Study of the protein distribution in the pig lens cross section by Raman spectroscopy

Medina-Gutiérrez, C.; Frausto-Reyes, Claudio; Quintanar-Stephano, J.L; Sato-Berrú, R. Y.; Barbosa-Garcı́a, Oracio

doi:10.1016/j.saa.2003.07.010

Cited by 2 publications

(3 citation statements)

References 7 publications

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“…Due to the complexity of this tissue, we used previous spectroscopic investigations to confirm the assignments obtained [2,3,10,12]. Raman spectra were collected in the range between 1800 and 800 cm −1 that is a characteristic of crystalline lens and functional groups vibrations.…”

Section: Resultsmentioning

confidence: 99%

“…For analyzing biological tissues, Raman spectroscopy offers a significant advantage over other techniques since water has a very weak Raman signal. For this reason, besides the fact that it is a non-invasive technique, it has been applied for diagnostic and characterization of distinct biological process such as blood, serum, breast cancer, human astherosclerosis, as well as eye [9,10] among others. This fact allows a characteristic Raman spectrum to each molecule, and consequently, each biological tissue can present specific Raman spectral signatures [3].…”

Section: Introductionmentioning

confidence: 99%

“…The Raman spectrum from a canine cataract lens is also shown and the main differences observed are discussed. The comprehension of the structure of the lens proteins, its arrangement, and interactions within the lens are fundamental to investigate the mechanisms of cataract formation and consequently aspects relative to the preservation inhibiting the disease process [10,11]. Many studies have shown that the disease may depends on many factors such as changes in the nature of the proteins lens, compactness of the fibers, and others physicochemical variations [1,2].…”

Section: Introductionmentioning

confidence: 99%

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In vitro Raman spectroscopy of healthy mammals crystalline lenses

et al. 2005

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Abstract:The crystalline lens is a transparent biological material, and a complex inhomogeneous optical element in the vision system of mammals. It is a fiber cell structures [1]. Approximately 90% of the fiber cells composition are proteins responsible for the high refractive index of the lenses. This paper presents Raman spectroscopy as a tool for identification of the proteins and further diagnostic of disease in the lenses. Would all mammal lenses be made of the same proteins? This is the question motivating this work. Raman spectroscopy is a rapid and nondestructive optical method for providing chemical information on molecular structures [2], and has been successfully applied to a variety of biological systems [2,3]. Our aim here is to investigate the protein average distribution in the lenses of distinct mammals by following main differences in the Raman spectral signature. The Raman signatures of healthy crystalline lenses from canine, mouse, pig, rabbit and horse were characterized in vitro. The results indicate that there are no substantial differences at all animals studied.

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Section: Resultsmentioning

confidence: 99%