Study on Schiff base complexes–cellular DNA interactions by a novel system of Hadamard transform fluorescence image microscopy

Abstract

A novel system of Hadamard transform microscopic fluorescence imaging for single cells is presented, based on which the DNA ploidy of rat hepatocyte was quantitatively measured. The result shows that diploid rat hepatocyte has a stable DNA content, thus diploid rat hepatocyte was used to investigate the binding of five clinical anticancer agents, vincristine, cyclophosphamide, nitrogen mustard, cis-diamminedichloroplatinum(n) (CDDP) and mitomycin-C, with cellular DNA when acridine orange (AO) was used as the competitive fluorescence probe. Based on this model, some Schiff base complexes-cellular DNA interactions were investigated. The results indicate that all the twenty-two compounds, including Schiff base ligands of N-2-hydroxy-naphthaldehyde with D-glucoamine (NG) and the complexes of 3d-transitional metals ions with NO and with D-glucoamine (Glu) and the mixed complexes of NG and Glu series with alpha-glycine (GNG), have the ability to enter the cell membrane and interact with cellular DNA. Four of the compounds, CuGlu, Fe(II)NG, Fe(III)NG and CuGluG can intercalate with DNA like AO does and depress AO-DNA fluorescence to 70% or lower. An in intro UV-visible spectroscopic study on the compound-DNA spectra testified the above results and suggests that diverse interaction mechanisms coexist for all these complexes except intercalating mode. This study presents a new in vitro method for initial screening of anticancer compounds.

A novel water soluble ligand bridged cobalt(ii) coordination polymer of 2-oxo-1,2-dihydroquinoline-3-carbaldehyde (isonicotinic) hydrazone: evaluation of the DNA binding, protein interaction, radical scavenging and anticancer activity

“…Unlike the ligand, the Co(II) complex is highly soluble in water. The experimental μ eff value of 4.89 μ B for the water soluble complex revealed that the cobalt should be present in the complex as an octahedral high spin d 7 Co(II) species. In the X-band EPR spectra of the Co(II) complex at room temperature, a single isotropic resonance with "g" values of 2.21 and 2.17 appeared for the powdered complex and the aqueous solution of the complex, respectively, indicating that the octahedral Co(II) complex retained its solid state structure in the aqueous solution also.…”

“…A great number of Schiff base complexes of transition metal ions have triggered wide interest because of their diverse spectra of biological and pharmaceutical properties. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] In particular, transition metal complexes of typical Schiff bases, like hydrazones, semicarbazones, and thiosemicarbazones, as well as their derivative analogs, present a wide range of biological applications, such as antibacterial, antioxidant, antiproliferative, antimalarial, antitrypanosomal, anticonvulsant and antitumor, etc. [8][9][10][11][12][13][14][15][16][17][18][19] These Schiff base compounds give not only new complexes with important pharmacological properties but also exhibit interesting and varying coordination modes in their complexes.…”

Raman micro spectroscopy study of the interaction of vincristine with A549 cells supported by expression analysis of bcl-2 protein

“…A proposed primary mode of action of vincristine is through its binding to tubulin monomers in the cytoplasm, which leads to a change in the dynamics of the microtubule assembly and prevention of the formation of bi-polar spindles, 18 necessary for the proper segregation of the duplicated chromosomes and ultimately cell division. Additionally, however, vincristine has been reported to interact directly with DNA in acellular studies, as observed using techniques such as Atomic Force Microscopy, 19 Fluorescence image microscopy 20 and FTIR spectroscopy, 21 which indicate that it acts as an intercalator. The combined evidence raises the question as to whether vincristine causes cell death only by disturbing the dynamics of the microtubule assembly or whether the DNA damage caused by this anticancer agent also contributes to its efficacy.…”

“…Additionally, however, vincristine has been reported to interact directly with DNA in acellular studies, as observed using techniques such as Atomic Force Microscopy 19 , Fluorescence image microscopy 20 and FTIR spectroscopy 21 , which indicate that it acts as an intercalator. The 5 combined evidence raises the question as to whether vincristine causes cell death only by disturbing the dynamics of the microtubule assembly or whether the DNA damage caused by this anticancer agent also contributes to its efficacy.…”

DNA Interaction Studies of an Anticancer Plant Alkaloid, Vincristine, Using Fourier Transform Infrared Spectroscopy

“…Zhu et al (2004) reported about the ability of vincristine to cause structural variations in linear plasmid DNA. Tang et al (2003) indicated about its slight interaction with DNA. However, detailed information on the effect of vincristine on the structure of DNA remains unclear.…”