Study of uroproteins in myeloma patients by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)

Abstract

Samples of the urine of 10 myeloma patients with proteinuria were examined by SDS-PAGE. Light chain proteins of Bence Jones (B.J.) type were excreted by 7 patients as monomer (m.w. 20--26.5 x 10(3) Dalton, by 2 patients as a mixture of monomer and dimer and by one patient as dimer. By two-dimensional electrophoresis in SDS-PAG and subsequent electrophoresis in agarose containing kappa and lambda chain specific antibodies the immunological identity of monomeric and dimeric B.J. protein of one patient has been shown. The two-dimensional analysis has been proven a valuable procedure in cases with the excretion of complete monoclonal protein and B.J. protein at the same time.

Free Light-Chain Proteinuria and Normal Renal Histopathology and Function in 11 Dogs Exposed to Leishmania infantum, Ehrlichia canis, and Babesia canis

“…1,2 SDS-AGE and SDS-PAGE, electrophoretic techniques that separate proteins according to molecular mass, have demonstrated FLCs in the 20-26.5 kDa region. 3,4 The relationship between the identification of urinary FLCs and the development of renal disorders has been established in multiple myeloma, where renal amyloidosis (AL type), light-chain deposition disease, and myeloma cast nephropathy represent different renal manifestations of the same disease. 18 In dogs, FLCs have been observed in urine from 25 to 45% of patients with multiple myeloma or chronic lymphocytic leukemia.…”

“…2,3 Sodium dodecyl sulfate electrophoresis in humans identifies FLCs with a molecular mass of 20-26.5 kDa. 3,4 In dogs, identification of urine FLCs generally is carried out with the heat test, 5-7 but immunoprecipitation 7,8 and SDS-PAGE 9 also have been used.…”

Electrophoretic analysis of Bence Jones proteinuria

“…In two patients (both k), the proportion of LC D to LC M increased over a four-month period of medication (cyclophosphamide) possibly suggesting ªescapeº of the neoplastic clone from treatment [87]. SDS-PAGE (first dimension) in combination with agarose gel crossed immunoelectrophoresis (second dimension) was subsequently used to investigate BJP (n = 10) to identify the free LC and determine its molecular weight (M r 20 000±26 500) and size distribution (seven patients showed LC M only; 2, LC M + LC D; and 1, LC D only) [88]. In a methodological development, SDS-PAGE of BJP (n = 12) was demonstrated on horizontal ultrathin 4±25% polyacrylamide gradient gels using the Multiphor electrophoresis system (Amersham Pharmacia Biotech UK, Little Chalfont, Bucks, UK) ± the free LC and LC fragment (M r 15 000) were detected by silver staining following electrophoresis of unconcentrated urine and were disElectrophoresis 1999, 20, 1307±1324 Electrophoresis of BJP 1311 …”