Study on Chemical Constituents in Polygoni Cuspidati Folium and its Preparation by UPLC-ESI-Q-TOF-MS/MS

Wang, Xin; Qin, Yao; Li, Guang-quan; Chen, Shuai; Jing-qi, MA; Guo, Yanlei; Luo, Wenjing

doi:10.1093/chromsci/bmy017

Cited by 18 publications

(11 citation statements)

References 13 publications

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“…The precursor ion of compound 39 is m/z 193.0506 [M − H] − , and fragment ions are m/z 178.06 [M − CH 3 ] − , m/z 149.0611 [M − H − CO 2 ] − , and m/z 134.0375 [M − H − CO 2 − CH 3 ] − were obtained, which is consistent with the fragmentation pattern of ferulic acid reported in the literature, 32 so it is determined to be ferulic acid.…”

Section: Resultssupporting

confidence: 89%

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UPLC–MS/MS method for the determination of the herb composition of Tangshen formula and the in vivo pharmacokinetics of its metabolites in rat plasma

Yu²,

Cai

et al. 2021

Phytochemical Analysis

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Introduction Tangshen formula (TSF) is a traditional Chinese medicine composed of seven medicinal herbs including Astragalus membranaceus, Rehmannia glutinosa Libosch, Citrus aurantium L., etc. which is used to treat diabetic nephropathy III, IV qi and yin deficiency and stasis syndrome. Most of the studies on TSF are pharmacological and pharmacodynamic experiments. There are few basic studies on its chemical substances, and the effective constituents are not clear. Objective To analyse the main chemical components of TSF and the absorbed components in rat plasma following oral administration based on liquid chromatography tandem mass spectrometry (LC–MS/MS). Moreover, providing a rapid and valid analytical strategy for simultaneous determination of six components in rat plasma and use it in pharmacokinetic studies. Results A total of 132 components were identified in TSF, and 44 components were identified in rat plasma after oral TSF, 35 of which were prototype components and nine were metabolic components. A sensitive and reliable LC–MS/MS method was developed for simultaneous determination of six components in rat plasma. The intra‐day and inter‐day precision relative standard deviation (RSD) was lower than 15%; the accuracy of low, medium and high concentrations ranged from 80% to 120%. The recovery met the requirements and the RSD of the recoveries was less than 15%. Conclusion A total of 132 components were identified in TSF. The LC–MS/MS quantitative method for the simultaneous determination of morroniside, loganin, notoginsenoside R1, ginsenoside Re, ginsenoside Rb1 and astragaloside IV in rat plasma was established for the first time. The pharmacokinetic parameters are clarified, which can guide the clinical medication of TSF.

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Section: Resultssupporting

confidence: 89%

“…, which is consistent with the fragmentation pattern of citric acid reported in the literature, 14 so it is determined to be citric acid. obtained, which is consistent with the fragmentation pattern of ferulic acid reported in the literature, 32 so it is determined to be ferulic acid.…”

Section: Fragmentation Of Organic Acidssupporting

confidence: 90%

UPLC–MS/MS method for the determination of the herb composition of Tangshen formula and the in vivo pharmacokinetics of its metabolites in rat plasma

Yu²,

Cai

et al. 2021

Phytochemical Analysis

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“…Both of the deprotonated molecular ions yield [M − H + CH 3 COOH − H 2 O] − at m / z 503 and m / z 665. Compound 39 produced their [M − H + CH 3 COOH − 2H 2 O] − , [M − H − CH 2 ] − , [M − H − Glc − CO] − , [M − H − Glc − C 5 H 10 O 5 ] − and [M − H − Glc − C 5 H 10 O 5 − CH 2 ] − ions at m / z 647, m / z 609, m / z 433, m / z 311 and m / z 297 [52]. Compound 46 produced their [M − H + CH 3 COOH − CO 2 ] − , [M − H − C 4 H 8 O 4 ] − , [M − H − C 5 H 10 O 5 − CH 2 ] − and [M − H − C 5 H 10 O 5 − C 9 H 8 O 2 ] − ions at m / z 477, m / z 341, m / z 297 and m / z 163.…”

