Study on Interaction Between TATA-Box Binding Protein (TBP), TATA-Box and Multiprotein Bridging Factor 1(MBF1) in Beauveria bassiana by Graphene-Based Electrochemical Biosensors

Abstract

The regulation of transcription level is an important step in gene expression process. Beauveria bassiana is a broad-spectrum insecticidal fungi widely used in the biologic control of arthropod. The regulation of its transcription level is a multilevel complex process. Multiprotein bridging factor 1(MBF1) is a transcriptional co-activator that bridges sequence-specific activators and the TATA-box binding protein(TBP), Little is known about the interaction between MBF1, TBP, and TBP binding to DNA(TATA-sequences)in filamentous fungi of Beauveria bassiana, The binding of TBP to TATA-box and TBP to MBF1 was investigated via electrochemical biosensor. Graphene oxide has an electronic mobility that is unattainable for any metal, so it will be highly sensitive as a test electrode. Hence, we developed a simple, sensitive and specific sensor based on an TBP probe and graphene oxide that successfully detected the interaction of TBP and TATA-box or MBF1. From the electrochemical impedance spectroscopy (EIS), we find that the radius will increase when adding TATA-box or MBF1 buffer to the modified TBP protein electrode. When adding no TATA-box or no MBF1, the radius is relatively unchanged. The interaction between TBP and TATA-box or MBF1 was proved based on the results. These data confirmed the specificity of the interactions, (1) our developed graphene-based electrochemical biosensor can be used for monitoring the interaction between TBP and TATA-box or MBF1, (2) TBP can bind to TATA-box, (3) TBP can bind to MBF1, and (4) TBP mediates the interactions of MBF1 to DNA. Therefore, this work provided a label-free, low-cost and simple detection method for the complex process of eukaryotic gene transcription regulation.

Genome-wide characterization of the MBF1 gene family and its expression pattern in different tissues and stresses in Zanthoxylum armatum

“…Multiprotein bridging factor 1 (MBF1), as one of the crucial transcriptional coactivators, is widely distributed in animals, plants, and some microorganisms, and plays a necessary role in gene expression and regulation during stress responses [ 11 , 12 ]. There are three members in the MBF1 family, including MBF1a, MBF1b, and MBF1c [ 13 ], each with a flexible N-terminal region and highly conserved helix-turn-helix (HTH) domain in its C-terminal [ 11 ].…”

Chromatin Accessibility and Transcriptional Landscape during Inhibition of Salmonella enterica by Lactobacillus reuteri in IPEC-J2 Cells

“…Further, we performed motif enrichment analysis of the altered chromatin accessibility ( Figure 5 A,B), and six common motifs were intersected among the enriched motifs ( Figure 5 C). Among these, GATA1 and TAL1 promote stress erythropoiesis and down-regulate immune response genes [ 44 ], TBP is a key protein in the transcription initiation of eukaryotic cells [ 45 ], and Gmeb1 modulates glucocorticoid receptor transactivation and inhibits cell apoptosis [ 46 ]. Some of these TFs may play a central role in the S. enterica BNCC186354 infection process and/or the antibacterial response of L. reuteri ATCC 53,608.…”

“…Integrative ATAC-seq and RNA-seq analysis provides a good analytical basis for the overall transcriptional landscape and chromatin accessibility of bacteria, and the enriched pathways indirectly represent the processes of pathogen infection and antibacterial activity. A total of eight candidate TFs, such as GATA1, TAL1, TBP, RUNX1, Gmeb1, Gfi1b, RARA, and RXRG, were identified via MEME analysis, and these TFs were associated with cell activities, such as the immune response, transcription initiation, and apoptosis [ 44 , 45 , 46 ]. Further gene annotation and enrichment analyses were used to focus on the PI3K/AKT pathway, which is one of the most important intracellular pathways, regulating cell growth, motility, survival, metabolism, and angiogenesis [ 47 , 48 ].…”