Chemical Constituents of the Lichen Parmotrema Tinctorum and their Antifungal Activity

Tuân, Nguyễn Trọng; Dam, Nguyen Phuc; Van, Hieu; Trang, Đái Thị Xuân; Danh, Luu Thai; Men, Tran Thanh; De, Tran Quang; Bạch, Lê Thị; Kanaori, Kenji

doi:10.1007/s10600-020-03017-y

Cited by 13 publications

(3 citation statements)

References 14 publications

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“…Lichen acids, atranorin, and lecanoric acid were detected in the TLC profile of P. tinctorum and the GC-MS analysis confirmed the presence of atraric acid, orcinol, and O-orsellinaldehyde among the major constituents. Previous studies have also detected O-orsellinaldehyde and orcinol as bioactive derivatives obtained from lecanoric acid [38][39][40]. Similar antimicrobial activities were reported by Gomes et al for the lecanoric acid isolated from the lichen, P. tinctorum [41].…”

Section: Discussionsupporting

confidence: 81%

Chemical Composition and Antimicrobial Activity of Two Sri Lankan Lichens, Parmotrema rampoddense, and Parmotrema tinctorum against Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus

Shiromi

Hewawasam

Jayalal

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Introduction. Medicinal utility of lichens is ascribed to the presence of various secondary metabolites of low molecular weight and they have been used in traditional medicine including Ayurveda in the treatment of wounds and skin disorders. Despite the urgent need to effectively address the antibiotic resistance worldwide, the discovery of new antibacterial drugs has declined in the recent past. This emphasizes the increasing importance of investigating and developing new classes of antibiotics that can withstand antibiotic resistance. Aims of the study. The present study was conducted to investigate the chemical composition and the antibacterial activity of hexane, ethanol, and aqueous extracts of Parmotrema rampoddense and Parmotrema tinctorum, two lichens collected from Belihuloya, Sri Lanka, against Gram-negative and Gram-positive bacteria including twenty clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). Materials and methods. Phytochemical analysis, thin layer chromatography (TLC), and Gas Chromatography Mass Spectrometry (GC-MS) were performed to determine the chemical composition of the two lichens. Hexane, ethanol, and aqueous extracts of both lichens were tested against clinical isolate of Gram-negative and Gram-positive bacteria including twenty clinical isolates of MRSA. Bacterial susceptibility was tested using a disc diffusion assay. Minimum inhibitory concentration (MIC) was determined by a broth microdilution method. Vancomycin was used as the positive control. Results. Alectorialic acid, atranorin, atraric acid, orcinol, and O-orsellinaldehyde were among the secondary metabolites identified by the TLC and GC-MS analysis. None of the lichen extracts were active against Gram-negative bacteria but both lichens showed a concentration-dependent activity against methicillin-sensitive Staphylococcus aureus (MSSA) and MRSA. Ethanol extract of P. rampoddense showed the highest activity against MSSA with the MIC, 0.0192 mg/ml, but all MRSA isolates investigated showed MIC between 0.096 and 2.4 mg/ml for the same extract. Conclusion. Both lichens, P. rampoddense and P. tinctorum, represent potentially important sources of future antimicrobial drugs. Further investigation on the ethanol extract of P. rampoddense will enable us to determine the most active phytoconstituents responsible for the activity, their mechanism of action against bacterial pathogens, and also their cytotoxicity against normal cells.

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Section: Discussionsupporting

confidence: 81%

Chemical Composition and Antimicrobial Activity of Two Sri Lankan Lichens, Parmotrema rampoddense, and Parmotrema tinctorum against Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus

Shiromi

Hewawasam

Jayalal

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…Dentre os liquens que demonstraram potencial antimicrobiano contra as bactérias patogênicas selecionadas para este projeto, LC12 e LC17, identificados como pertencentes ao gênero Parmotrema, o qual é amplamente estudado no que tange em especial as propriedade de seus extratos e compostos/moléculas acerca de propiedades antimicrobianas, antioxidantes, antifúngicas, combate a patógenos de plantas e até estudos associados à poluição atmosférica sobre sua fisiologia (Pradhan et al, 2022;Tuan et al, 2020;Bogo et al, 2020;Tartouga et al, 2022;Saha;Pal;Paul;Santos et al, 2022). O gênero também é um grande portador de fungos endoliquênicos (Chakarwarti;Nayaka;srivastava, 2020).…”

Section: Discussionunclassified

Análise do potencial antimicrobiano de liquens em culturas de microrganismos patogênicas

