Antiproliferative effect of phenylethanoid glycosides from Verbena officinalis L. on Colon Cancer Cell Lines

Anticonvulsant, Anxiolytic, and Sedative Activities of Verbena officinalis

“…The constituents isolated from Verbena officinalis include verbenin, verbenalin, hastatoside, alpha-sitosterol, ursolic acid, oleanolic acid (Duke, 1992), kaempferol, luteolin (Chen et al, 2006), verbascoside, aucubin, apigenin, scutellarein (Rehecho et al, 2011) and essential oils like limonene, cineole, spathulenol, ar-curcumeme (Chalchat and Garry, 1995). The plant is reported to possess antitussive (Gui and Tang, 1985), analgesic, anti-inflammatory (Calvo, 2006), neuroprotective (Lai et al, 2006), Antiradical (Speroni et al, 2007), antioxidant, antifungal (López et al, 2008), anti-tumor (Kou et al, 2013), antibacterial (Mengiste et al, 2015), Antiproliferative (Encalada et al, 2015), and antidepressant (Kamal et al, 2015) activities. In this investigation, we report anticonvulsant, anxiolytic and sedative actions of Verbena officinalis , which explains its ethno-medicinal use in neurological disorders.…”

Verbena officinalis (Common Vervain) – A Review on the Investigations of This Medicinally Important Plant Species

“…The biological activities of V. officinalis have been studied in vitro on cell lines. These studies deals with the neuroprotective effect on rat cortical neurons culture [40], selective apoptosis induction of neoplastic cells in leukocytes isolated from human blood [70], proapoptotic activity on lymphocytes from patients with untreated chronic lymphocytic leukemia [42], and antiproliferative activity on the rat colonic epithelial cell line DHD/K12/ PROb and human colon adenocarcinoma cell line HCT-116 [69] (▶ Table 3). All mentioned studies concern mechanisms important in the treatment of civilization diseases (Alzheimerʼs disease and cancer treatment) but they are still a long way from in vitro experiments to use in human treatment.…”

“…Iridoids verbenalin and its derivative [31,50] 3,4-dihydroverbenalin [31] hastatoside and its derivative [31,84] 7-hydroxydehydrohastatoside [31] aucubin [38,49] verbeofflin [31] verbenoside A [85] verbenoside B [85] Phenylpropanoid glycosides verbascoside and its derivatives: 6-acetyl-O-verbascoside, 4-acetyl-O-verbascoside, 2,4-diacetyl-O-verbascoside [50,69] β-hydroxy verbascoside [84] isoverbascoside and its derivatives [37,69] 4-acetyl-O-isoverbascoside [69] 3,4-diacetyl-O-isoverbascoside [69] β-hydroxy isoverbascoside [84] eukovoside [84] campenoside II [69] isocampenoside II [69] betonyoside A and its derivatives:…”

“…A weaker, but also very strong, activity was shown by 6′′-acetyl-Overbascoside, 4′′′-acetyl-O-verbascoside, and 4′′′O-acetyl-O-isoverbascoside. Research has shown that diacetylphenylpropanoids can be valuable chemopreventive compounds [69].…”

Application of LC-DAD Metabolic Fingerprinting in Combination with PCA for Evaluation of Seasonality and Extraction Method on the Chemical Composition of Accessions from Lippia alba (Mill) N. E. Brown and Biological Activities

“…Consequently, the chromatographic analysis of such preparations is not trivial, therefore, it is necessary the development of a separation method specific for each type of sample. 21,33 Thus, in order to obtain the fingerprint chromatograms of L. alba extracts, previous optimizations were carried out by evaluating the following factors: the nature of the stationary phase, such as Kinetex C18 (250 × 4.6 mm, 5 µm) and Kinetex phenyl-hexyl (150 × 4.6 mm, 5 µm; Phenomenex); organic modifier (methanol and acetonitrile); the effect of whether using or not an acidic aqueous solution (with 0.5% v/v formic acid); column temperature (24,30,35,40,45 and 50 o C); mobile phase flow (0.8, 1.0 and 1.2 mL min -1 ); gradient shape and wavelength for the detection of compounds (between 200 and 400 nm, based on the secondary metabolites commonly found in this species). [34][35][36] The best separation method was obtained by using these analyses conditions: Kinetex C18 column; mobile phase consisting of 0.5% aqueous formic acid (v/v; A) and acetonitrile (B); solvent flow rate: 0.8 mL min -1 , temperature of 45 o C and wavelength of 240 nm, which was selected from the 3D graph obtained by the use of diode array detector, using gradient elution.…”