Study on In-vivo anti-tumor activity of <i>Verbena officinalis</i> extract

Abstract

We investigated the anti-tumor effects of Verbena officinalis extract on H22 tumor-bearing mice and its effect on immune function. Mice model of H22 solid tumor was established, the mice were divided into five groups and administered the extract, later, tumors were removed and inhibition rates were calculated; spleens were removed and spleen indices were calculated, and the sheep red blood cell-delayed-type hypersensitivity (SRBC-DTH) and the serum hemolysin level were determined. The Verbena officinalis extract had anti-tumor effect, with the inhibition rate reaching 38.78%, it also increased the spleen index to a certain extent, in addition, the changes in DTA and HA were not obvious compared with the model group. The Verbena officinalis extract had in vivo anti-tumor effect, while causing no damage on the immune function.

Assessment of antioxidant and antibacterial activities of crude extracts of verbena officinalis Linn root or Atuch (Amharic)

“…It is useful when taken internally in the treatment of ailments such as headaches, fevers, nervous exhaustion, depression, and gallbladder problems and insufficient lactation (Kou et al, 2013). Externally, it is used to treat minor injuries, sores neuralgia and gum disease.…”

“…The quantity and quality of phytochemicals present in different parts of a plant may vary from one part to another or from plant to another plant (Kou et al, 2013). In this study, the active components of root extracts of V.officinalis, one of the most widely used medicinal plants, were qualitatively analyzed.…”

Anticonvulsant, Anxiolytic, and Sedative Activities of Verbena officinalis

“…It grows up to height of about 1 m, having lobed and toothed leaves, while flowers are elegant, silky and pale purple in color (Voogelbreinde, 2009). In traditional system of medicine, Verbena officinalis has been used for treatment of melancholia, hysteria, seizures, jaundice, fever, cholecystaliga, anxiety, depression, insomnia, menstrual disorders (Khare, 2007), abdominal problems, malaria, pharyngitis, edema (Kou et al, 2013), cough, asthma (Vitalini et al, 2009), rheumatic and thyroid problems (Guarrera et al, 2005) etc. The constituents isolated from Verbena officinalis include verbenin, verbenalin, hastatoside, alpha-sitosterol, ursolic acid, oleanolic acid (Duke, 1992), kaempferol, luteolin (Chen et al, 2006), verbascoside, aucubin, apigenin, scutellarein (Rehecho et al, 2011) and essential oils like limonene, cineole, spathulenol, ar-curcumeme (Chalchat and Garry, 1995).…”

“…The constituents isolated from Verbena officinalis include verbenin, verbenalin, hastatoside, alpha-sitosterol, ursolic acid, oleanolic acid (Duke, 1992), kaempferol, luteolin (Chen et al, 2006), verbascoside, aucubin, apigenin, scutellarein (Rehecho et al, 2011) and essential oils like limonene, cineole, spathulenol, ar-curcumeme (Chalchat and Garry, 1995). The plant is reported to possess antitussive (Gui and Tang, 1985), analgesic, anti-inflammatory (Calvo, 2006), neuroprotective (Lai et al, 2006), Antiradical (Speroni et al, 2007), antioxidant, antifungal (López et al, 2008), anti-tumor (Kou et al, 2013), antibacterial (Mengiste et al, 2015), Antiproliferative (Encalada et al, 2015), and antidepressant (Kamal et al, 2015) activities. In this investigation, we report anticonvulsant, anxiolytic and sedative actions of Verbena officinalis , which explains its ethno-medicinal use in neurological disorders.…”

Antiproliferative effect of phenylethanoid glycosides from Verbena officinalis L. on Colon Cancer Cell Lines

“…In the last years, several studies have also shown antitumor activity of this plant against human choriocarcinoma JAR cells (Zhang, Luo, Wang, & Xu, 2004), chronic lymphocytic leukaemia cells (De Martino et al, 2009), and solid tumor (Kou et al, 2013).…”