Study on immune function of dendritic cells in patients with esophageal carcinoma

Abstract

AIM:To investigate the immune function of dendritic cells from both peripheral blood and operated tissues of esophageal carcinoma patients in order to find the relationship between the immune function of dendritic cells and the pathogenesis of esophageal carcinoma. METHODS:The expression of CD83, CD80, and CD86 on the surface of dendritic cells cultured from the peripheral blood of patients was detected compared with that from health donors using flow cytometry. The ability of dendritic cells to induce T lymphocyte proliferation was evaluated by a liquid scintillation counter. The expression of CD80, CD86, CD83, and S-100 proteins was assessed in esophageal carcinoma tissues using immunohistochemical method. RESULTS:Compared with those from healthy donors, dendirtic cells cultured from the peripheral blood of patients expressed lower CD80 and CD86. Furthermore, the ability of dendritic cells in patients to induce T lymphocyte proliferation was significantly lower than that of the control group. Compared with the control group, the positive expression ratio and frequencies of CD80, CD86, and S-100 in esophageal carcinoma tissues were significantly down regulated. The expression of CD83 was up-regulated in the pericancerous tissues, but no expression was found in the cancerous nodules. CONCLUSION:The impaired immune function and the decreased number of dendritic cells cause pathogenesis and progression of esophageal carcinoma.

Expression and distribution of S-100, CD83 and apoptosis-related proteins (Fas, FasL and Bcl-2) in tissues of thyroid carcinoma

“…Due to a lack of a human DC-specific marker, S100 protein-positive cells, interdigitating cells (IDC) and HLA-DR positive cells have been studied as functional DCs in different pathological conditions (Demetris et al 1998;Ishigami et al 1998;Gabrilovich et al 1997;Steinman 1991;Hart 1997). But, recent advances in DC research indicate that IDC probably represent a population of DCs undergoing apoptosis and DCs expressing HLA-DR without co-stimulation would only induce T cells energy, but not T cell stimulation, whereas, there is no known function of S100-positive cells (Hart et al 1997;Chen et al 2004). In addition, T cell activation requires engagement of co-stimulatory receptors on the T cells.…”

“…The importance of DC83 and the CD80/CD86-CD28 system in T cell responses has been well demonstrated (Avigan. 1999;Chen et al 2004;Mogi et al 2000). The CD80 and CD86, members of the immunoglobulin super gene family, are encoded by separate genes and provide co-stimulatory function for APCdependent T-cell activation both in vivo and in vitro (Zai et al 2001;Zhang et al 1998;Ito et al 2001 ;Xing et al 2000).…”

Immunologic Function of Dendritic Cells in Esophageal Cancer

“…Chen [44] investigated the immune function of dendritic cells from both peripheral blood and operated tissues of esophageal cancer patients in order to find the relationship between the immune function of dendritic cells and the pathogenesis of esophageal cancer. The expression of CD83, CD80, and CD86 on the surface of dendritic cells cultured from the peripheral blood of patients was detected compared with that from healthy donors using flow cytometry.…”

“…DCs can ingest apoptotic tumor cells (ATC) and present tumor-associated antigens (TAA) to T cells, leading to the generation of tumor-specific cytotoxic effect cells [6]. However, the immune functions of DCs are often suppressed under some tumor-bearing states [7].…”

Análise do fenômeno da imunotolerância na fotocarcinogênese em lábio

“…Tais células CD83 + possuem uma capacidade apresentadora de antígeno muito poderosa90 . Em casos de carcinoma esofágico, por exemplo, a expressão dessas células está diminuída91 . Da mesma forma, observou-se ausência ou um recrutamento mínimos de células dendríticas maduras em câncer de próstata 92 , carcinoma hepatocelular 93 e melanoma metastático94 .Neste trabalho, os dados encontrados estão de acordo com esse último relato, pois a presença de células dendríticas maduras (CD83 + ) foi mais expressiva nos casos de CEL comparativamente.…”