Peer Review #1 of "Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts (v0.1)"

Abstract

Background Diabetes mellitus type II (DMT-2) is widely spread metabolic disorder both in developed and developing countries. The role of oxidative stress is well established in DMT-2 pathogenesis. The synthetic drugs for DMT-2 are associated with serious side complications. Antioxidant and α-glucosidase inhibitory actions of phytochemicals from various plant species are considered as an alternative to synthetic drugs for DMT-2 management. The present study aimed to evaluate the antioxidant activity, α-glucosidase inhibitory potential and phytochemical profiling of Hyophorbe lagenicaulis. Methods The total phenolic and flavonoid contents, in vitro antioxidant activity (DPPH assay and phosphomolybedenum method) and α-glucosidase inhibition of ultrasonicated hydroethanolic Hyophorbe lagenicaulis leaf extracts were determined spectrophotometrically. The results of DPPH assay and αglucosidase inhibition were reported in terms of IC 50 value. The phytochemical profiling was accomplished by UHPLC-Q-TOF/MS/MS technique. Results and Discussion Findings leaped 60% ethanolic extract as rich fraction regarding total phenolic and flavonoid contents. The 60% ethanolic fraction was promising source of natural antioxidants and αglucosidase inhibitory agents as indicated by anti-radical and enzyme inibitory activities. Kaempferol, rutin, hesperetin 5-O-glucoside, kaempferol-coumaroyl-glucoside, luteolin 3-glucoside, Isorhamnetin-3-Orutinoside, trimethoxyflavone derivatives and citric acid were identified by UHPLC-Q-TOF-MS/MS. These compounds were believed to be responsible for the strong antioxidant and anti-enzymatic activity of plant extracts. The extensive metabolite profiling of H. lagenicaulis was carried out first time as never reported previously. The H. lagenicaulis might be an appropriate choice to manage diabetes mellitus in alternate way. The findings may be further exploited extensively for toxicity evaluation to proceed with functional food development having antidiabetic attributes.