Section: Resultsunclassified

Rapid Characterization and Identification of Chemical Constituents in Gentiana radix before and after Wine-Processed by UHPLC-LTQ-Orbitrap MSn

Sun

et al. 2018

Molecules

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Gentiana radix is used in traditional Chinese medicine and has functions of clearing heat and drying dampness, as well as purging liver and gallbladder fire. A highly sensitive and effective strategy for rapid screening and identification of target constituents has been developed by using ultra high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap) in crude and wine-processed Gentiana radix. Based on the accurate mass measurement (<5 ppm), retention times, and MS fragmentation ions, 52 constituents were unambiguously or tentatively characterized from Gentiana radix, including 21 iridoids, 11 flavonoids, 19 xanthones, and a triterpenoid. This study demonstrated that the established method could be a rapid, effective analytical tool for screening and characterization of compounds in the complex systems of Gentiana radix. By comparing the structure and peak areas of chemical constituents in crude and wine-processed Gentiana radix, we found that some compounds in crude and wine-processed Gentiana radix were significantly different.

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“… 2017 , 2018 ; Wang et al. 2018 ; Liu et al. 2019 , 2020 ), and all 11 compounds were identified or deduced based on their precursor ions ([M-H] - ) and characteristic MS/MS fragmental data.…”

Section: Resultsmentioning

confidence: 99%

Ameliorative effects of the traditional Chinese medicine formula Qing-Mai-Yin on arteriosclerosis obliterans in a rabbit model

Zhang

Yuan

Song

et al. 2020

Pharmaceutical Biology

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Context Qing-Mai-Yin (QMY) is a clinically used herbal formula for treating arteriosclerosis obliterans (ASO). Objective To evaluate the chemical constituents and effects of QMY on ASO rabbit model. Materials and methods Forty-eight New Zealand rabbits were divided into six groups ( n = 8): normal (normal rabbits treated with 0.5% CMC-Na), vehicle (ASO rabbits treated with 0.5% CMC-Na), positive (simvastatin, 1.53 mg/kg), and QMY treatment (300, 600, and 1200 mg/kg). ASO rabbit model was prepared by high fatty feeding, roundly shortening artery, and bovine serum albumin immune injury. QMY (300, 600 and 1200 mg/kg) was orally administered for 8 weeks. The effects and possible mechanisms of QMY on ASO rabbits were evaluated by pathological examination, biochemical assays, and immunohistochemical assays. The compositions of QMY were analysed using HPLC-Q-TOF-MS/MS analysis. Results Compared to the vehicle rabbit, QMY treatment suppressed plaque formation and intima thickness in aorta, and decreased intima thickness, whereas increased lumen area of femoral artery. Additionally, QMY treatment decreased TC, TG and LDL, decreased CRP and ET, and increased NO and 6-K-PGF1α in serum. Furthermore, the potential mechanisms studied revealed that QMY treatment could suppress expression of TNF-α, IL-6, ICAM-1 and NF-κB in endothelial tissues, and increase IκB. In addition, HPLC analysis showed QMY had abundant anthraquinones, stilbenes, and flavonoids. Conclusion QMY has ameliorative effects on ASO rabbit, and the potential mechanisms are correlated to reducing inflammation and down-regulating NF-κB. Our study provides a scientific basis for the future application and investigation of QMY.

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Study on Chemical Constituents in Polygoni Cuspidati Folium and its Preparation by UPLC-ESI-Q-TOF-MS/MS

Cited by 18 publications

References 13 publications

UPLC–MS/MS method for the determination of the herb composition of Tangshen formula and the in vivo pharmacokinetics of its metabolites in rat plasma

UPLC–MS/MS method for the determination of the herb composition of Tangshen formula and the in vivo pharmacokinetics of its metabolites in rat plasma

Rapid Characterization and Identification of Chemical Constituents in Gentiana radix before and after Wine-Processed by UHPLC-LTQ-Orbitrap MSn

Ameliorative effects of the traditional Chinese medicine formula Qing-Mai-Yin on arteriosclerosis obliterans in a rabbit model

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