma, prostate cancer, and breast cancer (13)(14)(15)(16)(17). For example, VB has an anti-tumour role in breast cancer by suppressing cell proliferation (16).…”

“…Moreover, VB also possesses anti-tumour activity against various cancers, such as glioblastoma, prostate cancer, and breast cancer (13)(14)(15)(16)(17). For example, VB has an anti-tumour role in breast cancer by suppressing cell proliferation (16). VB can effectively inhibit cell growth and migration, and promote cell apoptosis and autophagy in glioblastoma (15).…”