Arêdes Diniz,

Azevedo Lima Barbastefano

2024

PIC

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Um dos maiores desafios no que tange a saúde pública em aspecto global é a criseantibiótica, pois existe uma vasta literatura científica acerca de tal problemática ondeautoridades no assunto afirmam ser uma questão de urgência, caso contrário o cenáriofuturo não é otimista e promissor para a humanidade neste sentido. Ciente deste obstáculo,pesquisadores de diversas áreas investir e pesquisar nesta problemática, onde muitostrabalhos realizados demonstram a existência de organismos que possuem algumapropriedade antimicrobiana contra patógenos de interesse médico, e dentre tais organismos,os liquens são um grupo alvo de estudos atrelados a tal questão. Já existem pesquisasdemonstrando a propriedade antimicrobiana que esses seres apresentam.. Sendo assim, opresente trabalho buscou investigar o potencial antimicrobiano de extratos de liquensoriundos do Parque da Cidade Dona Sarah Kubitschek contra três bactérias patogênicas,Escherichia coli, Staphylococcus aureus e Enterococcus faecalis. Foram desenhadas duasmetodologias para a execução dos experimentos (nomeadas como M1 e M2), as duasorbitam ao redor de um ponto central: a aplicação dos extratos contra colônias das bactériassupracitadas sobre discos de difusão. A semeadura dos inóculos bacterianos foi feita em emplacas de petri com meio de cultura Mueller-Hinton. Foi possível observar a formação dehalos, indicando sensibilidade das bactérias contra o extrato. Das 17 amostras coletadas, trêsapresentaram resultados interessantes, sendo elas as amostras LC11, LC12 e LC17, contraS.aureus, E.coli e E.faecalis respectivamente. Interessante destacar que todas as amostrascoletadas possuíam uma morfologia foliosa. Das três amostras de interesse, LC11 foisubmetida à metodologia M1 e LC12 e LC17 à M2, dentre elas, o halo mais marcante enotório foi o do extrato obtido de LC11 contra S.aureus. A partir dos resultados obtidos aolongo deste trabalho, o Parque da Cidade Dona Sarah Kubitschek pode ser considerado umlocal portador de uma comunidade liquênica de interesse para futuros estudos com enfoquena problemática envolvendo a crise antibiótica.

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“…(Roccellaceae) is a genus of fruticose lichens that comprises more than 20 species, 7 of which are widespread in Europe, especially along the Mediterranean coasts [ 4 ]. Under the phytochemical point of view, about fifty secondary metabolites belonging to several classes of compounds have been reported for the Roccella genus, including depsides (e.g., erythrin, lecanoric acid, and lepraric acid), monoaromatic phenols (e.g., β-orcinol, ethyl orsellinate, and montagnetol), and aliphatic acids (e.g., roccellic acid) [ 5 ], which have already been reported to have strong effects against different fungal strains [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Antifungal and Antiproliferative Properties of the Lichen Roccella tinctoria DC. Extracts and Main Components

Frezza,

Fraioli,

Conti

et al. 2024

Pharmaceutics

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In this work, phytochemical analysis on different extracts of Roccella tinctoria DC. was reported using different techniques with respect to the past. Twenty volatile and three non-volatile compounds were identified, some of which were found in this species for the first time. The methanolic extracts and their non-volatile components were then evaluated for their antitumor effects in cancerous A549 and Mz-ChA-1 cells and for their tolerability in non-cancerous BEAS-2B and H69 cells, showing IC50 values from 94.6 µg/mL to 416.4 µg/mL, in general. The same extracts and compounds were also tested for their antifungal effects in Candida albicans, with only compound 2 being active, with an MIC50 value of 87 µg/mL. In addition, they were tested for their anti-Candida adhesion activity, anti-Candida biofilm formation, and anti-Candida mature biofilm inhibition, with efficacy percentages generally above 50% but not for all of them. Lastly, the DF3 extract and compounds 1–2 were tested in vivo according to the Galleria mellonella survival assay, showing positive mortality rates above 50% at different concentrations. All these biological assays were conducted on this species for the first time. Comparisons with other lichens and compounds were also presented and discussed.

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Chemical Constituents of the Lichen Parmotrema Tinctorum and their Antifungal Activity

Cited by 13 publications

References 14 publications

Chemical Composition and Antimicrobial Activity of Two Sri Lankan Lichens, Parmotrema rampoddense, and Parmotrema tinctorum against Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus

Chemical Composition and Antimicrobial Activity of Two Sri Lankan Lichens, Parmotrema rampoddense, and Parmotrema tinctorum against Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus

Análise do potencial antimicrobiano de liquens em culturas de microrganismos patogênicas

Evaluation of the Antifungal and Antiproliferative Properties of the Lichen Roccella tinctoria DC. Extracts and Main Components

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