Study on Biomarkers Related to the Treatment of Post-Stroke Depression and Alternative Medical Treatment Methods

Abstract

Purpose PSD is a syndrome that occurs after a stroke, which manifests as a series of depressive symptoms and corresponding physiological symptoms. Relevant studies have shown that the drug therapy is often accompanied by drug side effects and patient resistance. Acupuncture has attracted attention as a treatment method without adverse reactions of patients. The purpose of this study was to investigate the possible mechanism of action of acupuncture in PSD. Patients and Methods Download depression and stroke datasets from public databases. Bioinformatics methods were used to analyze the key gene targets related to stroke and depression. Functional enrichment analysis assesses important pathways. Further screen PSD-related biological pathways and genes. After the experimental model was established, the expression differences of key genes and related pathways were compared between the model group and the control group through acupuncture treatment and qPCR verification. Results Depression and stroke-related genes were obtained by bioinformatics methods, and then important biological processes and biological pathways related to depression and stroke were analyzed by GO and KEGG. And further screen out the disease targets closely related to PSD. In the follow-up animal experiments, we confirmed that acupuncture can intervene on these key pathways and targets, and then play a role in the targeted therapy of diseases. Conclusion The results of this study show that five genes (“NRBP1”, “SIRT1”, “BDNF”, “MAPK3”, “CREB1”.) and key biological pathways such as NFkB, PI3K/AKT activation, and MAPK are the keys to the occurrence and development of PSD biomarkers, which can also be therapeutically intervened by acupuncture.

Serum proteomic biomarker investigation of vascular depression using data-independent acquisition: a pilot study

“…Current research on protein markers of VaD is limited. The anti-aging gene Sirtuin 1 (SIRT1) was confirmed to be a depression and stroke-related gene ( Martins, 2016 ; Li et al, 2022 ). Sirtuin 1 is a critical nuclear deacetylase that participates in regulating the transcription of various transcription factors and cellular signal transduction proteins, involving inflammation, neurogenesis, glucose/cholesterol metabolism, and amyloidosis ( Lu et al, 2018 ; Man et al, 2019 ; Ministrini et al, 2021 ).…”

Luteolin and triptolide: Potential therapeutic compounds for post-stroke depression via protein STAT

“…Both bioinformatics and experimental studies have shown that activation of the PI3K/Akt signaling pathway tended to induce PSD [ 43 ]. Activation of the PI3K/Akt pathway could serve as a biomarker for PSD, which could protect nerves during cerebral ischemia/perfusion by inducing proliferation and differentiation of neural stem cells [ 40 , 44 ]. Various compounds, such as resveratrol, kaempferol (KPF), and NYT, have been shown to play a neuroprotective role by regulating the PI3K/Akt related pathways to reduce inflammatory factors and weaken microglial polarization [ 40 , 45 , 46 ].…”

Integrated genomic analysis of lung squamous cell carcinoma subtypes characterized by immunogenic cell death-relevant gene signature

“…Several R packages, including " org.Hs.Eg.Db," "ggplot2," and "clusterPro ler," were used to conduct Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment studies to look into the biological processes enriched by the acquired genes in critical modules 19 . The enriched GO or route keywords with notable changes were then scrutinized using a threshold p-value of 0.05.…”