Study on apoptosis effect of human breast cancer cell MCF-7 induced by lycorine hydrochloride via death receptor pathway

Yu-bin, JI; Yu, Miao; Zheng, Qi; Cui, Di; Xin, Guiliang; Wang, Bing; Jia, W.; Chang, Lin

doi:10.1016/j.jsps.2017.04.036

Cited by 36 publications

(24 citation statements)

References 11 publications

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“…Lycorine induces G1 phase cell cycle arrest in multiple myeloma and chronic myelocytic leukemia cell lines with downregulation of cyclin D and CDK4 [80,87,88]. Whereas, in ovarian cancer cell line HeyB1 and acute promyelocytic leukemia cell line HL-60 this compound induces cell cycle arrest at G2/M phase [65,85]. In response to growth factors, CDK4 and CDK6 combined with one of several D-type cyclins in early G1 phase.…”

Section: Cell Cycle Inhibitionmentioning

confidence: 99%

“…For the induction of extrinsic or death receptor pathway of apoptosis, lycorine was reported to activate death receptor ligands of the tumor necrosis factor (TNF) family TNF-α leading to caspase 8 activation [68,80,84]. It was also found that lycorine decreases the caspase-8 substrate Bid and significantly increases the expression level of truncated form of Bid protein (tBid) with a concomitant release of cytochrome C and activation of caspase-9 and caspase-3, indicating the Bid mediated crosstalk between mitochondria and death receptor apoptotic pathways upon lycorine treatment [84,85]. Lycorine also increases the portion of apoptotic cells in vivo xenografted tumor models.…”

Section: Apoptosis Inductionmentioning

confidence: 99%

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Lycorine: A prospective natural lead for anticancer drug discovery

Roy

Liang

Xiao

et al. 2018

Biomedicine & Pharmacotherapy

113

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Nature is the most abundant source for novel drug discovery. Lycorine is a natural alkaloid with immense therapeutic potential. Lycorine is active in a very low concentration and with high specificity against a number of cancers both in vivo and in vitro and against various drug-resistant cancer cells. This review summarized the therapeutic effect and the anticancer mechanisms of lycorine. At the same time, we have discussed the pharmacology and comparative structure-activity relationship for the anticancer activity of this compound. The researches outlined in this paper serve as a foundation to explain lycorine as an important lead compound for new generation anticancer drug design and provide the principle for the development of biological strategies to utilize lycorine in the treatment of cancers.

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Section: Cell Cycle Inhibitionmentioning

confidence: 99%

Section: Apoptosis Inductionmentioning

confidence: 99%

Lycorine: A prospective natural lead for anticancer drug discovery

Roy

Liang

Xiao

et al. 2018

Biomedicine & Pharmacotherapy

113

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“…The dichloromethane extracts, three alkaloids (lycorine, vittatine and montanine) exhibited potential antiproliferation activity in MCF7 breast cancer cells (Silva et al, 2008). Furthermore, it could induce the apoptosis of MCF7 (Ji et al, 2017).…”

