Genome-wide characterization of the MBF1 gene family and its expression pattern in different tissues and stresses in Zanthoxylum armatum

Abstract

Background Multiprotein bridging factor 1 (MBF1) is a crucial transcriptional coactivator in animals, plants, and some microorganisms, that plays a necessary role in growth development and stress tolerance. Zanthoxylum armatum is an important perennial plant for the condiments and pharmaceutical industries, whereas the potential information in the genes related to stress resistance remains poorly understood in Z. armatum. Results Herein, six representative species were selected for use in a genome-wide investigation of the MBF1 family, including Arabidopsis thaliana, Oryza sativa, Populus trichocarpa, Citrus sinensis, Ginkgo biloba, and Z. armatum. The results showed that the MBF1 genes could be divided into two groups: Group I contained the MBF1a and MBF1b subfamilies, and group II was independent of the MBF1c subfamily.. Most species have at least two different MBF1 genes, and MBF1c is usually an essential member. The three ZaMBF1 genes were respectively located on ZaChr26, ZaChr32, and ZaChr4 of Zanthoxylum chromosomes. The collinearity were occurred between three ZaMBF1 genes, and ZaMBF1c showed the collinearity between Z. armatum and both P. trichocarpa and C. sinensis. Moreover, many cis-elements associated with abiotic stress and phytohormone pathways were detected in the promoter regions of MBF1 of six representative species. The ERF binding sites were the most abundant targets in the sequences of the ZaMBF1 family, and some transcription factor sites related to floral differentiation were also identified in ZaMBF1c, such as MADS, LFY, Dof, and AP2. ZaMBF1a was observed to be very highly expressed in 25 different samples except in the seeds, and ZaMBF1c may be associated with the male and female floral initiation processes. In addition, expression in all the ZaMBF1 genes could be significantly induced by water-logging, cold stress, ethephon, methyl jasmonate, and salicylic acid treatments, especially in ZaMBF1c. Conclusion The present study carried out a comprehensive bioinformatic investigation related to the MBF1 family in six representative species, and the responsiveness of ZaMBF1 genes to various abiotic stresses and phytohormone inductions was also revealed. This work not only lays a solid foundation to uncover the biological roles of the ZaMBF1 family in Z. armatum, but also provides some broad references for conducting the MBF1 research in other plants.

Genome-Wide Analysis of MBF1 Family Genes in Five Solanaceous Plants and Functional Analysis of SlER24 in Salt Stress

“…A total of 160 amino acid sequences from 43 species ( Table S4 ) were used to construct a phylogenetic evolutionary tree, which illustrates the evolutionary relationships of the MBF1 family ( Figure 2 ). Consistent with previous research findings [ 1 , 2 ], the MBF1 family can be divided into two groups: group I consists of the MBF1a and MBF1b subfamilies, and group II includes only the MBF1c subfamily. It is noteworthy that MBF1a and MBF1b have a closer relationship, suggesting that they may have similar functions.…”

“…A total of 160 amino acid sequences from 43 species (Table S4) were used to construct a phylogenetic evolutionary tree, which illustrates the evolutionary relationships of the MBF1 family (Figure 2). Consistent with previous research findings [1,2], the MBF1 family can be divided into two groups: group I consists of the MBF1a and MBF1b subfamilies,…”

“…MBF1 is a crucial transcriptional coactivator found in animals, plants, and microorganisms, playing a vital role in growth, development, and stress tolerance [ 1 , 2 , 36 , 37 , 38 , 39 ]. Nevertheless, there is a scarcity of comprehensive information regarding the genome-wide identification and characterization of the MBF1 gene family in Solanaceous species.…”

“…It plays a critical role in the regulation of gene expression during stress responses [ 1 ]. MBF1 consists of two domains: the N-terminal multiprotein bridging factor 1 (MBF1) domain and the C-terminal helix-turn-helix (HTH) domain [ 1 , 2 ]. The HTH domain is essential for maintaining the functional activity of MBF1 [ 3 ].…”

Multi-omics research on common allergens during the ripening of pollen and poplar flocs of Populus deltoides

“…In addition, MBF1 and cysteine proteinase inhibitor were most significantly up-regulated during pollen maturation. MBF1 is an important transcriptional coactivator, which is expressed differently in plant tissues and is essential for gene expression and regulation in abiotic stress response (Hui et al, 2022). Overexpression of MBF1 in arabidopsis reduces its tolerance to low temperature and high salinity stress (Guo et al, 2014).…”