Propagation of limbal stem cells on polycaprolactone and polycaprolactone/gelatin fibrous scaffolds and transplantation in animal model

Sanie-Jahromi, Fatemeh; Eghtedari, Masoomeh; Mirzaei, Esmaeil; Jalalpour, Mohammad Hassan; Asvar, Zahra; Nejabat, Mahmood; Javidi-Azad, Fahimeh

doi:10.15171/bi.2020.06

Cited by 23 publications

(11 citation statements)

References 36 publications

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Section: Tensile Properties Of the Electrospun Scaffoldsmentioning

confidence: 92%

“…Another study, considered a combination of PCL with gelatin only, to be used for the propagation of limbal epithelial stem cells, in the repair of an animal model cornea. The mechanical performance of the scaffolds revealed 1.7 MPa of the pure PCL, while the blend PCL-gelatin scaffold showed an increase of the tensile strength of more than 2.5 MPa [50]. Figure 8 gives the calculated Young's moduli of the electrospun scaffolds at the strains of 5 and 10%, which were taken as the limit values of the elastic region for the PCL/25 wt % CF and all other scaffolds, respectively.…”

Section: Tensile Properties Of the Electrospun Scaffoldsmentioning

confidence: 99%

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Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Trcin

Zdraveva

Dolenec³

et al. 2020

Polymers

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Limbal Stem Cell Deficiency (LSCD) is a very serious and painful disease that often results in impaired vision. Cultivation of limbal stem cells for clinical application is usually performed on carriers such as amniotic membrane or surgical fibrin gel. Transplantation of these grafts is associated with the risk of local postoperative infection that can destroy the graft and devoid therapeutic benefit. For this reason, electrospun scaffolds are good alternatives, as proven to mimic the natural cells surroundings, while their fabrication technique is versatile with regard to polymer functionalization and scaffolds architecture. This study considers the development of poly(ε-caprolactone) (PCL) immune-compatible and biodegradable electrospun scaffolds, comprising cefuroxime (CF) or titanium dioxide (TiO2) active components, that provide both bactericidal activity against eye infections and support of limbal stem cells growth in vitro. The PCL/CF scaffolds were prepared by blend electrospinning, while functionalization with the TiO2 particles was performed by ultrasonic post-processing treatment. The fabricated scaffolds were evaluated in regard to their physical structure, wetting ability, static and dynamic mechanical behaviour, antimicrobial efficiency and drug release, through scanning electron microscopy, water contact angle measurement, tensile testing and dynamic mechanical analysis, antimicrobial tests and UV-Vis spectroscopy, respectively. Human limbal stem cells, isolated from surgical remains of human cadaveric cornea, were cultured on the PCL/CF and PCL/TiO2 scaffolds and further identified through immunocytochemistry in terms of cell type thus were stained against p63 marker for limbal stem cells, a nuclear transcription factor and cytokeratin 3 (CK3), a corneal epithelial differentiation marker. The electrospun PCL/CF and PCL/TiO2 successfully supported the adhesion, proliferation and differentiation of the cultivated limbal cells and provided the antimicrobial effect against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans.

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Section: Tensile Properties Of the Electrospun Scaffoldsmentioning

confidence: 92%

Section: Tensile Properties Of the Electrospun Scaffoldsmentioning

confidence: 99%

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Trcin

Zdraveva

Dolenec³

et al. 2020

Polymers

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“…Many studies have reported the printing of PCL scaffolds by electrospinning technology; however, owing to the poor control of the prepared bers, only few studies fabricated orthogonally aligned scaffolds that successfully resembled the corneal stroma. They simply used PCL scaffolds as a mechanical support for corneal substitutes without a particular structure to simulate the corneal stroma [39,47,48]. It is di cult to control the pattern of PCL bers precisely by electrospinning; more importantly, the electrospun bers have low transmittance because of their high density, which makes them unsuitable as corneal substitutes.…”

Section: Resultsmentioning

confidence: 99%

PCL scaffold combined with rat tail collagen type I to reduce keratocyte differentiation and prevent corneal stroma fibrosis after injury

Kong

Xie

et al. 2022

Experimental Eye Research

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The cornea is one of the major refractive eye components with signi cant functions, and its transparency is essential for clear vision. With regard to corneal injury, the corneal epithelium has a strong self-healing ability, while the corneal stroma is not capable of total self-repair. Therefore, preventing brosis and reducing keratocyte differentiation after injury have always been a challenge. The severe shortage of donor corneas for transplantation and transplant rejection prompted the development of corneal tissue engineering. In this study, we fabricated a poly(ε-caprolactone) (PCL) micro brous scaffold and infused the scaffold with rat tail collagen type I to obtain a 3D composite material. The PCL/collagen scaffold was designed to fabricate an optimal construct that simulates the stromal structure with properties that are most similar to the native cornea. The PCL scaffold has good mechanical properties, and infusion with rat tail collagen type I improved its biocompatibility. The results demonstrate that 3D composite material could reduce keratocyte differentiation, help achieve regular collagen distribution, and promote corneal repair.NanoSupplementarymaterialsandmethods.docx

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“…These composite systems have been shown to reduce neovascularization in inflammatory response. [49][50][51] Additional polymers used for cell delivery to the outermost layers of the cornea are further presented in Table 1.…”

Section: Electrospun Scaffoldsmentioning

confidence: 99%

Delivery of Cells to the Cornea Using Synthetic Biomaterials

Ross

Amaral

Taiyab

et al. 2022

Cornea

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The cornea is subject to a myriad of ocular conditions often attributed to cell loss or cell dysfunction. Owing to the superficial positioning of tissues composing the anterior segment of the eye, particularly the cornea, regenerative medicine in this region is aided by accessibility as compared with the invasive delivery methods required to reach deep ocular tissues. As such, cell therapies employing the use of carrier substrates have been widely explored. This review covers recent advances made in the delivery of stem cells, corneal epithelial cells, and corneal endothelial cells. Particular focus is placed on the most popular forms of synthetic scaffolds currently being examined: contact lenses, electrospun substrates, polymeric films, and hydrogels.

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Propagation of limbal stem cells on polycaprolactone and polycaprolactone/gelatin fibrous scaffolds and transplantation in animal model

Cited by 23 publications

References 36 publications

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

PCL scaffold combined with rat tail collagen type I to reduce keratocyte differentiation and prevent corneal stroma fibrosis after injury

Delivery of Cells to the Cornea Using Synthetic Biomaterials

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