Study of visceral hypersensitivity in irritable bowel syndrome

Dong, Wen Zhu; Zou, Danping; Li, Zhao Shen; Zou, Xiao Ping; Zhu, Ai Yong; Xu, Guokai; Yin, Ning; Gong, Yan; Sun, Zhen; Man, Xiao Hua

doi:10.1111/j.1443-9573.2004.00168.x

Cited by 35 publications

(31 citation statements)

References 19 publications

(22 reference statements)

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“…Indeed, the cellularity of the lamina propria,2 34 35 the number of MCs in the colon2 8 9 or the terminal ileum6 7 36 and the numbers of IELs2 have been found to be significantly increased in IBS patients compared with healthy subjects. Interestingly, in several of these studies, these changes were more marked in the ileum or right colon, whereas the rectum often tended to be normal 6 9 34.…”

Section: Discussionmentioning

confidence: 99%

“…Among these, mast cells (MCs) are important candidates in IBS since they release several potent inflammatory and immune-modulating mediators that can alter nerve4 and muscle function 5. Quantitative assessments have identified infiltrates of MCs in the ileum6 7 and colon2 8 9 of IBS patients. It has also been shown that MC infiltration and release of mediators in the proximity of mucosal innervation may contribute to abdominal pain perception in IBS patients 8.…”

mentioning

confidence: 99%

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Mast cells and cellularity of the colonic mucosa correlated with fatigue and depression in irritable bowel syndrome

Piche

Saint–Paul

Dainese

et al. 2007

Gut

166

127

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Mast cells and cellularity of the colonic mucosa correlated with fatigue and depression in irritable bowel syndrome

Piche

Saint–Paul

Dainese

et al. 2007

Gut

166

127

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“…The manifestations of a decreased pain threshold and increased sensitivity of the rectum in IBS patients have been confirmed by large amounts of clinical and experimental data. However, the [18] . IBS patients show a significantly reduced tolerance of balloon dilatation in the colon and rectum, displaying increased pain intensity [19] .…”

Section: Discussionmentioning

confidence: 99%

Effects of electroacupuncture on corticotropin-releasing hormone in rats with chronic visceral hypersensitivity

Liu

Fang

et al. 2015

WJG

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Author contributions: Liu HR, Fang XY and Wu HG contributed equally to this work; Liu HR, Fang XY and Wu LY analyzed the data and wrote the paper; Liu HR, Li J, Weng ZJ and Guo XX established the animal experimental model and participated equally in animal treatment; Liu HR, Weng ZJ and Wu LY performed the molecular investigations; Li YG and Wu HG designed and coordinated the research.Supported by China Postdoctoral Science Foundation funded project, No.200801260, No.20080430117 Abstract AIM: To investigate the effect of electroacupuncture on corticotropin-releasing hormone (CRH) in the colon, spinal cord, and hypothalamus of rats with chronic visceral hypersensitivity. METHODS:A rat model of chronic visceral hypersensitivity was generated according to the internationally accepted method of colorectal balloon dilatation. In the 7 th week after the procedure, rats were randomly divided into a model group (MG), electroacupuncture group (EA), and sham electroacupuncture group (S-EA). After treatment, the abdominal withdrawal reflex (AWR) score was used to assess the behavioral response of visceral hyperalgesia. Immunohistochemistry (EnVision method), ELISA, and fluorescence quantitative PCR methods were applied to detect the expression of CRH protein and mRNA in the colon, spinal cord, and hypothalamus. RESULTS:The sensitivity of the rats to the colorectal distension stimulus applied at different strengths Basic StudyEffects of electroacupuncture on corticotropin-releasing hormone in rats with chronic visceral hypersensitivity (20-80 mmHg) increased with increasing stimulus strength, resulting in increasing AWR scores in each group. Compared with NG, the AWR score of MG was significantly increased (P < 0.01). After conducting EA, the AWR scores of the rats were decreased compared with MG rats. The relative expression of CRH mRNA in the colon, spinal cord, and hypothalamus of MG rats was significantly increased compared with NG rats (P < 0.01). CRH mRNA in the colon and spinal cord of EA and S-EA rats was decreased to varying degrees (P > 0.05) compared with normal rats (NG). However, the decrease in EA compared with MG rats was statistically significant (P < 0.01). The average optical density of CRH expression in the colon of the MG rats was significantly enhanced compared with NG (P < 0.05), while the average optical density of CRH expression in the EA and S-EA rats was significantly decreased compared with MG rats (P < 0.01, P < 0.05, respectively). Compared with MG rats, the CRH concentration in the spinal cord of EA rats was significantly reduced (P < 0.01), but there was no significant change in S-EA rats (P > 0.05). CONCLUSION:Electroacupuncture at the Shangjuxu acupoint was able to significantly reduce the visceral hypersensitivity in rats, and regulated the expression of CRH protein and mRNA in the colon, spinal cord and hypothalamus at different levels, playing a therapeutic role in this model of irritable bowel syndrome. Core tip: Visceral hypersensitivity is enhanced in irritable bowel syndrome (IBS) ra...

