Study on Sensitization from Reactive Oxygen Species for Electrochemiluminescence of Luminol in Neutral Medium

Abstract

In this paper, the sensitization on electrochemiluminescent (ECL) reaction of luminol from reactive oxygen species (ROSs) in neutral medium was studied. The powerful sensitization from ROSs even related with organics and organisms were examined under selected conditions which were suitable for biochemical analysis. The results indicated that whether the enhancers were dissolved in solutions or immobilized on the surface of conventional electrodes, stronger ECL intensity of luminol could be obtained. Enhanced ECL helped to provide groundwork for the detection of biomolecules for which would further enhance or quench the ECL signals. The technique may provide new means in a variety of fields such as clinical diagnostics, immunological analysis and environmental monitoring due to its simplicity and high efficiency.

Enhanced electrochemiluminescence from luminol at multi-walled carbon nanotubes decorated with palladium nanoparticles: A novel route for the fabrication of an oxygen sensor and a glucose biosensor

“…Superoxide and hydroxyl radicals can be generated from the reduction of oxygen during the scan of potential in negative direction and also from the oxidation and reduction of hydrogen peroxide during the scan of potential in positive and negative directions, respectively. These electrogenerated reactive species at the electrode surface can oxidize luminol radical anions, the product of the oxidation of luminol anion at the electrode surface, and consequently produce the excited state 3-aminophthalate anions which emit light [12].…”

“…In addition, nano-structure materials such as TiO 2 nanoparticles [26], multiwalled carbon nanotubes (MWCNTs) [11,14,27] and Au-NPs [13] have been also used to enhance ECL signal of luminol-H 2 O 2 system in neutral media. The sensitized luminol ECL reactions have been applied for the detection of superoxide dismutase (SOD) based on the quenching effect of SOD on luminol-O 2 ECL system by scavenging reactive oxygen species, for the detection of horseradish peroxidase (HRP) based on its enhancing effect on luminol-O 2 ECL reaction [12], and for the indirect determination of glucose [11,13,14,27] and choline [26] which react with their related enzymes and produce hydrogen peroxide. The sensitized ECL reactions make a possibility for sensitive, simple and inexpensive measurement of dissolved O 2 in the environmental, biomedical and luminol target immunological reactions [12,[28][29][30].…”

“…Luminol generates strong CL and ECL signals in alkaline media but, its ECL signals in neutral media are very weak [7]. Recently, several efforts have been devoted to sensitize and apply luminol ECL reaction for the determination of biologically important compounds in their physiological conditions [9][10][11][12][13][14][15][16].…”

The intensification of luminol electrochemiluminescence by metallic oxide nanoparticles

“…And also the previous research of authors has proven that reactive oxygen species (ROSs) could sensitize luminol's ECL [32].…”

A nano-functionalized real-time electrochemiluminescent biosensor for alanine transaminase assay

“…Due to the yielding of hydrogen peroxide, the ECL intensity of luminol would be obviously increased because hydrogen peroxide has been testified as an efficient ECL intensifier [22].…”