Study of the Structure, Oxygen-Transporting Functions, and Ionic Composition of Erythrocytes at Vascular Diseases

Grunyushkin

Biotechnology & Biotechnological Equipment

et al. 2016

The aim of this study was to investigate the relationship between the composition and state of erythrocytic lipids and oxygen-binding properties of erythrocyte haemoglobin-derived haematoporphyrin under hypoxia. The results from this study showed that hypoxia causes a profound change in the composition of the lipids that form the bilayer of erythrocyte membranes, as well as in the functional characteristics of erythrocytes. The detected changes correlate with morphological reconstruction of erythrocytes and, consequently, affect the oxygen-transport properties of erythrocytes. The obtained results showed that the lipid phase plays an important role in the functioning of erythrocytes in health as well as in event of development of ischemic heart disease and other pathological processes that accompany hypoxia.

Section: Effect Of Hypoxia On the Fatty-acid Composition Of Erythrocymentioning

confidence: 99%

“…Different pathologies cause changes in the amount, distribution and conformation of Hb [4][5][6][7]. Many pathological states are accompanied by development of hypoxia.…”

Section: Introductionmentioning

confidence: 99%

Effect of hypoxia on the composition and state of lipids and oxygen-transport properties of erythrocyte haemoglobin

Grunyushkin

Biotechnology & Biotechnological Equipment

et al. 2016

“…Changes in the phospholipid and FA composition of the erythrocyte lipids point to structural rearrangements in the membrane of erythrocytes and thus to the functional changes on the part of the erythrocyte as a whole, as well as on the part of the primary oxygen-transport protein of RBCs -haemoglobin (membrane-bound and cytoplasmic form) [4,5]. Our data obtained by RS suggested that these changes are linked to disruption of the conformation of haemoglobin and primarily its heme structures.…”

Section: Changes In the Oxygen-binding Capacity Of Erythrocyte Haemogmentioning

confidence: 65%

“…Many studies [1][2][3], including ours [4][5][6], have provided evidence that the lipid phase plays an active role in the regulation of the oxygen-transport function of haemoglobin during hypoxia, which is the cause and consequence of many cardiovascular diseases [7]. However, the human body often faces a situation when in some tissues and, in particular, in the blood, there may be an excessive amount of oxygen.…”

Section: Introductionmentioning

confidence: 99%

The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function

Biotechnology & Biotechnological Equipment

Revina

et al. 2018

The aim of this study was to investigate the effect of hyperoxia, calcium ions and pH value on the composition of major phospholipids in human erythrocyte membranes and erythrocytes' oxygentransport function. To create a model of hyperoxia, we saturated the incubated mixture with oxygen by constant passing of oxygen-air mixture through the incubation medium. To assess the effect of elevated calcium ion concentrations, CaCl 2 was added to the incubation medium. An incubation medium with different pH was used to study the effect of various pH values. Lipids were extracted from erythrocytes and chromatographic separation was carried out in a thin layer of silica gel deposited on a glass plate. The thiobarbituric acid (TBA)-active products and the content of diene conjugates (DC) in erythrocytes were determined. The oxygen-binding capacity of haemoglobin was evaluated using Raman spectroscopy. The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin. It should be noted that a combination of Ca 2+ ions and change in the pH value intensify the processes associated with disruption of phospholipids' composition. The findings indicate that the lipid phase is one of the key elements in the functioning of erythrocytes in norm as well as during development of various pathological processes.

“…The use of Raman spectroscopy (RS) made it possible to identify changes in the conformation of hemoporphyrin in patients with severe arterial hypertension and under the therapeutic interval hypoxia in patients with stable effort [4] and in patients with circulatory failure [5–7]. …”

Section: Introductionmentioning

confidence: 99%

Role of Membrane Lipids in the Regulation of Erythrocytic Oxygen-Transport Function in Cardiovascular Diseases

BioMed Research International

Revina

et al. 2016

Self Cite

The composition and condition of membrane lipids, the morphology of erythrocytes, and hemoglobin distribution were explored with the help of laser interference microscopy (LIM) and Raman spectroscopy. It is shown that patients with cardiovascular diseases (CVD) have significant changes in the composition of their phospholipids and the fatty acids of membrane lipids. Furthermore, the microviscosity of the membranes and morphology of the erythrocytes are altered causing disordered oxygen transport by hemoglobin. Basic therapy carried out with the use of antiaggregants, statins, antianginals, beta-blockers, and calcium antagonists does not help to recover the morphofunctional properties of erythrocytes. Based on the results the authors assume that, for the relief of the ischemic crisis and further therapeutic treatment, it is necessary to include, in addition to cardiovascular disease medicines, medication that increases the ability of erythrocytes' hemoglobin to transport oxygen to the tissues. We assume that the use of LIM and Raman spectroscopy is advisable for early diagnosis of changes in the structure and functional state of erythrocytes when cardiovascular diseases develop.