Study on Rhizoma Chuanxiong based on capillary electrophoresis with amperometric detection

Chu, Qingcui; Zhang, Dong Li; Zhang, Hai Tao; Ye, Jian Nong

doi:10.1016/j.cclet.2009.10.009

Cited by 7 publications

(4 citation statements)

References 5 publications

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“…CE is increasingly recognized as an important analytical separation technique because of its speed, efficiency, reproducibility, ultra-small sample volume, less consumption of solvent and ease of clearing up the contaminants [ 215 ]. CZE, which was first reported in 1981 [ 216 ], is an important model of CE.…”

Section: Analytical Methods For Simultaneous Determination Of Diffmentioning

confidence: 99%

“…In addition, with amperometric detection (AD), CE-AD affords high sensitivity and good selectivity for electroactive species. Bioactive ingredients of CX including compounds 1 , 2 , 3 , 4 , 5 , 6 , 8 , and 12 were determined by CE-AD as marker compounds, and the characteristic “electrochemical profiles” of CX was studied [ 60 , 215 ].…”

Section: Analytical Methods For Simultaneous Determination Of Diffmentioning

confidence: 99%

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Advances in the Chemical Analysis and Biological Activities of Chuanxiong

et al. 2012

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Chuanxiong Rhizoma (Chuan-Xiong, CX), the dried rhizome of Ligusticum chuanxiong Hort. (Umbelliferae), is one of the most popular plant medicines in the World. Modern research indicates that organic acids, phthalides, alkaloids, polysaccharides, ceramides and cerebrosides are main components responsible for the bioactivities and properties of CX. Because of its complex constituents, multidisciplinary techniques are needed to validate the analytical methods that support CX’s use worldwide. In the past two decades, rapid development of technology has advanced many aspects of CX research. The aim of this review is to illustrate the recent advances in the chemical analysis and biological activities of CX, and to highlight new applications and challenges. Emphasis is placed on recent trends and emerging techniques.

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Section: Analytical Methods For Simultaneous Determination Of Diffmentioning

confidence: 99%

Section: Analytical Methods For Simultaneous Determination Of Diffmentioning

confidence: 99%

Advances in the Chemical Analysis and Biological Activities of Chuanxiong

et al. 2012

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“…Rhizoma Chuanxiong, known as Chuan-xiong or ChuanChung in folk, has been used to promote blood circulation, expel wind and alleviate pain in clinical practice for thousands of years in China [1]. From Rhizoma Chuanxiong, more than 30 compounds, which belong to various different structural types, have been isolated, and among them, several components belonging to three types, namely phenolic acid, alkaloid and phthalide, have been found to be pharmacologically active [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Preparative isolation and purification of senkyunolide‐I, senkyunolide‐H and ferulic acid from Rhizoma Chuanxiong using counter‐current chromatography

Wei

et al. 2011

J of Separation Science

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Three active compounds, senkyunolide-I, senkyunolide-H and ferulic acid (FA), were successfully isolated and purified from the extracts of Rhizoma Chuanxiong by counter-current chromatography (CCC). Based on the principle of the partition coefficient values (k) for target compounds and the separation factor (α) between target compounds, the two-phase solvent system that contains n-hexane-ethyl acetate-methanol-water at an optimized volume ratio of 3:7:4:6 v/v was selected for the CCC separation, and the lower phase was employed as the mobile phase in the head-to-tail elution mode. In a single run, 400 mg of the crude extract yielded pure senkyunolide-I (6.4 mg), senkyunolide-H (1.7 mg) and FA (4.4 mg) with the purities of 98, 93 and 99%, respectively. The CCC fractions were analyzed by high-performance liquid chromatography, and the structures of the three active compounds were identified by MS and (1)H NMR.

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“…More than 90% of RC materials used in clinical are from Sichuan province. In the reported papers about fingerprint analysis of RC, the analyzed samples were mostly prepared with organic solvent, and were analyzed using HPLC with organic reagent as mobile phases, these methods were also time-consuming, usually 40-120 minutes for an analysis period [8][9][10]. The aim of this work is to develop an improved fingerprint analysis method for Rhizoma Chuanxiong and to make a better quality control for it.…”

Section: Introductionmentioning

confidence: 99%