Study on Chemical Constituents of the Lichen Parmotrema Tinctorum (Nyl.) Hale (Parmeliaceae)

Abstract

A phytochemical investigation was conducted on a foliose lichen, Parmotrema tinctorum (Nyl.) Hale, collected in Lam Dong province, Vietnam. Colour reactions for identification of lichen substances (+K deep yellow, +C red, +KC red, + P pale yellow) suggested the presence of atranorin, lecanoric acid, quinones, depsides, and xanthones containing two free hydroxyl groups in meta-position. Chemical constituent study led to the isolation of six compounds, including atranol (1), methyl haematomate (2), divaricatinic acid (3), methyl divaricatinate (4), atranorin (5) and lecanoric acid (6). Their structures were confirmed unambiguously by X-ray diffraction, spectroscopic data and compared with those in references.

UHPLC-MS Metabolomic Fingerprinting, Antioxidant, and Enzyme Inhibition Activities of Himantormia lugubris from Antarctica

“…Usnic acid: (300 MHz, CDCl 3 ): 1.76 (3H, s, Me-13), 2.11 (3H, s, Me-16), 2.66 (3H, s, Me-15), 2.77 (3H, s, Me-18), 5.95 (lH, s, H-4), 11.30 (lH, s, HO-10), 14.36 (lH, s, HO-8), 18.70 (lH, s, HO-3) [55]; barbatolic acid: 1 H-NMR (300 MHz, CDCl 3 -DMSO-d6): 2.44 (3H, s, Me-9), 5.28 (2H, s, -CH 2 -9'), 6.36 (lH, s, H-5), 6.48 (2H, s, H-l', H-5'), 10.34 (lH, s-CHO-8); 5,7-dihydroxy-6-methylphthalide: 1 H-NMR (300 MHz, CDCl 3 ): 2.22 (3H, s, Me), 6.45 (lH, s, arom.-H), 5.35 (CH 2 -), 9.50 (lH, s, -OH), 12.06 (lH, s, -OH) [56]; atranol: 1 H-NMR (300 MHz, CDCl 3 ): 2.31 (3H, s, Me), 6.32 (lH, s, arom. -H), 6.40 (lH, s, -OH), 10.20 (lH, s, CHO), 11.08 (lH, s, -OH) [57]. The purity of the isolated compounds (>95%) was determined based on HPLC-PDA analysis.…”