To fulfill the increasing demand for dyes and pigments by the textile industries syntactic dyes comes into existence, which caused a serious environmental problem. Natural dyes are one such alternative to this problem. In this regard the present study was conducted to optimize extraction protocol (by using Box-Behnken Design) and chemical characterization of dye from the Lantana camara. The optimized point for extraction of flower and leaf dye was achieved at 92 0 C, 174 minutes with Mass to liquor ratio MLR 2:100, yielded 29.70% dye and 79 0 C, 36 minutes with MLR 3:100 yielded 26.2% dye respectively. The Methanol and Ethanol extract of flower and leaf dye was subjected to phytochemical screening by using GC-MS and dye extract showed that the presence of n-Hexadecanoic acid and 1,2-Benzenedicarboxylic acid, bis(2-ethylhexyl) in higher concentration. Most of the chemicals found in the dye extract have antibacterial, antifungal and Anti-Inflammatory properties. Hence, the use of the Lantana camara leaf and flower will reduce the pollution and helps in the management of this invasive weed in the Indian forests.