Study of treatment with hyaluronic acid combined with platelet rich plasma in the in vivo regeneration of defects in articular discs of temporomandibular joint

Ankha, Milagros del Valle El Abras; Prado, Renata Falchete do; Lima, Vinícius de Almeida Barros; Carvalho, Yasmin Rodarte; Vasconcellos, Luana Marotta Reis de

doi:10.1016/j.ajoms.2020.03.009

Cited by 1 publication

(2 citation statements)

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“…Following the in-vitro experiments showing the therapeutic potential of the hPRP/HA in TMJ-OA, we evaluated the effects of the hPRP/HA in TMJ-OA animal model. A previous study also showed effect of hPRP and HA on the regeneration of defects in the articular disc of TMJ in a rabbit model [ 18 ]. However, in our study we induced an inflammatory condition by using a chemical mediator CFA to establish a TMJ-OA animal model.…”

Section: Discussionmentioning

confidence: 98%

“…Moreover, hPRP or HA or combined treatment exerted therapeutic efficacies against chronic pain reduction and cartilage and bone regeneration in different types of OA [ 16 , 17 ]. Recently, a study used the HA and PRP combination for the in-vivo regeneration of the articular disc in TMJ, but the researchers did not show anti-inflammatory or OA-like conditions and also not evaluate cartilage and bone regeneration [ 18 ]. Most studies have supported the clinical use of the PRP and HA combination for treating knee OA, and the results indicate that their combination provides better clinical improvement than PRP or HA monotherapy [ 17 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Platelet-derived biomaterial with hyaluronic acid alleviates temporal-mandibular joint osteoarthritis: clinical trial from dish to human

Peng,

Singh,

Tsai

et al. 2023

J Biomed Sci

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Background Bioactive materials have now raised considerable attention for the treatment of osteoarthritis (OA), such as knee OA, rheumatoid OA, and temporomandibular joint (TMJ) OA. TMJ-OA is a common disease associated with an imbalance of cartilage regeneration, tissue inflammation, and disability in mouth movement. Recently, biological materials or molecules have been developed for TMJ-OA therapy; however, ideal treatment is still lacking. In this study, we used the combination of a human platelet rich plasma with hyaluronic acid (hPRP/HA) for TMJ-OA therapy to perform a clinical trial in dish to humans. Method Herein, hPRP was prepared, and the hPRP/HA combined concentration was optimized by MTT assay. For the clinical trial in dish, pro-inflammatory-induced in-vitro and in-vivo mimic 3D TMJ-OA models were created, and proliferation, gene expression, alcian blue staining, and IHC were used to evaluate chondrocyte regeneration. For the animal studies, complete Freund’s adjuvant (CFA) was used to induce the TMJ-OA rat model, and condyle and disc regeneration were investigated through MRI. For the clinical trial in humans, 12 patients with TMJ-OA who had disc displacement and pain were enrolled. The disc displacement and pain at baseline and six months were measured by MRI, and clinical assessment, respectively. Results Combined hPRP/HA treatment ameliorated the proinflammatory-induced TMJ-OA model and promoted chondrocyte proliferation by activating SOX9, collagen type I/II, and aggrecan. TMJ-OA pathology–related inflammatory factors were efficiently downregulated with hPRP/HA treatment. Moreover, condylar cartilage was regenerated by hPRP/HA treatment in a proinflammatory-induced 3D neocartilage TMJ-OA-like model. During the animal studies, hPRP/HA treatment strongly repaired the condyle and disc in a CFA-induced TMJ-OA rat model. Furthermore, we performed a clinical trial in humans, and the MRI data demonstrated that after 6 months of treatment, hPRP/HA regenerated the condylar cartilage, reduced disc displacement, alleviated pain, and increased the maximum mouth opening (MMO). Overall, clinical trials in dish to human results revealed that hPRP/HA promoted cartilage regeneration, inhibited inflammation, reduced pain, and increased joint function in TMJ-OA. Conclusion Conclusively, this study highlighted the therapeutic potential of the hPRP and HA combination for TMJ-OA therapy, with detailed evidence from bench to bedside. Trial registration Taipei Medical University Hospital (TMU-JIRB No. N201711041). Registered 24 November 2017. https://tmujcrc.tmu.edu.tw/inquiry_general.php.

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Section: Discussionmentioning

confidence: 98%