Preparative isolation and purification of senkyunolide‐I, senkyunolide‐H and ferulic acid from Rhizoma Chuanxiong using counter‐current chromatography

Wei, Yun; Hu, Jia; Li, Hao; Liu, Jiangang

doi:10.1002/jssc.201100547

Cited by 19 publications

(6 citation statements)

References 21 publications

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“…The structure of isolated compound with melting point of 168° C is presented in Figure 1. The isolated compound was elucidated by, 1 HNMR, 13 CNMR, IR and MS as well as comparison of the data with those reported in the literature (12). The analytical data were as follows:…”

Section: Resultsmentioning

confidence: 88%

Isolation and Identification of Ferulic Acid From Aerial Parts of Kelussia odoratissima Mozaff

Sajjadi

Shokoohinia

Moayedi

2012

Jundishapur J Nat Pharm Prod

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A B S T R A C TBackground: Kelussia odoratissima Mozaff. is one of the newest genera of Umbelliferae which is represented by only one species. This sweet-smelling, self-growing monotypic medicinal plant is endemic to a restricted area in west of Iran and is locally called Karafse-koohi. The aerial parts of the plant are commonly used as a popular garnish and a sedative medicinal plant. There are several reports concerning antioxidant, antiinflammatory, anxiolytic and hypolipidemic activities of aerial parts of K. odoratissima. Objectives: The current research aimed to evaluate some phenolic contents of the plant for the first time .It is confirmed that secondary metabolites and especially phenolic compounds have important role in the biological activities of the plant. Available information indicates that phenolic contents of K. odoratissima have not been the subject of any investigation Material and Methods: Aerial parts of K. odoratissima were extracted with acetone by maceration method. Normal and reversed phase vacuum liquid chromatography used to fractionate the extract.

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Section: Resultsmentioning

confidence: 88%

Isolation and Identification of Ferulic Acid From Aerial Parts of Kelussia odoratissima Mozaff

Sajjadi

Shokoohinia

Moayedi

2012

Jundishapur J Nat Pharm Prod

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“…Besides, our previous research showed that, by comparing to raw Chuanxiong Rhizoma (RCR), the contents of butylidenephthalide, ligustilide senkyunolide A and ferulic acid were increased in wine-processed Chuanxiong Rhizoma (WCR). Additionally, these four components have many biological properties and pharmacological functions [15][16][17][18]. Therefore, they were selected as the target analytes in our experiments.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study on Pharmacokinetics of Four Active Compounds in Rat Plasma after Oral Administration of Raw and Wine Processed Chuanxiong Rhizoma

et al. 2019

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In Chinese medicine, the effect of promoting blood circulation and removing stasis could be enhanced after Chuanxiong Rhizoma is processed by wine. However, the relevant mechanism remains unclear. In this manuscript, a rapid and sensitive quantification method employing ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was established and validated to simultaneously determine butylidenephthalide, ligustilide, senkyunolide A and ferulic acid in rat plasma after oral administration of raw Chuanxiong Rhizoma (RCR) and wine-processed Chuanxiong Rhizoma (WCR) respectively. All analytes were extracted from plasma by proteins precipitation with methanol. Chromatographic separation was carried out on a Hypersil GOLD C18 column by using a gradient mobile phase system of acetonitrile and water with 0.01% formic acid, the flow rate was 0.3 mL/min. For exact mass detecting, quick switching mode was used, positive and negative ions could be detected in one injection. The pharmacokinetic profiles of four components in the two groups were evaluated and compared. The results showed that, compared to the RCR group, the Vd and AUC0→t values of four active compounds were increased and decreased respectively in WCR group, which revealed the effect of wine processing to Chuanxiong Rhizoma: the stronger the effect, the wider the distribution.

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“…Indeed, this compound can be metabolized to butylidenephthalide, senkyunolide I (M156), and senkyunolide H (M155) in vivo [15]. The three natural ingredients produce various pharmacological activities in cerebral blood vessels, the general circulatory system and immune system including spasmolysis contraction effects [16,17], inhibitory effects of platelet aggregation [18] and anti-proliferative activity, and thus improve the therapeutic effect on patients. Cnidilide (M93, OB = 77.55%) and spathulenol (M169, OB = 82.37%) also closely correlate with the smooth muscle relaxant action, and thereby have the strongest spasmolytic activity [19].…”

Section: Resultsmentioning

confidence: 99%

A Systems Biology Approach to Understanding the Mechanisms of Action of Chinese Herbs for Treatment of Cardiovascular Disease

Xue

Wang

et al. 2012

IJMS

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Traditional Chinese Medicine (TCM) involves a broad range of empirical testing and refinement and plays an important role in the health maintenance for people all over the world. However, due to the complexity of Chinese herbs, a full understanding of TCM’s action mechanisms is still unavailable despite plenty of successful applications of TCM in the treatment of various diseases, including especially cardiovascular diseases (CVD), one of the leading causes of death. Thus in the present work, by incorporating the chemical predictors, target predictors and network construction approaches, an integrated system of TCM has been constructed to systematically uncover the underlying action mechanisms of TCM. From three representative Chinese herbs, i.e., Ligusticum chuanxiong Hort., Dalbergia odorifera T. Chen and Corydalis yanhusuo WT Wang which have been widely used in CVD treatment, by combinational use of drug absorption, distribution, metabolism and excretion (ADME) screening and network pharmacology techniques, we have generated 64 bioactive ingredients and identified 54 protein targets closely associated with CVD, of which 29 are common targets (52.7%) of the three herbs. The result provides new information on the efficiency of the Chinese herbs for the treatment of CVD and also explains one of the basic theories of TCM, i.e., “multiple herbal drugs can treat one disease”. The predicted potential targets were then mapped to target-disease and target-signal pathway connections, which revealed the relationships of the active ingredients with their potential targets, diseases and signal systems. This means that for the first time, the action mechanism of these three important Chinese herbs for the treatment of CVD is uncovered, by generating and identifying both their active ingredients and novel targets specifically related to CVD, which clarifies some of the common conceptions in TCM, and thus provides clues to modernize such specific herbal medicines.

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Preparative isolation and purification of senkyunolide‐I, senkyunolide‐H and ferulic acid from Rhizoma Chuanxiong using counter‐current chromatography

Cited by 19 publications

References 21 publications

Isolation and Identification of Ferulic Acid From Aerial Parts of Kelussia odoratissima Mozaff

Isolation and Identification of Ferulic Acid From Aerial Parts of Kelussia odoratissima Mozaff

Comparative Study on Pharmacokinetics of Four Active Compounds in Rat Plasma after Oral Administration of Raw and Wine Processed Chuanxiong Rhizoma

A Systems Biology Approach to Understanding the Mechanisms of Action of Chinese Herbs for Treatment of Cardiovascular Disease

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