Study on Extraction of Mucopolysaccharide-protein Containing Chondroitin Sulfate from Chicken Keel Cartilage Electrophoresis

Abstract

The objective of this study was to investigate technical methods for extraction of mucopolysachharide-protein containing chondroitin sulfate from keel cartilage of chickens. The chemical composition of chicken keel cartilage was determined. For the preparation of mucopolysaccharide-protein from lyophilized chicken keel cartilage, hot water extraction and alcalase hydrolysis methods were examined. Results showed that the optimum condition of hot water extraction was incubation for 120 min with a yield of 40.09% and chondroitin sulfate content of 28.46%. For alcalase hydrolysis, the most effective condition was 2% alcalase in 10 volumes of distilled water for 120 min. The yield of hydrolysate was 75.87%, and chondroitin sulfate content was 26.61%. For further separation of chondroitin sulfate from the alcalase hydrolysate, which has a higher yield than that of hot water, 60% ethanol precipitation was performed. The yield of the ethanol precipitate was 21.41% and its chondroitin sulfate content was 46.31%. The hot water extract, alcalase hydrolysate and ethanol precipitate showed similar electrophoretic migration with standard chondroitin sulfate (chondroitin sulfate A), using cellulose acetate membrane electrophoresis. These results indicated that a significant amount of mucopolysaccharideprotein containing chondroitin sulfate could be acquired form chicken keel cartilage. Therefore, keel cartilage in chicken may provide an inexpensive source of chondroitin sulfate for commercial purposes.

HCTN 60-Extraction of Chondroitin Sulfate From Chicken Keel Cartilage by Combining of Ultrasound Treatment and Alcalase Hydrolysis

“…The chemical compositions of chicken keel cartilages were determined and shown in Table 1. The content of the basic components similar to the analysis results of Shin et al [4].…”

“…The result in Table 4 showed that moisture content 7.4 % is similar to the result of the study of S.C. Shin [4], protein content 6.05 % is lower, the content of carbohydrate is similar. By HPLC method, researched CS obtained the purity of 80.45 % ( Figure 5) with the average molecular weight ( w ) of 159.53kDa by GPC (Table 5 and Figure 6).…”

Chondroitin sulfate extraction from broiler chicken cartilage by tissue autolysis

“…Keel cartilage CS-peptide has been extracted by several researchers (Garnjanagoonchorn et al, 2007;Luo et al, 2002;Shin et al, 2006). However, these authors have not provided basic analytical data (e.g.…”

“…Nakano et al (1998) incubated bovine nasal cartilage in 0.1 mol/L sodium acetate at pH 4.5 and 37 C and reported that approximately 80 g/100 g of total uronic acid was released from the tissue. Nakano et al (2000) then reported extraction of more than 70 g/100 g of total uronic acid by incubating bovine nasal cartilage in acidified water at pH 4.5 and 37 C. Shen and Ghosh (2004) incubated bovine tracheal cartilage in 0.1 mol/L calcium acetate, pH 4.5 at 37 C and reported that the GAG-peptide extracted is substantially free of contaminating DNA. There are, however, few reports of extraction of GAG from chicken tissues by using the autolysis technique.…”

“…However, proteolysis with exogenous enzyme(s) is a more common method to extract CS together with other GAGs from tissues. GAGs including CS have been extracted from chicken cartilage by proteolysis with papain (Garnjanagoonchorn, Wongekalak, & Engkagul, 2007) and alcalase (Shin, You, An & Kang, 2006). CS has also been extracted from bovine cartilage by activation of endogenous proteinases (autolysis) without using exogenous enzyme (Nakano, Ikawa, & Ozimek, 2000;Nakano, Nakano, & Sim, 1998).…”