Study on Cell Adhesion Detection onto Biodegradable Electrospun PCL Scaffolds

Mijović, Budimir

doi:10.3993/jfbi03201202

Cited by 17 publications

(13 citation statements)

References 9 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Electrospun PU treated with NaOH was in that respect almost equal to amniotic membrane. This is different from our previous studies with fibroblast cell culture, where both electrospun PU and PCL treated with NaOH in regard to amniotic membrane, showed higher viability performance ( 41 ). Various concentrations and incubation time of NaOH used in different studies could influence the level of scaffold hydrophilicity obtained and could account for different types of response seen with various cell types.…”

Section: Discussioncontrasting

confidence: 99%

Synthetic vs natural scaffolds for human limbal stem cells

et al. 2015

View full text Add to dashboard Cite

AimTo investigate the impact of synthetic electrospun polyurethane (PU) and polycaprolactone (PCL) nanoscaffolds, before and after hydrolytic surface modification, on viability and differentiation of cultured human eye epithelial cells, in comparison with natural scaffolds: fibrin and human amniotic membrane.MethodsHuman placenta was taken at elective cesarean delivery. Fibrin scaffolds were prepared from commercial fibrin glue kits. Nanoscaffolds were fabricated by electrospinning. Limbal cells were isolated from surpluses of human cadaveric cornea and seeded on feeder 3T3 cells. The scaffolds used for viability testing and immunofluorescence analysis were amniotic membrane, fibrin, PU, and PCL nanoscaffolds, with or without prior NaOH treatment.ResultsScanning electron microscope photographs of all tested scaffolds showed good colony spreading of seeded limbal cells. There was a significant difference in viability performance between cells with highest viability cultured on tissue culture plastic and cells cultured on all other scaffolds. On the other hand, electrospun PU, PCL, and electrospun PCL treated with NaOH had more than 80% of limbal cells positive for stem cell marker p63 compared to only 27%of p63 positive cells on fibrin.ConclusionNatural scaffolds, fibrin and amniotic membrane, showed better cell viability than electrospun scaffolds. On the contrary, high percentages of p63 positive cells obtained on these scaffolds still makes them good candidates for efficient delivery systems for therapeutic purposes.

show abstract

Section: Discussioncontrasting

confidence: 99%

Synthetic vs natural scaffolds for human limbal stem cells

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Among diverse 3D structures, fibrous scaffolds are considered advantageous for the unique properties at micro and submicrometric scale (i.e., high surface area/volume ratio, reduced fiber diameter, high porosity and light weight) [1]. Their peculiar architecture is able to mimic extracellular matrix (ECM) properties influencing cell attachment and consequently being useful in functional systems as scaffolds for cell culture, tissue engineering and medical textiles [2].…”

Section: Introductionmentioning

confidence: 99%

“…However, being a semicrystalline hydrophobic polymer, PCL shows poor cell adhesion and stimulation of cell activities. This limitation can be overcome by providing hydrophilic groups to enhance both cell adhesive properties and creating an environment favorable for proliferation and cell interactions [2]. In this context, several strategies based on surface treatments or blending through the use of bioinspired materials have been exploring how to improve the hydrophilicity and so the biocompatibility of PCL electrospun fibers, without compromising material properties [7,8].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Polydopamine Coatings onto Poly–ε–Caprolactone Electrospun Fibers for the Fabrication of Bio-Electroconductive Interfaces

Zuppolini

Cruz‐Maya

Guarino

et al. 2020

JFB

View full text Add to dashboard Cite

In recent years, mussel adhesive proteins have attracted much attention because they can form strong adhesive interface interactions with various substrates in a wet environment. Inspired by their catechol- and amine-based molecular structure, polydopamine (PDA), a dopamine derived synthetic eumelanin polymer, was recognized as a suitable bio-interface coating. PDA was successfully used to improve adhesion due to the availability of copious functional groups for covalently immobilizing biomolecules and anchoring reactive species and ions. Recently, it has been demonstrated that PDA and its derivatives can be successfully used for the surface modification of implants interfaces to modulate in vitro cellular responses in order to enhance the in vivo functionality of biomedical implants (i.e., prosthesis). Herein, we propose the development of multifunctional scaffolds based on polyε–caprolactone (PCL) electrospun fibers coated with PDA via electro fluid dynamic methods, by optimizing polymerization/oxidation reactions capable of driving PDA self–assembly, and, ultimately, investigating the effects on cell response. Morphological analyses have confirmed the possibility to obtain different surface topographies as a function of the coating process while in vitro studies proved the ability of PDA coating to interact with cells no compromising in vitro viability. In perspective, in vitro conductive properties of fibers will be further investigated in order to validate their promising use as bioconductive interfaces for tissue engineering applications.

show abstract

“…13 It mimics the natural extracellular matrix in terms of the surface to volume ratio and high porosity and can be handled easily. 9,14 In another study, PCL biocompatibility and absence of immunological response after degradation has been reported. 15 PCL blending with gelatin (Gel) has been attractive to researchers as Gel is a natural component and might be suitable for improving PCL biological properties.…”

Section: Cell Culturementioning

confidence: 94%

Propagation of limbal stem cells on polycaprolactone and polycaprolactone/gelatin fibrous scaffolds and transplantation in animal model

et al. 2019

View full text Add to dashboard Cite

Introduction: This study was conducted to compare the effect of nanofibrous polycaprolactone (PCL) and PCL/gelatin (PCL/Gel) on limbal epithelial stem cell (LESC) and its efficiency for transplantation in animal model. Methods: PCL and PCL/Gel with a mass ratio of 70:30 and 50:50 was fabricated by electrospinning method. Human LESCs were cultured on PCL and PCL/Gel scaffolds and the effect of each scaffold on LESC proliferation, attachment and corneal epithelial regeneration in an animal model was evaluated, considering ease of use of scaffold and final transparency of the cornea. Results: Our data showed that PCL was more suitable than PCL/Gel for LESCs adherence, induction of epithelial morphology and proliferation. Histopathologic analysis of corneal sections from transplanted animals showed that epithelium was regenerated almost similar in PCL and PCL/Gel groups; however, vascularization and inflammation were significantly lower in the group receiving PCL. Conclusion: The represented data indicated the priority of PCL to PCL/Gel for the LESC attachment, proliferation and final outcome in an animal model of alkaline injury. This finding might be promising for cell therapy of corneal diseases.

show abstract

Study on Cell Adhesion Detection onto Biodegradable Electrospun PCL Scaffolds

Cited by 17 publications

References 9 publications

Synthetic vs natural scaffolds for human limbal stem cells

Synthetic vs natural scaffolds for human limbal stem cells

Optimization of Polydopamine Coatings onto Poly–ε–Caprolactone Electrospun Fibers for the Fabrication of Bio-Electroconductive Interfaces

Propagation of limbal stem cells on polycaprolactone and polycaprolactone/gelatin fibrous scaffolds and transplantation in animal model

Contact Info

Product

Resources

About