Study of total phenol, flavonoid contents and phytochemical screening of methanolic crude extracts of two weed plants

Shreshtha, Shah; Jain, Anushi; Joshi, Ambika Nitesh; Joshi, Nitesh; Anupma, Harshal

doi:10.21746/1651-1654

Cited by 11 publications

(8 citation statements)

References 1 publication

(1 reference statement)

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“…Phenolic and flavonoid compounds present in plants are associated with medicinal properties. Higher concentration of both phenolics and flavonoids triggers the pharmaceutical and biological attributes of a particular plant (Baba & Malik, 2015; Shreshtha et al, 2017). The discrimination in TPC and TFC was probably due to solvent polarity interaction with heterogeneous structural features of phytochemicals.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts

William

Park

Mumtaz

et al. 2019

PeerJ

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Background Diabetes mellitus type II (DMT-2) is a widely spread metabolic disorder both in developed and developing countries. The role of oxidative stress is well established in DMT-2 pathogenesis. The synthetic drugs for DMT-2 are associated with serious side complications. Antioxidant and α-glucosidase inhibitory actions of phytochemicals from various plant species are considered as an alternative to synthetic drugs for DMT-2 management. The present study aimed to evaluate the antioxidant activity, α-glucosidase inhibitory potential and phytochemical profiling of Hyophorbe lagenicaulis. Methods The total phenolic and flavonoid contents, in vitro antioxidant activity (α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging and phosphomolybdenum method) and α-glucosidase inhibition of ultrasonicated hydroethanolic H. lagenicaulis leaf extracts were determined spectrophotometrically. The results of DPPH assay and α-glucosidase inhibition were reported in terms of IC50 value. The phytochemical profiling was accomplished by UHPLC-Q-TOF/MS/MS technique. Results and Discussion Findings leaped 60% ethanolic extract as rich fraction regarding total phenolic and flavonoid contents. The 60% ethanolic fraction was a promising source of natural antioxidants and α-glucosidase inhibitory agents as indicated by anti-radical and enzyme inibitory activities. Kaempferol, rutin, hesperetin 5-O-glucoside, kaempferol-coumaroyl-glucoside, luteolin 3-glucoside, Isorhamnetin-3-O-rutinoside, trimethoxyflavone derivatives and citric acid were identified by UHPLC-Q-TOF-MS/MS. These compounds were believed to be responsible for the strong antioxidant and enzyme inhibitory activity of plant extracts. The extensive metabolite profiling of H. lagenicaulis was carried out the first time as never reported previously. The H. lagenicaulis might be an appropriate choice to manage diabetes mellitus in an alternate way. The findings may be further exploited extensively for toxicity evaluation to proceed with functional food development having antidiabetic attributes.

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Section: Discussionmentioning

confidence: 99%

Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts

William

Park

Mumtaz

et al. 2019

PeerJ

View full text Add to dashboard Cite

show abstract

“…Phenolic and flavonoid compounds present in plants are associated with medicinal properties. Higher concentration of both phenolics and flavonoids triggers the pharmaceutical and biological attributes of a particular plant (Baba and Malik, 2015;Shreshtha et al, 2017). The discrimination in TPC and TFC was probably due to solvent polarity interaction with heterogeneous structural features of phytochemicals.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts (v0.1)"

2019

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Background Diabetes mellitus type II (DMT-2) is widely spread metabolic disorder both in developed and developing countries. The role of oxidative stress is well established in DMT-2 pathogenesis. The synthetic drugs for DMT-2 are associated with serious side complications. Antioxidant and α-glucosidase inhibitory actions of phytochemicals from various plant species are considered as an alternative to synthetic drugs for DMT-2 management. The present study aimed to evaluate the antioxidant activity, α-glucosidase inhibitory potential and phytochemical profiling of Hyophorbe lagenicaulis. Methods The total phenolic and flavonoid contents, in vitro antioxidant activity (DPPH assay and phosphomolybedenum method) and α-glucosidase inhibition of ultrasonicated hydroethanolic Hyophorbe lagenicaulis leaf extracts were determined spectrophotometrically. The results of DPPH assay and αglucosidase inhibition were reported in terms of IC 50 value. The phytochemical profiling was accomplished by UHPLC-Q-TOF/MS/MS technique. Results and Discussion Findings leaped 60% ethanolic extract as rich fraction regarding total phenolic and flavonoid contents. The 60% ethanolic fraction was promising source of natural antioxidants and αglucosidase inhibitory agents as indicated by anti-radical and enzyme inibitory activities. Kaempferol, rutin, hesperetin 5-O-glucoside, kaempferol-coumaroyl-glucoside, luteolin 3-glucoside, Isorhamnetin-3-Orutinoside, trimethoxyflavone derivatives and citric acid were identified by UHPLC-Q-TOF-MS/MS. These compounds were believed to be responsible for the strong antioxidant and anti-enzymatic activity of plant extracts. The extensive metabolite profiling of H. lagenicaulis was carried out first time as never reported previously. The H. lagenicaulis might be an appropriate choice to manage diabetes mellitus in alternate way. The findings may be further exploited extensively for toxicity evaluation to proceed with functional food development having antidiabetic attributes.

show abstract

“…246 The following references reported chemical compositions (some are very detailed) of known natural products. 233,234,[247][248][249][250] Special method for detection and quantification of gallic acid in the plant. 251 Very extensive work was invested in this research, especially in spectroscopic characterization of the reported compound.…”

Section: Chemical Compositionmentioning

confidence: 99%

Amaranthaceae plants of Israel and Palestine: Medicinal activities and unique compounds

Azab¹

2020

ECB

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Study of total phenol, flavonoid contents and phytochemical screening of methanolic crude extracts of two weed plants

Cited by 11 publications

References 1 publication

Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts

Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts

Peer Review #1 of "Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts (v0.1)"

Amaranthaceae plants of Israel and Palestine: Medicinal activities and unique compounds

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