Section: Discussionmentioning

confidence: 98%

Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling

Chen

2018

Bangladesh J Pharmacol

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Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling BJP IntroductionBreast cancer is the second leading cause of cancer death among women after lung cancer in the United States (DeSantis et al., 2014). In the treatment for breast cancer, some adverse effects could cause serious complications and affect the health and quality of life among survivors (Odle, 2014). Therefore, it is necessary to develop novel anti-cancer candidates for breast cancer.In the past several decades, natural compounds such as resveratrol (Sinha et al., 2016), epigallocatechin-3-gallate, oldenlandia diffusa and Ziziphus jujube have been demonstrated that possessed potential anti-breast cancer activity (Bonofiglio et al., 2016).Lycorine, known as a multifunctional compound, is extensively studied for its pharmacological activities, such as anti-tumor activity (Lamoral-Theys et al., 2010). Previous studies have shown that lycorine presented a potential anti-cancer activity in breast cancer via the inhibition of tumor growth and metastasis Ying et al., 2017). Src/FAK (focal adhesion kinase) -involved pathway and STAT3 (signal transducer and activator of transcription 3) signaling pathway were involved in growth, invasion and metastasis of breast cancer.However, Wnt/ -catenin signaling, a multifunctional pathway that has not been studied to be involved in proliferation, invasion and migration of breast cancer. This study is meant to confirm the inhibitory effects of lycorine on the proliferation, migration and invasion of MDA-MB-231 breast cancer cells and investigate the activation of Wnt/ -catenin signaling pathway involved. Materials and Methods Cell lines and reagentsHuman MDA-MB-231 breast cancer cells were obtained AbstractThis study was aimed to determine the effects of lycorine, a toxic crystalline alkaloid, on MDA-MB-231 breast cancer cells proliferation, migration and invasion, and to investigate the mechanism involved. The cells were cultured with different concentrations of lycorine in vitro. MTT assays were performed to determine the proliferation of cells. Transwell assays were performed to measure the migration and invasion of cells. The activation of Wnt/ -catenin signaling pathway and expression were assayed by Western blot. This study showed that proliferation, migration and invasion of MDA-MB-231 breast cancer cells could be inhibited by lycorine. Furthermore, we found that Wnt/ -catenin signaling was markedly blocked in MDA-MB-321 cells treated with lycorine. In conclusion, lycorine inhibits the proliferation, migration and invasion of MDA-MB-231 breast cancer cells that is associated with the suppression of Wnt/ -catenin signaling. Article Info

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“…28 Fas and FasL, which induced cell apoptosis, were activated in a report related to the effect of lycorine hydrochloride on MCF-7 cells. 29 In differentiated cells, DNMT1 activated p53 to regulate programmed cell death. 30 It has been confirmed that p53, as a dominant regulator of the apoptotic framework, can regulate extrinsic and intrinsic apoptosis pathway signaling cascades.…”

Section: Discussionmentioning

confidence: 99%

<p>Transcriptomics Analysis of the Tumor-Inhibitory Pathways of 6-Thioguanine in MCF-7 Cells via Silencing DNMT1 Activity</p>

Li¹,

An²,

Zhang³

et al. 2020

OTT

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Background: 6-thioguanine (6-TG), as a conventional "ancient" drug for the treatment of acute leukemia, has been proved to have extensive anti-tumor roles. This study was created to investigate the hidden function of 6-TG on the MCF-7 breast cancer cell line (ER+, PR+) and its mechanisms. Methods: MCF-7 cells were treated with 6-TG, and the IC50 value was measured by a cell counting kit-8 assay. Differentially expressed genes (DEGs) were confirmed by RNA-seq analysis. Apoptosis and cell cycle consequences were determined by flow cytometry and Western blot analyses. Results: The results showed that colony formation decreased markedly and the percentage of cell apoptosis increased after 6-TG treatment. DNMT1 mRNA and protein expression decreased, and FAS expression increased. Moreover, 6-TG also induced MCF-7 cells to undergo G2/M phase cell cycle arrest and upregulated CDKN1A (p21). Conclusion: Overall, our results suggest that 6-TG may induce FAS-mediated exogenous apoptosis and p21-dependent G2/M arrest by inhibiting the activity of DNMT1 in MCF-7 breast cancer cells.

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Study on apoptosis effect of human breast cancer cell MCF-7 induced by lycorine hydrochloride via death receptor pathway

Cited by 36 publications

References 11 publications

Lycorine: A prospective natural lead for anticancer drug discovery

Lycorine: A prospective natural lead for anticancer drug discovery

Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling

<p>Transcriptomics Analysis of the Tumor-Inhibitory Pathways of 6-Thioguanine in MCF-7 Cells via Silencing DNMT1 Activity</p>

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