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“…Under electron microscope, there are many higher density granulars in the plasma. Active MMC is the key cell of gastrointestinal disfunction and abnormal sensitivity [12][13][14][15], producing large portions of chemical cytokine, participating in connections with nerve and immunosystems and in signal conduction within the brain-gut axis, and having an important role in intestinal hypersensitivity and dynamic disorder [16][17][18][19]. It could lead to functional abnormal movement-secretion in the intestine, and improve visceral hypersensitivity of pain.…”

Section: The Regulation Of Ea On Mast Cells In Ibs Model Ratsmentioning

confidence: 99%

Regulatory Mechanism of Electroacupuncture in Irritable Bowel Syndrome: Preventing MC Activation and Decreasing SP VIP Secretion

Jiang

Zhou

et al. 2007

Dig Dis Sci

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The efficacy of electroacupuncture (EA) for treating patients with diarrhea-predominant IBS has been confirmed in the authors' former research, but the regulatory mechanism of EA in IBS is still unknown. The aim of this study was to explore the relationship between the effect of EA on treating IBS rats and the activation and proliferation of mast cell (MC), the secretion of substance P(SP), and vasoactive intestinal polypeptide (VIP). The IBS rat model was set up with stress of binding limbs and colorectal distention. All rats were randomly assigned to four groups (Normal, Model, Tegaserod and EA). Hematoxylin and eosin staining has been used to observe the pathological change in the rats' colonic mucosa and an AWR scoring system has been applied to evaluate improvement of visceral hypersensitivity in various methods of the different groups. Toluidine blue improved method (TBI) and immunohistochemistry have also been involved in observations of mucous mast cells in the colon, change of c-fos positive cells, and secretion of SP, SPR, VIP, VIPR in the local colon. Firstly, the threshold of visceral sensitivity in the rats model with IBS was remarkably reduced (P < 0.01). The MC count in colonic mucosa and c-fos positive cells count increased significantly (P < 0.01) with positive correlation within each. Secondly, EA on ST-25 and Tegaserod pouring into the stomach can inhibit the proliferation and activation of MC in the colon and regulate secretion of SP, SPR, VIP, VIPR (P < 0.01, P < 0.05), while the effect of EA is obviously superior to Tegaserod. We concluded, firstly, that the abnormal proliferation and activation of mucous mast cells in the colon, and oversecretion of neuropeptides such as SP, VIP and their receptors could be one of key mechanisms of etiology of IBS. Secondly, the inhibition of activation and proliferation and the secretion of SP, VIP could be major effects of EA when treating rats with IBS.

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Study of visceral hypersensitivity in irritable bowel syndrome

Abstract: As altered rectal perception is present in almost all patients with IBS, it might be a reliable biological characteristic of the disease. Alterations in the MC and SP of the intestinal mucosa may be important factors in visceral hypersensitivity.

Cited by 35 publications

References 19 publications

Mast cells and cellularity of the colonic mucosa correlated with fatigue and depression in irritable bowel syndrome

Mast cells and cellularity of the colonic mucosa correlated with fatigue and depression in irritable bowel syndrome

Effects of electroacupuncture on corticotropin-releasing hormone in rats with chronic visceral hypersensitivity

Regulatory Mechanism of Electroacupuncture in Irritable Bowel Syndrome: Preventing MC Activation and Decreasing SP VIP Secretion

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