Study on adhesion formation and the healing of colon anastomosis in rats with induced peritoneal sepsis

Silva, Silvana Marques e; Oliveira, Marcos Vinícius Melo de; Brandão, Alexandre Malta; Carneiro, Fabiana Pirani; Ferreira, Vânia Maria Moraes; Parra, Rogério Serafim; Féres, Omar; Sousa, João Batista de

doi:10.1590/s0102-86502011000800018

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…Both alcohol and sepsis are known to lead to weight loss after surgery, and their combination diminished the post-operative body mass in this study, and even at the 7 POD that weight wasn’t recovered [13,14]. Sepsis leads to a consumptive syndrome due to the inflammation and alcohol intake is responsible for malnutrition because of intestinal malabsorption and is also responsible for body fat reduction [13,14].…”

Section: Discussionmentioning

confidence: 86%

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Alcohol acute intoxication before sepsis impairs the wound healing of intestinal anastomosis: rat model of the abdominal trauma patient

et al. 2012

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IntroductionMost trauma patients are drunk at the time of injury. Up to 2% of traumatized patients develop sepsis, which considerably increases their mortality. Inadequate wound healing of the colonic repair can lead to postoperative complications such as leakage and sepsis.ObjectiveTo assess the effects of acute alcohol intoxication on colonic anastomosis wound healing in septic rats.MethodsThirty six Wistar rats were allocated into two groups: S (induction of sepsis) and AS (alcohol intake before sepsis induction). A colonic anastomosis was performed in all groups. After 1, 3 or 7 days the animals were killed. Weight variations, mortality rate, histopathology and tensile breaking strength of the colonic anastomosis were evaluated.ResultsThere was an overall mortality of 4 animals (11.1%), three in the group AS (16.6%) and one in the S group (5.5%). Weight loss occurred in all groups. The colon anastomosis of the AS group didn’t gain strength from the first to the seventh postoperative day. On the histopathological analysis there were no differences in the deposition of collagen or fibroblasts between the groups AS and S.ConclusionAlcohol intake increased the mortality rate three times in septic animals. Acute alcohol intoxication delays the acquisition of tensile strength of colonic anastomosis in septic rats. Therefore, acute alcohol intoxication before sepsis leads to worse prognosis in animal models of the abdominal trauma patients.

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Section: Discussionmentioning

confidence: 86%

“…Sepsis leads to a consumptive syndrome due to the inflammation and alcohol intake is responsible for malnutrition because of intestinal malabsorption and is also responsible for body fat reduction [13,14]. …”

Section: Discussionmentioning

confidence: 99%

Alcohol acute intoxication before sepsis impairs the wound healing of intestinal anastomosis: rat model of the abdominal trauma patient

et al. 2012

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“…Nevertheless, these patients frequently require surgical procedures, and the inadequate healing of their wounds can cause a number of complications, such as the dehiscence of sutures of the abdominal wall, which is an incident associated with high rates of morbidity and death. A decrease of tensile strength in intestinal anastomoses and sutures of the abdominal wall, has been shown in studies with rats with peritoneal sepsis [8][9][10] . Thus, the detection of factors relating to deficient healing in patients with sepsis will be able to improve therapeutic procedures 6 .…”

Section: Introductionmentioning

confidence: 88%

The effect of abdominal sepsis in the healing of skin on diabetic rats

Lima

Braz²,

Carvalho³

et al. 2015

J Surg Clin Res

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Background/purpose: There is an increasing discussion concerning the deleterious effect of hyperglycemia on the wound healing. Patients with sepsis are subjected not only to high risk of death, but are also vulnerable to developing problems related to deficient healing. The aim of this study was to examine the interference of abdominal sepsis and diabetes on the healing of skin in a rat experimental model. Methods: Wistar rats (Rattus norvegicus) weighing 282±34g randomly distributed into 4 groups of 6 animals each. Twelve animals were subjected to abdominal sepsis with cecal ligation and puncture (CLP) and 12 without sepsis. Six animals from each subgroup had induction of diabetes induced with streptozotocin 50 mg/kg, i.p. and six were non-diabetic. Six days after sepsis induction, a tensile strength test of skin scar from abdominal wall and histopatology of skin wound were carried out. Results: The tensile strength showed a significant difference between groups. The control group rats had a tensile strength of 219.0±12.4 gf/cm2, significantly higher than in the diabetes group (185.2±5,9gf/cm2) and in the sepsis group (107.0±9,8mg/cm2) respectively. The group of animals subjected to sepsis + diabetes had the lowest mean tensile strength (86.3±6,6gf/cm2), significantly lower than the results from other groups (p<0.0001). The isolated sepsis was more detrimental to wound healing than the isolated diabetes. The existence of the two comorbidities resulted in the lower tensile strength of the scar tissue. The control group rats exhibited an inflammatory reaction score (4.67±0.16) significantly lower (p=0.002) than the scores exhibited by the sepsis + diabetes group (5.98 ± 0.27), the sepsis group (5.04 ± 0.23) and the diabetes group (4.95 ± 0.32). Conclusion: Sepsis and diabetes, alone or in combination, negatively influenced the healing of skin wounds in rats. Therefore, the data indicates that sepsis and diabetes may operate as deleterious to the wound healing.

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