Application of LC-DAD Metabolic Fingerprinting in Combination with PCA for Evaluation of Seasonality and Extraction Method on the Chemical Composition of Accessions from Lippia alba (Mill) N. E. Brown and Biological Activities

Abstract

The methodology developed in this study through the acquisition of fingerprint chromatograms by liquid chromatography-diode array detector (LC-DAD), aided by principal component analysis, made it possible to assess, in a rational way, the chemical differences between six accessions of Lippia alba and to verify the influence of the method of extraction, the seasonality and the individuality of each accession on chemical composition of its extracts. Among all extracts analyzed against cancer cell lines, eight of them showed to be more promising against a human leukemia cell line (HL-60), displaying cell growth inhibition percentage ranging between 40.0 and 52.0%. In the inhibitory activity assays against acetylcholinesterase enzyme, all extracts showed weak inhibitory effect, with highlight only for six of them, which displayed inhibition ranging between 27.0 and 32.0%.

Metabolomic study of Virola surinamensis (Rol.) Warb raw seed butter from different Amazonian regions

“…In addition, statistical analysis of the complex data obtained is also a powerful tool to the metabolomics field. 10,11 The objective of this work was to study the metabolomic differences of ucuuba (Virola surinamensis) raw seed butter from three distinct Amazonian regions in order to characterize the chemical differences between samples of the same species provided from different environments. Advanced analytical techniques (GC-FID and UPLC-ESI-QToF-MS) were used to provide information about primary and secondary metabolites present in the samples.…”